Genome-wide analysis of thiourea-modulated salinity stress-responsive transcripts in seeds of Brassica juncea: identification of signalling and effector components of stress tolerance

Srivastava, Ashish Kumar; Ramaswamy, N.K.; Suprasanna, Penna; D’Souza, S.F.

doi:10.1093/aob/mcq163

Cited by 48 publications

(31 citation statements)

References 43 publications

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“…This shows the antioxidant activity of these exogenously applied organic compounds in scavenging ROS. These findings of the present study are analogous to some previous studies in which it was reported that foliar applications of mannitol and thiourea are effective in reducing the adverse effects of oxidative stress on biological membranes by acting as a scavenger of ROS (Mitoi et al 2009;Srivastava et al 2010). …”

Section: Discussionsupporting

confidence: 92%

“…Of them, thiourea plays a key role in oxidative stress response. Recently, Srivastava et al (2010) have reported that thiourea treatment can alleviate the adverse effects of salt stress on Brassica juncea seeds by coordinately bioregulating different signaling and effecter mechanisms.…”

Section: Introductionmentioning

confidence: 99%

“…Its proportion is about 50% of the total translocated photoassimilates (Loester et al 1992). Although mannitol plays an important role in osmotic adjustment, it acts as an antioxidant to scavenge of hydroxyl radicals (OH ) (Shen et al 1997;Srivastava et al 2010). However, there is little information available in the literature on the role of mannitol in stress tolerance in plants of agronomic importance.…”

Section: Introductionmentioning

confidence: 99%

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Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea maysL.)

Kaya

Sönmez

Aydemir

et al. 2013

Journal of Plant Interactions

103

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The mechanism of growth amelioration in salt-stressed maize (Zea mays L. cv., DK 647 F1) by exogenously applied mannitol (M) and thiourea (T) was investigated. Maize seedlings were planted in pots containing perlite and subjected to 0 or 100 mM NaCl in full strength Hoagland's nutrient solution. Two levels of M (15 and 30 mM) or T (3.5 and 7.0 mM) were sprayed to the leaves of maize seedlings 10 days after germination. Salinity stress caused considerable reduction in plant dry biomass, chlorophyll content, and relative water content in the maize plants. However, it increased the activities of catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1), and polyphenol oxidase (PPO; EC 1.10.3.1), and levels of hydrogen peroxide (H 2 O 2 ) and electrolyte leakage, but it did not change peroxidase (POD; EC 1.11.1.7) activity. Foliar application of M or T was found to be effective in checking salt-induced shoot growth inhibition. Exogenously applied M or T reduced the activities of CAT, SOD, POD, and PPO in the salt-treated maize plants compared to those in the plants not fed with these organic compounds. Salinity increased Na ' contents but decreased those of K ' , Ca 2 ' , and P in the leaves and roots of the maize plants. Foliar-applied M or T increased the contents of K ' , Ca 2 ' , and P, but decreased that of Na ' in the salt-stressed maize plants with respect to those of the salt-stressed plants not supplied with mannitol or thiourea. Mannitol was found to be more effective than thiourea in improving salinity tolerance of maize plants in terms of growth and physiological attributes measured in the present study.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea maysL.)

Kaya

Sönmez

Aydemir

et al. 2013

Journal of Plant Interactions

103

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“…The sequence of the gene-specific primer sets were GCCGGAG-TATCGGTCCTTTTTCG-F and AGCCGCTCTGAT-CACTTCAATCTG-R for phenyl-alanine ammonia lyase (pal) and ACCTGCAGGCATCTTGGCTATTGG-F and ACTGCCGCTTCTTTGCCTAGCTT-R for chalcone synthase (chs). The RNA isolation and quantitative real-time PCR were performed as per the method described previously (Srivastava et al 2010b). …”

Section: Estimation Of Proline Contentmentioning

confidence: 99%

Thiourea mediates alleviation of UV-B stress-induced damage in the Indian mustard (Brassica junceaL.)

