Genome-wide analysis of the serine carboxypeptidase-like (SCPL) proteins in Brassica napus L.

Liu, Yilin; Ce, Fuquan; Tang, Huan; Tian, Guifu; Yang, Lei; Qian, Wei; Dong, Hongli

doi:10.1016/j.plaphy.2022.07.020

Cited by 11 publications

(6 citation statements)

References 51 publications

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“…The SCPL proteins contain a conserved catalytic triplet composed of three amino acid residues—a serine, an aspartate and a histidine (Ser‐Asp‐His) (Mugford and Milkowski, 2012)—that participates in transacylation reactions in plant secondary metabolism pathways (Weier et al ., 2008). Members of the SCPL class have been identified and characterized in wheat ( Triticum aestivum ) (Xu et al ., 2021), rapeseed ( Brassica napus ) (Liu et al ., 2022), grape ( Vitis vinifera ) (Wang et al ., 2021), cotton ( Gossypium hirsutum ) (Wang et al ., 2022) and tea ( Camellia sinensis ) (Ahmad et al ., 2020). Previous studies have ascertained that SCPLs play essential functions in determining seed weight and size (Dong et al ., 2023; Li et al ., 2011), signal transduction (Xu et al ., 2015), development and senescence of organs (Cercós et al ., 2003; Domínguez et al ., 2002), plant abiotic stress responses (Xu et al ., 2021) and plant disease resistance (Mugford et al ., 2009; Wang et al ., 2022).…”

Section: Discussionmentioning

confidence: 99%

A 5.2‐kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase‐like enhances fruit firmness in Prunus avium

Qi,

Dong,

Liu

et al. 2024

Plant Biotechnology Journal

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SummaryFruit firmness is an important trait in sweet cherry breeding because it directly positively influences fruit transportability, storage and shelf life. However, the underlying genes responsible and the molecular mechanisms that control fruit firmness remain unknown. In this study, we identified a candidate gene, PavSCPL, encoding a serine carboxypeptidase‐like protein with natural allelic variation, that controls fruit firmness in sweet cherry using map‐based cloning and functionally characterized PavSCPL during sweet cherry fruit softening. Genetic analysis revealed that fruit firmness in the ‘Rainier’ × ‘Summit’ F1 population was controlled by a single dominant gene. Bulked segregant analysis combined with fine mapping narrowed the candidate gene to a 473‐kb region (7418778–7 891 914 bp) on chromosome 6 which included 72 genes. The candidate gene PavSCPL, and a null allele harbouring a 5244‐bp insertion in the second exon that completely inactivated PavSCPL expression and resulted in the extra‐hard‐flesh phenotype, were identified by RNA‐sequencing analysis and gene cloning. Quantitative RT‐PCR analysis revealed that the PavSCPL expression level was increased with fruit softening. Virus‐induced gene silencing of PavSCPL enhanced fruit firmness and suppressed the activities of certain pectin‐degrading enzymes in the fruit. In addition, we developed functional molecular markers for PavSCPL and the Pavscpl5.2‐k allele that co‐segregated with the fruit firmness trait. Overall, this research identified a crucial functional gene for fruit firmness. The results provide insights into the genetic control and molecular mechanism of the fruit firmness trait and present useful molecular markers for molecular‐assisted breeding for fruit firmness in sweet cherry.

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Section: Discussionmentioning

confidence: 99%

A 5.2‐kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase‐like enhances fruit firmness in Prunus avium

Qi,

Dong,

Liu

et al. 2024

Plant Biotechnology Journal

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“…The serine carboxypeptidase-like genes ( SCPLs ) hold a significant position in plant growth, development, and responses to various stress [ 11 , 50 , 51 , 52 ]. Genome-wide investigation of SCPLs in multiple plants, including Arabidopsis thaliana , rice ( Oryza sativa ), bread wheat ( Triticum aestivum ), cotton ( Gossypium hirsutum ), rapeseed ( Brassica napus ), and the tea plant ( Camellia sinensis ), had been performed previously [ 19 , 20 , 22 , 53 , 54 ]. As one of the most important protein feed and vegetable oil crops for human consumption, cultivated soybean ( Glycine max ) originated from wild soybean ( Glycine soja ) in China about 5000 years ago [ 26 , 27 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Serine Carboxypeptidase-like Genes in Soybean and Their Roles in Stress Resistance

