Genome-wide analysis of MATE transporters and response to metal stress in <i>Cajanus cajan</i>

Dong, Biying; Niu, Lili; Meng, Dong; Song, Zhihua; Wang, Litao; Jian, Yue; Fan, Xiao-Hong; Dong, Mingzhu; Yang, Qing; Fu, Yujie

doi:10.1080/17429145.2019.1620884

Cited by 28 publications

(17 citation statements)

References 57 publications

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“…We discovered 20 MATE-encoding genes from the wheat genome in this study, which can be classi ed into three main subfamilies, which is consistent with previous classi cation of MATE genes in [70] (Fig. 3).…”

Section: Discussionsupporting

confidence: 90%

“…It has been reported that MATE proteins play an important role in the e ux of plant secondary metabolites, acting as gatekeepers for cells by regulating the in ow of useful substances and the exudation of harmful ones [16]. MATE family genes have been studied in some plant species [33,36,[68][69][70], but not in wheat. Wheat is a protein-rich grain crop.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of MATE Gene Family And Its Role In Drought, Heat, And Salt Tolerance In Wheat (Triticum Aestivum L.)

Amoah

Seo

2021

Preprint

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To explore the response of multidrug and toxic compound extrusion (MATE) proteins to drought, heat, and salt stress in wheat, a genome-wide identification and expression study was performed. 20 MATE genes located on 4 of the 12 chromosomes were identified and categorized into four (I-1V) subfamilies, based on phylogenetic analysis. Wheat MATE family expansion was primarily driven by whole-genome duplication (WGD) and tandem events. In the same subfamily, gene exon-intron structures and motif composition are more similar. TaMATE genes had cis-acting elements that were implicated in stress and defense response. Tae-miR5175e was identified as the highly expressed miRNA that targets TaMATEs by miRNA prediction. When compared to controls, the relative expression patterns of seven TaMATE genes were substantially elevated during drought stress. TaMATE2, 10, 13, and 14 expression levels considerably elevated after 15 days (d) of heat stress, whereas TaMATE2, 14, 18, and 20 expression levels were highly upregulated following 15 d of salt stress treatment, indicating the crucial role of TaMATEs under these abiotic stress conditions. Furthermore, drought, heat, and salt stress decreased wheat water content, but increased malondialdehyde (MDA), electrolyte leakage (EL), and proline content, whereas the expression of the 7 putative MATE genes was correlated with physio-biochemical indicators of these stress conditions. The findings contribute to a better understanding of the complexities of MATEs and present a theoretical base for future MATE gene discovery and application in wheat and other crop species.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Characterization of MATE Gene Family And Its Role In Drought, Heat, And Salt Tolerance In Wheat (Triticum Aestivum L.)

Amoah

Seo

2021

Preprint

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“…The MATE transporters are widely conserved in eukaryotic and prokaryotic organisms (Chen et al 2015). Recently, the functions of many MATE transporters have been illustrated in plants (Takanashi et al 2014), including transport of secondary metabolites such as alkaloids (Shoji et al 2009), xenobiotic extrusion (Miyauchi et al 2017), flavonoids (Debeaujon et al 2001), anthocyanin (Pérez‐Díaz et al 2014), detoxification of heavy metals (Li et al 2002, Dong et al 2019), regulation of disease resistance (Sun et al 2011), efflux of plant hormones such as abscisic acid (ABA) (Zhang et al 2014), salicylic acid (SA) biosynthesis (Yamasaki et al 2013), iron translocation (Yokosho et al 2009), aluminum (Al) detoxification (Fujii et al 2012), turgor regulation (Zhang et al 2017), hypocotyl cell elongation (Wang et al 2015), enhanced organ initiation (Burko et al 2011), which indicates the importance of MATE transporters in plants. Owing to these properties, several studies have been conducted to characterize MATE proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike mammalian genomes that carry only a few MATE genes (e.g. 5 in mouse, 11 in human), plant genomes encode a large number of MATE genes, for instance, 56 in Arabidopsis thaliana (Li et al 2002), 53 in Oryza sativa (Tiwari et al 2014), 70 in Medicago truncatula (Zhao and Dixon 2009), 128 in Gossypium hirsutum (Lu et al 2019), 49 in Zea mays (Zhu et al 2016), 48 in potato (Li et al 2019), 67 in tomato (dos Santos et al 2017), 67 in Pigeon pea (Dong et al 2019), 71 in Populus trichocarpa (Li et al 2017) and 117 in soybean (Liu et al 2016). Besides, MATE gene family has also been identified in other plant species such as Sorghum bicolor (Sivaguru et al 2013), wheat (Garcia‐Oliveira et al 2018), Setaria viridis (Ribeiro et al 2017), persimmon (Yang et al 2016), cabbage (Wu et al 2014), Nicotiana tabacum (Morita et al 2009) and Lotus japonicus (Takanashi et al 2013), but no work has been reported in flax ( Linum usitatissimum ) so far.…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide identification and expression analysis of detoxification efflux carriers (DTX) genes family under abiotic stresses in flax