Pandey

Srivastava

Suprasanna

et al. 2012

Journal of Plant Interactions

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UV-B radiation (280Á320 nm) as an environmental stress has damaging effect on plants and its overexposure can potentially interfere with growth and development. The effect of thiourea (TU) on UV-B stress tolerance of 10-day-old Brassica juncea seedlings subjected to supplementary UV-B for 5 days was investigated. An increase in the UV-B absorbing compounds anthocyanin, flavonoids and phenolic compounds was observed in UV-B'TU treated seedlings as compared to that of control. The enhanced synthesis of UV-B screening compounds resulted in lesser damage to chlorophyll and also gain in fresh weight and dry weight in UV-B'TU as compared to UV-B alone treatment. The enzymatic activities of guaiacol peroxidase (GPX) and superoxide dismutase (SOD) also increased in UV-B'TU. The expression profiling of phenylalanine ammonia lyase (pal) and chalcone synthase (chs) indicated an upregulation under UV-B'TU treatment, compared to that of control, suggesting that TU treatment ensured an early and efficient induction of flavonoid biosynthetic pathway. The results indicate that TU helps in ameliorating the damaging effects of UV-B stress by efficiently maintaining the antioxidant status and attenuating the penetration of the UV-B.

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“…In this situation, use of growth substances provides a short-term solution to overcome environmental stresses. In recent times, attention has been diverted to the improvement of crops for stress tolerance by enhanced biosynthesis or exogenous application of growth substances (Naidu and Williams, 2004;Quan et al, 2004) Srivastava et al (2010) while working with Brassica juncea have shown that exogenously applied thiourea can effectively alleviate the inhibitory effects of saline stress on B. juncea seeds by regulating signaling mechanisms. However, the effectiveness of thiourea and related growth regulators has been reported to depend on plant species, environmental conditions, and concentration of soil salt solution.…”

Section: Introductionmentioning

confidence: 99%

Exogenous application of nitric oxide and thiourea regulates on growth and some key physiological processes in maize (Zea mays L.) plants under saline stress.

Kaya¹,

Sönmez²,

Ashraf³

et al. 2015

Toprak Su Dergi̇si̇

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Effect of exogenously applied nitric oxide (NO) and thiourea (TU) in combination was examined in maize plants under saline stress. Seedlings of two maize cultivars (DK 5783 and Apex 836) were grown in pots containing soil salinized with 0 or 100 mM NaCl dissolved in irrigation water. Two levels of NO + TU (3 + 400 or 6 + 500 mg/L) were applied as presowing seed treatment or through leaves of 10-day old maize seedlings. Saline stress significantly suppressed plant fresh biomass, leaf water potential and chlorophyll content, but increased electrolyte leakage in both cultivars. However, these reductions were higher in Apex 836 than those in Dk 5783. Both treatments of combined NO and TU as seed soaking or foliar application were effective in mitigating the adverse effects of saline stress on shoot growth. Seed treatments of both levels of combined NO and TU were more effective in terms of improvement in fresh weights of DK 5783 than foliar treatments. Leaf Na + contents increased whereas those of N and P decreased in maize plants under saline regime. Application of Both modes of treatment of combined NO and TU increased the contents of N and P, but decreased that NO and TU through both modes increased Na+ in salt stressed maize plants. The results of the present study indicate that application of NO and TU compounds in combination alleviated the detrimental effects of salinity and increased resistance to salinity in the maize plants by improving plant growth

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Genome-wide analysis of thiourea-modulated salinity stress-responsive transcripts in seeds of Brassica juncea: identification of signalling and effector components of stress tolerance

Cited by 48 publications

References 43 publications

Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea maysL.)

Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea maysL.)

Thiourea mediates alleviation of UV-B stress-induced damage in the Indian mustard (Brassica junceaL.)

Exogenous application of nitric oxide and thiourea regulates on growth and some key physiological processes in maize (Zea mays L.) plants under saline stress.

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