Genome‐wide analysis of oral squamous cell carcinomas revealed over expression of ISG15, Nestin and WNT11

Vincent‐Chong, Vui King; Ismail, Siti Mazlipah; Rahman, Zulkanain Abdul; Na, Sharifah; Anwar, Arif; Pradeep, Padmaja Jayaprasad; Ramanathan, Anand; Karen-Ng, Lee Peng; Kallarakkal, Thomas George; Mustafa, Wan Mahadzir Wan; Abraham, Mannil Thomas; Tay, Keng Kiong; Zain, Rosnah Binti

doi:10.1111/j.1601-0825.2011.01894.x

Cited by 17 publications

(18 citation statements)

References 37 publications

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“…For various cancers, gene losses were frequently found in the short arm of chromosome arm 8p while most gene amplifications were found in 8q5354. The same pattern of gains and losses was also found in OSCCs781055. Our results were consistent with these studies.…”

Section: Discussionsupporting

confidence: 91%

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Genetic Alterations of Chromosome 8 Genes in Oral Cancer

Yong

Zaini

Kallarakkal

et al. 2014

Sci Rep

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The clinical relevance of DNA copy number alterations in chromosome 8 were investigated in oral cancers. The copy numbers of 30 selected genes in 33 OSCC patients were detected using the multiplex ligation-dependent probe amplification (MLPA) technique. Amplifications of the EIF3E gene were found in 27.3% of the patients, MYC in 18.2%, RECQL4 in 15.2% and MYBL1 in 12.1% of patients. The most frequent gene losses found were the GATA4 gene (24.2%), FGFR1 gene (24.2%), MSRA (21.2) and CSGALNACT1 (12.1%). The co-amplification of EIF3E and RECQL4 was found in 9% of patients and showed significant association with alcohol drinkers. There was a significant association between the amplification of EIF3E gene with non-betel quid chewers and the negative lymph node status. EIF3E amplifications did not show prognostic significance on survival. Our results suggest that EIF3E may have a role in the carcinogenesis of OSCC in non-betel quid chewers.

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Section: Discussionsupporting

confidence: 91%

“…Gains in chromosome 8q and losses in 8p are the common alterations found78910 and have been shown to be involved in the lymph node metastasis of OSCCs79. Located in chromosome 8 is the well-known gene, c-MYC (8q24), which is frequently amplified in various cancers111213.…”

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confidence: 99%

Genetic Alterations of Chromosome 8 Genes in Oral Cancer

Yong

Zaini

Kallarakkal

et al. 2014

Sci Rep

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“…Array-CGH was carried out using the SurePrint G3 Human CGH 1×1 M array (Agilent Technologies, Santa Clara, CA, USA) for genome wide survey according to the manufacturer’s protocol by Oxford Gene Technology as described previously [14]. The array slides used were oligonucleotide-based microarrays which containing 974,016 probes that enable molecular profiling of genomic imbalances with 2.1 kb average resolution.…”

Section: Methodsmentioning

confidence: 99%

Genome Wide Analysis of Chromosomal Alterations in Oral Squamous Cell Carcinomas Revealed over Expression of MGAM and ADAM9

et al. 2013

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Despite the advances in diagnosis and treatment of oral squamous cell carcinoma (OSCC), mortality and morbidity rates have not improved over the past decade. A major drawback in diagnosis and treatment of OSCC is the lack of knowledge relating to how genetic instability in oral cancer genomes affects oral carcinogenesis. Hence, the key aim of this study was to identify copy number alterations (CNAs) that may be cancer associated in OSCC using high-resolution array comparative genomic hybridization (aCGH). To our knowledge this is the first study to use ultra-high density aCGH microarrays to profile a large number of OSCC genomes (n = 46). The most frequently amplified CNAs were located on chromosome 11q11(52%), 2p22.3(52%), 1q21.3–q22(54%), 6p21.32(59%), 20p13(61%), 7q34(52% and 72%),8p11.23–p11.22(80%), 8q11.1–q24.4(54%), 9q13–q34.3(54%), 11q23.3–q25(57%); 14q21.3–q31.1(54%); 14q31.3–q32.33(57%), 20p13–p12.3(54%) and 20q11.21–q13.33(52%). The most frequently deleted chromosome region was located on 3q26.1 (54%). In order to verify the CNAs from aCGH using quantitative polymerase chain reaction (qPCR), the three top most amplified regions and their associated genes, namely ADAM5P (8p11.23–p11.22), MGAM (7q34) and SIRPB1 (20p13.1), were selected in this study. The ADAM5P locus was found to be amplified in 39 samples and deleted in one; MGAM (24 amplifications and 3 deletions); and SIRPB1 (12 amplifications, others undetermined). On the basis of putative cancer-related annotations, two genes, namely ADAM metallopeptidase domain 9 (ADAM9) and maltase-glucoamylase alpha-glucosidase (MGAM), that mapped to CNA regions were selected for further evaluation of their mRNA expression using reverse transcriptase qPCR. The over-expression of MGAM was confirmed with a 6.6 fold increase in expression at the mRNA level whereas the fold change in ADAM9 demonstrated a 1.6 fold increase. This study has identified significant regions in the OSCC genome that were amplified and resulted in consequent over-expression of the MGAM and ADAM9 genes that may be utilized as biological markers for OSCC.

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“…The binding of ISG15 to intracellular target proteins requires the sequential action of three enzymes, the expression of which are also induced by IFNs [19, 20]. Perturbations of the ISG15 pathway have been reported in tumors of the bladder [20], ovary [21], prostate [22], breast [23, 24], and oral squamous tissue [25], and the downregulation of ISG15 expression has been shown to be associated with tumor progression [20]. …”

Section: Introductionmentioning

confidence: 99%

ISG15 in the tumorigenesis and treatment of cancer: An emerging role in malignancies of the digestive system

Zuo

Sheng

et al. 2016

Oncotarget

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The interferon-stimulated gene 15 ubiquitin-like modifier (ISG15) encodes an IFN-inducible, ubiquitin-like protein. The ISG15 protein forms conjugates with numerous cellular proteins that are involved in a multitude of cellular functions, including interferon-induced immune responses and the regulation of cellular protein turnover. The expression of ISG15 and ISG15-mediated conjugation has been implicated in a wide range of human tumors and cancer cell lines, but the roles of ISG15 in tumorigenesis and responses to anticancer treatments remain largely unknown. In this review, we discuss the findings of recent studies with regard to the role of ISG15 pathways in cancers of the digestive system.

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Genome‐wide analysis of oral squamous cell carcinomas revealed over expression of ISG15, Nestin and WNT11

Abstract: This study has identified several major CNAs in oral cancer genomes and indicated that this correlates with over expression of the ISG15, WNT11, and Nestin genes.

Cited by 17 publications

References 37 publications

Genetic Alterations of Chromosome 8 Genes in Oral Cancer

Genetic Alterations of Chromosome 8 Genes in Oral Cancer

Genome Wide Analysis of Chromosomal Alterations in Oral Squamous Cell Carcinomas Revealed over Expression of MGAM and ADAM9

ISG15 in the tumorigenesis and treatment of cancer: An emerging role in malignancies of the digestive system

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