Genome-Wide Analysis of the Auxin/Indoleacetic Acid Gene Family and Response to Indole-3-Acetic Acid Stress in Tartary Buckwheat (Fagopyrum tataricum)

Abstract

Auxin/indoleacetic acid (Aux/IAA) family genes respond to the hormone auxin, which have been implicated in the regulation of multiple biological processes. In this study, all 25 Aux/IAA family genes were identified in Tartary buckwheat (Fagopyrum tataricum) by a reiterative database search and manual annotation. Our study provided comprehensive information of Aux/IAA family genes in buckwheat, including gene structures, chromosome locations, phylogenetic relationships, and expression patterns. Aux/IAA family genes were nonuniformly distributed in the buckwheat chromosomes and divided into seven groups by phylogenetic analysis. Aux/IAA family genes maintained a certain correlation and a certain species-specificity through evolutionary analysis with Arabidopsis and other grain crops. In addition, all Aux/IAA genes showed a complex response pattern under treatment of indole-3-acetic acid (IAA). These results provide valuable reference information for dissecting function and molecular mechanism of Aux/IAA family genes in buckwheat.

Genome-Wide Analysis of Aux/IAA Gene Family in Artemisia argyi: Identification, Phylogenetic Analysis, and Determination of Response to Various Phytohormones

“…In our study, tissue expression analysis in roots and leaves in RNA-seq data demonstrated that the expression level of AaIAAs differed, such as AaIAA11, AaIAA19, AaIAA20, AaIAA30, AaIAA51, AaIAA58, and AaIAA61 genes being significantly higher in leaves than in roots, and 25 remaining members exhibited significantly higher levels in roots than that in leaves. These results indicated that more AaIAA members act on the roots and regulate plant growth and development, similar to findings in Fagopyrum tataricum, which also exhibited significantly lower expression levels of most FtAux/IAA genes in the leaves than in root tissues [47]. Previous studies have shown that Aux/IAA genes are involved in root growth and development, and OsIAA11 is expressed in the root tip, lateral root, middle column, and lateral root primordia [48].…”

“…Aux/IAA is critical, acting as a class of primary auxin-responsive genes, rapidly induced by auxin. In Fagopyrum tataricum , all Aux/IAA genes exhibited an intricate response pathway under IAA treatment; among them, different genes exhibited differing trends upon treatment for extended durations, and the expression of some genes differed in different tissues [ 47 ]. In our study, 12 AaIAA genes also showed a complex pattern in response to IAA treatment by RT-qPCR.…”

Serendipita indica Promotes the Growth of Tartary Buckwheat by Stimulating Hormone Synthesis, Metabolite Production, and Increasing Systemic Resistance

“…IAA is the main form of plant auxin and plays multiple roles in plant growth and development. It mediates the elongation of stem and root growth, the enlargement of fruits and tubers, and the promotion of cell division, through regulating cell division, expansion, differentiation, and patterning [34]. IAA is the best-studied naturally occurring active auxin.…”