Genome-Wide Analysis of the ATP-Binding Cassette (ABC) Transporter Family in Zea mays L. and Its Response to Heavy Metal Stresses

Abstract

The ATP-binding cassette (ABC) transporter family is one of the largest eukaryotic protein families. Its members play roles in numerous metabolic processes in plants by releasing energy for substrate transport across membranes through hydrolysis of ATP. Maize belongs to the monocotyledonous plant family, Gramineae, and is one of the most important food crops in the world. We constructed a phylogenetic tree with individual ABC genes from maize, rice, sorghum, Arabidopsis, and poplar. This revealed eight families, each containing ABC genes from both monocotyledonous and dicotyledonous plants, indicating that the amplification events of ABC gene families predate the divergence of plant monocotyledons. To further understand the functions of ABC genes in maize growth and development, we analyzed the expression patterns of maize ABC family genes in eight tissues and organs based on the transcriptome database on the Genevestigator website. We identified 133 ABC genes expressed in most of the eight tissues and organs examined, especially during root and leaf development. Furthermore, transcriptome analysis of ZmABC genes showed that exposure to metallic lead induced differential expression of many maize ABC genes, mainly including ZmABC 012, 013, 015, 031, 040, 043, 065, 078, 080, 085, 088, 102, 107, 111, 130 and 131 genes, etc. These results indicated that ZmABC genes play an important role in the response to heavy metal stress. The comprehensive analysis of this study provides a foundation for further studies into the roles of ABC genes in maize.

Comprehensive genome-wide identification and characterization of ABC Transporter superfamily in Cajanus cajan and its expression profiling under different developmental stages in various anatomical tissues

“…Similarly, the molecular weight of the CcABC proteins ranged from 6.97 to 345.38. The isoelectric point of the CcABC proteins lies in the range of 4.44 to 10.74 in the same manner as it has been reported in the ABC proteins of plants like Zea mays (Guo et al, 2022), 5.08 to 9.74 strawberry (Shi et al, 2020), 5.4 to 8.96 in cotton (Sun N et al 2021), 4.41 to 10.5 in soyabean (Mishra et al 2019) 5.05 to 10.48 in peach (Sylvia et al 2023)and tomato (Ofori PA et al 2018). In C. cajan, out of 159 CcABC proteins, 105 proteins were basic and 50 were acidic in nature, whereas 2 were neutral proteins.…”

“…All the motifs of ABC gene family were highly conserved as reported in peach (Sylvia C et al, 2023), brassica rapa (Yan C et al2017), zea mays (Guo Z et al 2022), soyabean (Huang J et al 2021) and capsicum (Ortiz C et al 2019). It were also mentioned in the pineapple (Chen P et al 2017), hexaploid wheat (Bhati KK et al 2015), barley (Zhang Z et al 2020), strawberry (M. Shi et al, 2020), and flax (Khan et al2020) that all the conserved motifs were identified.…”

Genomic-regions associated with cold stress tolerance in Asia-adapted tropical maize germplasm

“…Families of Serine/Threonine protein kinases (SNRK-2) are known to enhance cold stress tolerance in Arabidopsis 88 . In addition, enhanced expression of few of SNRK-2 genes have also been reported under cold stress in maize 89,90 . Further, WRKY transcription factors constitute one of the largest transcription factor families in plants and play important role in plant growth and thermal stress response 91 .…”

Transcriptome Analysis of the Late-Acting Self-Incompatibility Associated with RNase T2 Family in Camellia oleifera

“…ABC transporters can transport multiple types of substrates across the plasma membrane via ATP hydrolysis in response to environmental stress [ 56 , 57 ]. A previous study in M. domestica demonstrated that MdABCF in pollen could combine with S-RNase, a key protein of GSI, and transport it to the cytoplasm of the pollen tube to facilitate its cellular cytotoxic function [ 34 ].…”