Genome-wide Analysis of Phosphoenolpyruvate Carboxylase Gene Family and Their Response to Abiotic Stresses in Soybean

Abstract

Phosphoenolpyruvate carboxylase (PEPC) plays an important role in assimilating atmospheric CO2 during C4 and crassulacean acid metabolism photosynthesis, and also participates in various non-photosynthetic processes, including fruit ripening, stomatal opening, supporting carbon–nitrogen interactions, seed formation and germination, and regulation of plant tolerance to stresses. However, a comprehensive analysis of PEPC family in Glycine max has not been reported. Here, a total of ten PEPC genes were identified in soybean and denominated as GmPEPC1-GmPEPC10. Based on the phylogenetic analysis of the PEPC proteins from 13 higher plant species including soybean, PEPC family could be classified into two subfamilies, which was further supported by analyses of their conserved motifs and gene structures. Nineteen cis-regulatory elements related to phytohormones, abiotic and biotic stresses were identified in the promoter regions of GmPEPC genes, indicating their roles in soybean development and stress responses. GmPEPC genes were expressed in various soybean tissues and most of them responded to the exogenously applied phytohormones. GmPEPC6, GmPEPC8 and GmPEPC9 were significantly induced by aluminum toxicity, cold, osmotic and salt stresses. In addition, the enzyme activities of soybean PEPCs were also up-regulated by these treatments, suggesting their potential roles in soybean response to abiotic stresses.

Nitrogen Fixation in Soybean Nodules Affects Seed Protein and Oil Contents: The Suggested Mechanism from the Coordinated Changes of Seed Chemical Compositions and Phosphoenolpyruvate Carboxylase Activity Caused by Different Types of Nitrogen Fertilizer

“…Another PEPC isogene expressed in soybean maturing seeds was identified together with the isogene mentioned above [24]. In recent, it appeared that ten Nitrogen Fixation in Soybean Nodules Affects Seed Protein and Oil Contents… DOI: http://dx.doi.org/10.5772/intechopen.96795 PEPC isogenes were encoded in the soybean genome [25]. It might be likely that multiple PEPC supports soybean seed metabolism during seed development.…”

Multi‐omic dissection of the drought resistance traits of soybean landrace LX

“…Exploration of the molecular mechanism of soybean drought tolerance and the development of drought-tolerant soybean varieties are two major themes of soybean breeding programs (Chen et al, 2018;L. S. Wang, Chen, et al, 2018;N. Wang et al, 2016).…”

“…Drought, often combined with salt stress, is a major factor limiting soybean germination, growth and yield. Exploration of the molecular mechanism of soybean drought tolerance and the development of drought‐tolerant soybean varieties are two major themes of soybean breeding programs (Chen et al, 2018; L. S. Wang, Chen, et al, 2018; N. Wang et al, 2016). These practices have often highlighted the particular significance of the biosynthesis of phenylalanine‐derived secondary metabolites following environmental stresses such as drought, cold, UV light exposure, insect damage or microbial infection (Davies et al, 2020).…”

Prokaryotic Expression of Phosphoenolpyruvate Carboxylase Fragments from Peanut and Analysis of Osmotic Stress Tolerance of Recombinant Strains

“…These storage compounds enter the tricarboxylic acid cycle (TCA) with intermediates such as pyruvate and phospho enol pyruvate (PEP) involve in a variety of biosynthetic pathways [ 1 , 9 ]. In addition to the photosynthesis, PEPC also plays important role in carbon–nitrogen interactions, maintaining cellular pH, seed formation and germination, fruit maturation, control of stomatal movements, supplying carbon for symbiotic N 2 -fixation in root nodules of leguminous plants, and regulates the mechanism of stress tolerance in plants [ 10 , 11 , 12 , 13 ].…”