Genome-wide analysis of respiratory burst oxidase homolog gene family in pea (Pisum sativum L.)

Abstract

Plant respiratory burst oxidase homologs (RBOHs) are key enzymes regulating superoxide production, which is important for plant development and responses to biotic and abiotic stresses. This study aimed to characterize the RBOH gene family in pea (Pisum sativum L.). Seven PsRBOH genes were identified in the pea genome and were phylogenetically clustered into five groups. Collinearity analyses of the RBOHs identified four pairs of orthologs between pea and soybean. The gene structure analysis showed that the number of exons ranged from 6 to 16. Amino acid sequence alignment, conserved domain, and conserved motif analyses showed that all seven PsRBOHs had typical features of plant RBOHs. The expression patterns of PsRBOH genes in different tissues provided suggested their roles in plant growth and organ development. In addition, the expression levels of PsRBOH genes under different abiotic stresses were analyzed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results demonstrated that PsRBOH genes exhibited unique stress-response characteristics, which allowed for functional diversity in response to different abiotic stresses. Furthermore, four PsRBOHs had a high probability of localization in the plasma membrane, and PsRBOH6 was localized to the plasma membrane and endoplasmic reticulum. The results of this study provide valuable information for further functional analysis of pea RBOH genes and their role in plant adaptation to climate-driven environmental constraints.

Genome wide comprehensive characterization and expression analysis of RBOH genes revealed drought stress response in mung bean (Vigna radiate L.)

“…For example, the VraRBOH1 gene was exhibited intronless gene structure. The intronless gene structure could be the species-speci c to Vigna spp as it was not reported in other legume crops yet (Liu et al 2019;Liu et al 2023). Even, in case of other plants, few plant species possessed the intronless gene structure in RBOH gene family (Hu et al 2018;Cheng et al 2020).…”

“…Interestingly, The VraRBOH4 was exponentially expressed in up-regulated manner during drought stress in the present study. In case of pea, the PsRBOH4 was also shown the similar pattern of expression during drought stress(Liu et al 2023). Similarly, the activated expression behaviour of RBOH genes in various studies in plants during droughtstress treatments (Cheng et al 2013; Wang et al 2016; Shi et al 2020; Du et al 2023; Sharma et al 2024) support the ndings obtained in the present study.…”

The Characteristics and Expression Analysis of the Tomato SlRBOH Gene Family under Exogenous Phytohormone Treatments and Abiotic Stresses

“…The tissue-specific expression of genes is essential for plant growth and development and provides important insights into understanding gene function [ 36 ]. Tissue-specific expression patterns of RBOHs have been reported in many plants.…”