Genome-wide analysis of microRNA and mRNA expression signatures in cancer

Li, Minghui; Fu, Shengbo; Xiao, Huasheng

doi:10.1038/aps.2015.67

Cited by 65 publications

(47 citation statements)

References 194 publications

(177 reference statements)

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“…MicroRNAs demonstrate exceptional stability even in difficult biological samples such as formalin-fixed paraffin-embedded tissue sections, blood, and urine [30, 31] and have been used as prognostic biomarkers in a number of cancers [32]. Moreover, several recent reports suggest that DCLK1 regulates EMT through a miRNA-dependent mechanism, which has also been confirmed in pancreatic and colon cancer using tumor xenograft models [25, 33].…”

Section: Discussionmentioning

confidence: 99%

Survival of Patients with Gastrointestinal Cancers Can Be Predicted by a Surrogate microRNA Signature for Cancer Stem–like Cells Marked by DCLK1 Kinase

Weygant

et al. 2016

Cancer Research

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DCLK1 is a gastrointestinal (GI) tuft cell kinase that has been investigated as a biomarker of cancer stem-like cells in colon and pancreatic cancers. However, its utility as a biomarker may be limited in principle by signal instability and dilution in heterogeneous tumors, where the proliferation of diverse tumor cell lineages obscures the direct measurement of DCLK1 activity. To address this issue, we explored the definition of a microRNA signature as a surrogate biomarker for DCLK1 in cancer stem-like cells. Utilizing RNA/miRNA sequencing datasets from the Cancer Genome Atlas, we identified a surrogate 15-miRNA expression signature for DCLK1 activity across several GI cancers, including colon, pancreatic and stomach cancers. Notably, Cox regression and Kaplan-Meier analysis demonstrated that this signature could predict the survival of patients with these cancers. Moreover, we identified patient subgroups that predicted the clinical utility of this DCLK1 surrogate biomarker. Our findings greatly strengthen the clinical significance for DCLK1 expression across GI cancers. Further, they provide an initial guidepost toward the development of improved prognostic biomarkers or companion biomarkers for DCLK1-targeted therapies to eradicate cancer stem-like cells in these malignancies.

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Section: Discussionmentioning

confidence: 99%

Survival of Patients with Gastrointestinal Cancers Can Be Predicted by a Surrogate microRNA Signature for Cancer Stem–like Cells Marked by DCLK1 Kinase

Weygant

et al. 2016

Cancer Research

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“…Ribonucleic acid (RNA) biomarkers comprising different coding and noncoding transcriptome such as messenger RNA (mRNA), microRNA (miRNA), and long noncoding RNA (lncRNA) are becoming progressively crucial in understanding disease diagnosis and prognosis due to their recognized physiological role in gene expression and regulation . Dysregulation of these RNA biomarkers is involved in the initiation and progression of several diseases including cancers .…”

Section: Introductionmentioning

confidence: 99%

RNA Biomarkers: Diagnostic and Prognostic Potentials and Recent Developments of Electrochemical Biosensors

et al. 2017

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Ribonucleic acids (RNAs) are considered as effective and minimally invasive biomarkers for disease diagnosis and prognosis due to their critical role in the regulation of different cellular processes. Over the past several years, the rapid progress in RNA biomarker research has resulted in the development of a large number of high‐performance RNA‐detection methods. Most of these methods are based on molecular‐biology techniques such as quantitative reverse transcription polymerase chain reaction (RT‐qPCR), microarrays, and RNA sequencing. In recent years, considerable attention has also been dedicated to developing RNA biosensors, exploiting micro‐ and nanofabrication technologies, and various readout strategies, including electrochemical and optical transducers. Here, the recent developments of RNA biosensors are concisely reviewed with a special emphasis on electrochemical‐detection approaches. The following points are also highlighted: i) all the types of clinically relevant RNAs (mRNAs, miRNAs, lncRNAs) and their diagnostic and prognostic potential in cancer are outlined, ii) major challenges associated with current techniques are identified, followed by a critical analysis of how these challenges have been addressed by different biosensing approaches, and iii) the current requirements that still need to be met for effective screening of RNA biomarkers in both research and clinical settings.

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“…Abnormal expression of mRNAs has been widely demonstrated in human cancers, and researches suggested that the dysregulated mRNAs emerged as critical roles in various biological functions associated with progression of cancers. 27,28 However, only a few reports have identified mRNA profiles in T-cell lymphoma by microarray analysis. 16 In this study, we identified mRNAs in T-cell lymphomas from GEO database, which may be related to diagnosis, classification, medical treatment sensitivity, and tumor microenvironment in T-cell lymphomas.…”

Section: Discussionmentioning

confidence: 99%

T‐cell lymphomas associated gene expression signature: Bioinformatics analysis based on gene expression Omnibus

Zhou

et al. 2018

European J of Haematology

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Genome-wide analysis of microRNA and mRNA expression signatures in cancer

Cited by 65 publications

References 194 publications

Survival of Patients with Gastrointestinal Cancers Can Be Predicted by a Surrogate microRNA Signature for Cancer Stem–like Cells Marked by DCLK1 Kinase

Survival of Patients with Gastrointestinal Cancers Can Be Predicted by a Surrogate microRNA Signature for Cancer Stem–like Cells Marked by DCLK1 Kinase

RNA Biomarkers: Diagnostic and Prognostic Potentials and Recent Developments of Electrochemical Biosensors

T‐cell lymphomas associated gene expression signature: Bioinformatics analysis based on gene expression Omnibus

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