He,

Liu,

Han

2024

IJMS

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The serine carboxypeptidase-like (SCPL) gene family plays a crucial role in the regulation of plant growth, development, and stress response through activities such as acyltransferases in plant secondary metabolism pathways. Although SCPL genes have been identified in various plant species, their specific functions and characteristics in soybean (Glycine max) have not yet been studied. We identified and characterized 73 SCPL genes, grouped into three subgroups based on gene structure and phylogenetic relationships. These genes are distributed unevenly across 20 soybean chromosomes and show varied codon usage patterns influenced by both mutation and selection pressures. Gene ontology (GO) enrichment suggests these genes are involved in plant cell wall regulation and stress responses. Expression analysis in various tissues and under stress conditions, including the presence of numerous stress-related cis-acting elements, indicated that these genes have varied expression patterns. This suggests that they play specialized roles such as modulating plant defense mechanisms against nematode infections, enhancing tolerance to drought and high salinity, and responding to cold stress, thereby helping soybean adapt to environmental stresses. Moreover, the expression of specific GmSCPLs was significantly affected following exposure to nematode infection, drought, high salt (NaCl), and cold stresses. Our findings underscore the potential of SCPL genes in enhancing stress resistance in soybean, providing a valuable resource for future genetic improvement and breeding strategies.

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“…BOB01G136670 encodes a typical serine carboxypeptidase that belongs to the group I in the SCPL family [63,64]. The SCPL genes are widely present in higher plants, playing essential roles in plant stress tolerance, disease resistance, plant growth and especially in seed development [63,64].…”

Section: Discussionmentioning

confidence: 99%

Identification of Loci for Four Important Agronomic Traits in Loose-Curd Cauliflower Based on Genome-Wide Association Studies

Zhang,

Wen,

Jiang

et al. 2023

Horticulturae

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Cauliflower is a nutritious vegetable with inflorescences that are specialized to form the edible organs called curds. Uncovering key genes underlying important traits is crucial for the genetic improvement of this important crop. However, the genetic basis of many important agronomic traits, including curd performance and plant architecture in cauliflower, remains unclear. GWASs have proved to be powerful tools to study agronomic traits in many crops. To reveal the genetic basis of four important agronomic traits, namely, the main stem height (MSH), purplish curd (PC), external leaf wing (ELW) and weight of a single curd (WSC), we selected 220 core accessions of loose-curd cauliflower for resequencing, phenotypic investigation and GWAS. The approach revealed significant novel loci. We detected several significant associations: on C02 for MSH and PC, on C06 for ELW and on C01 for WSC. More interestingly, we identified a significant single-peak signal for the weight of a single curd (WSC), an important yield trait, and within this signal interval, we identified the BOB01G136670 gene with five SNPs encoding nonsynonymous mutations in the CDS region; these mutations resulted in two haplotypes with significant differences in curd weight. The weight of a single curd was significantly increased in the varieties with the BOB01G136670 Hap1 allele compared to those with BOB01G136670 Hap2. BOB01G136670 was highly conserved with the homologous genes that encode serine carboxypeptidase and belong to the S10 family in other species, including GS5, which functions as a positive regulator of grain size in rice, wheat and maize. Additionally, BOB01G136670 was highly expressed specifically at the curd enlargement stage, with low or even no expression at all in other tissues and stages, indicating that BOB01G136670 is a plausible candidate gene for WSC. Overall, this study identified genomic loci for four important agronomic traits that are relevant for accelerating biological breeding and the improvement of cauliflower varieties.

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Genome-wide analysis of the serine carboxypeptidase-like (SCPL) proteins in Brassica napus L.

Cited by 11 publications

References 51 publications

A 5.2‐kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase‐like enhances fruit firmness in Prunus avium

A 5.2‐kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase‐like enhances fruit firmness in Prunus avium

Genome-Wide Analysis of Serine Carboxypeptidase-like Genes in Soybean and Their Roles in Stress Resistance

Identification of Loci for Four Important Agronomic Traits in Loose-Curd Cauliflower Based on Genome-Wide Association Studies

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