Ali

Saand

Khan

et al. 2020

Physiologia Plantarum

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The detoxification efflux carriers (DTX)/multidrug and toxic compound extrusion (MATE) transporters encompass an ancient gene family of secondary transporters involved in the process of plant detoxification. A genome‐wide analysis of these transporters was carried out in order to better understand the transport of secondary metabolites in flaxseed genome (Linum usitassimum). A total of 73 genes coding for DTX/MATE transporters were identified. Gene structure, protein domain and motif organization were found to be notably conserved over the distinct phylogenetic groups, showing the evolutionary significant role of each class. Gene ontology (GO) annotation revealed a link to transporter activities, response to stimulus and localizations. The presence of various hormone and stress‐responsive cis‐regulatory elements in promoter regions could be directly correlated with the alteration of their transcripts. Tertiary structure showed conservation for pore size and constrains in the pore, which indicate their involvement in the exclusion of toxic substances from the cell. MicroRNA target analysis revealed that LuDTXs genes were targeted by different classes of miRNA families. Twelve LuDTX genes were chosen for further quantitative real‐time polymerase chain reaction analysis in response to cold, salinity and cadmium stress at 0, 6, 12 and 24 hours after treatment. Altogether, the identified members of the DTX gene family, their expression profile, phylogenetic and miRNAs analysis might provide opportunities for future functional validation of this important gene family in flax.

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“…When the pigeonpea seedlings were 3 weeks old, we selected pigeonpea seedlings with consistent growth status for Al treatment. According to our previous research, we performed Al treatment at a concentration of 50 µM [26]. Samples of roots were collected at 0, 3, 6, and 12 h after Al treatment, frozen immediately in liquid nitrogen, and then stored at −80 • C for RNA isolation.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Time Series RNA-seq in Pigeonpea Revealed the Core Genes in Metabolic Pathways under Aluminum Stress

Gao

Dong

Cao

et al. 2020

Genes

Self Cite

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Pigeonpea is an important economic crop in the world and is mainly distributed in tropical and subtropical regions. In order to further expand the scope of planting, one of the problems that must be solved is the impact of soil acidity on plants in these areas. Based on our previous work, we constructed a time series RNA sequencing (RNA-seq) analysis under aluminum (Al) stress in pigeonpea. Through a comparison analysis, 11,425 genes were found to be differentially expressed among all the time points. After clustering these genes by their expression patterns, 12 clusters were generated. Many important functional pathways were identified by gene ontology (GO) analysis, such as biological regulation, localization, response to stimulus, metabolic process, detoxification, and so on. Further analysis showed that metabolic pathways played an important role in the response of Al stress. Thirteen out of the 23 selected genes related to flavonoids and phenols were downregulated in response to Al stress. In addition, we verified these key genes of flavonoid- and phenol-related metabolism pathways by qRT-PCR. Collectively, our findings not only revealed the regulation mechanism of pigeonpea under Al stress but also provided methodological support for further exploration of plant stress regulation mechanisms.

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Genome-wide analysis of MATE transporters and response to metal stress in Cajanus cajan

Cited by 28 publications

References 57 publications

Characterization of MATE Gene Family And Its Role In Drought, Heat, And Salt Tolerance In Wheat (Triticum Aestivum L.)

Characterization of MATE Gene Family And Its Role In Drought, Heat, And Salt Tolerance In Wheat (Triticum Aestivum L.)

Genome‐wide identification and expression analysis of detoxification efflux carriers (DTX) genes family under abiotic stresses in flax

Time Series RNA-seq in Pigeonpea Revealed the Core Genes in Metabolic Pathways under Aluminum Stress

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