Genome-Wide Analysis of Multidrug and Toxic Compound Extrusion (MATE) Family inGossypium raimondiiandGossypium arboreumand Its Expression Analysis Under Salt, Cadmium, and Drought Stress

Abstract

The extrusion of toxins and substances at a cellular level is a vital life process in plants under abiotic stress. The multidrug and toxic compound extrusion (MATE) gene family plays a large role in the exportation of toxins and other substrates. We carried out a genome-wide analysis of MATE gene families in Gossypium raimondii and Gossypium arboreum and assessed their expression levels under salt, cadmium and drought stresses. We identified 70 and 68 MATE genes in G. raimondii and G. arboreum, respectively. The majority of the genes were predicted to be localized within the plasma membrane, with some distributed in other cell parts. Based on phylogenetic analysis, the genes were subdivided into three subfamilies, designated as M1, M2 and M3. Closely related members shared similar gene structures, and thus were highly conserved in nature and have mainly evolved through purifying selection. The genes were distributed in all chromosomes. Twenty-nine gene duplication events were detected, with segmental being the dominant type. GO annotation revealed a link to salt, drought and cadmium stresses. The genes exhibited differential expression, with GrMATE18, GrMATE34, GaMATE41 and GaMATE51 significantly upregulated under drought, salt and cadmium stress, and these could possibly be the candidate genes. Our results provide the first data on the genome-wide and functional characterization of MATE genes in diploid cotton, and are important for breeders of more stress-tolerant cotton genotypes.

Genetic regulatory networks for salt-alkali stress in Gossypium hirsutum with differing morphological characteristics

“…Although it is complicated to regulate alkali-salt stress in root, regulation of oxidation-reduction was performed in all stages. The results obtained were in agreement to previous findings in which similar GO terms have been identified for various stress responsive genes such as LEA genes [25], MATE genes [31] among others.…”

A Genome-Wide Survey of MATE Transporters in Brassicaceae and Unveiling Their Expression Profiles under Abiotic Stress in Rapeseed

“…Previous studies have localized MATE transporters to the plasma membrane, chloroplast, cytoplasm, vacuole, and endoplasmic reticulum, as well as extracellularly [ 24 , 38 , 39 ]. Here, we found the highest proportion localized in the plasma membrane (109 genes), accounting for >80% of all BnaMATE genes in B. napus ; four genes were expressed in chloroplasts and 11 genes were expressed in the vacuoles.…”

The Pepper Late Embryogenesis Abundant Protein, CaDIL1, Positively Regulates Drought Tolerance and ABA Signaling

“…LEA proteins function in a large spectrum of cellular processes, from growth to stress response ( Xiao et al, 2007 ; Campos et al, 2013 ; Pathak and Ikeno, 2017 ). In vascular plants, LEA proteins are highly expressed in embryos during the late embryogenesis stage and in vegetative organs under drought stress conditions, indicating that these proteins play an adaptive role in water limitation ( Dure and Chlan, 1981 ; Baker et al, 1988 ; Lu et al, 2018 ). Previous studies showed that some LEA proteins are involved in ABA signaling and protect cells against stresses, especially osmotic stress ( Hu et al, 2008 ; Candat et al, 2014 ; Lim et al, 2015c ).…”