Genome-Wide Analysis Reveals the Potential Role of MYB Transcription Factors in Floral Scent Formation in Hedychium coronarium

Abbas, Farhat; Ke, Yanguo; Zhou, Yiwei; Yu, Yunyi; Waseem, Muhammad; Ashraf, Umair; Wang, Chutian; Wang, Xiaoyu; Li, Xinyue; Yue, Yuechong; Yu, Rangcai; Fan, Yanting

doi:10.3389/fpls.2021.623742

Cited by 61 publications

(59 citation statements)

References 113 publications

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“…The transcriptional regulatory network governing floral scent emission has not been thoroughly elucidated. To date, only a few TFs that regulate the expression of scent-related genes have been identified ( Katiyar et al, 2012 ; Abbas et al, 2021b ). R2R3-MYB TFs are key regulators of the phenylpropanoid and terpenoids biosynthetic pathway in plants ( Du et al, 2009 ; Zhu et al, 2015 ; Yang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Auxin-Responsive R2R3-MYB Transcription Factors HcMYB1 and HcMYB2 Activate Volatile Biosynthesis in Hedychium coronarium Flowers

Abbas

Zhou

et al. 2021

Front. Plant Sci.

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Auxin, an important plant hormone, induces the biosynthesis of various secondary metabolites by modulating the expression of auxin-responsive genes. In the ornamental plant Hedychium coronarium, linalool and methyl benzoate are biosynthesized by the terpene synthase (TPS) HcTPS5 and the benzoic/salicylic acid methyltransferase (BSMT) HcBSMT2, respectively. However, the transcriptional regulation of this process remains unclear. Here, we identified and functionally characterized the R2R3-MYB transcription factors HcMYB1 and HcMYB2 in regulating the biosynthesis of these floral aroma compounds. HcMYB1 and HcMYB2 are specifically expressed in flowers, their expression is correlated with the emission of volatile compounds in flowers, and is induced by auxin. Moreover, HcMYB1 and HcMYB2 interact with the HcBSMT2 promoter region. HcMYB2 activates the expression of the linalool synthase gene HcTPS5. In flowers with HcMYB1 or HcMYB2 silenced, the levels of floral scent compounds were significantly reduced, and HcBSMT2 and HcTPS5 were downregulated compared with the wild type. Moreover, HcMYB1 form protein-protein interaction with key scent-related HcIAA4 protein to regulate floral aroma production. Taken together, these results indicate that HcMYB1 and HcMYB2 play crucial roles in regulating the formation of scent compounds in Hedychium coronarium (H. coronarium) flowers in response to auxin signaling.

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Section: Discussionmentioning

confidence: 99%

Auxin-Responsive R2R3-MYB Transcription Factors HcMYB1 and HcMYB2 Activate Volatile Biosynthesis in Hedychium coronarium Flowers

Abbas

Zhou

et al. 2021

Front. Plant Sci.

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“…After this, the bottle was immediately closed with aluminum foil. After 30 min, polydimethylsiloxane (PDMS, with 50/30 µm divinylbenzene/Carboxen, Supelco) fiber was injected into the bottle for 30 min to trap the volatiles, which was followed by insertion into a GC–MS (Agilent) or E-nose system for volatile analysis, as described previously [ 11 , 43 ]. The HS-SPME extraction was performed at 26 ± 2 °C.…”

Section: Methodsmentioning

confidence: 99%

“…With respect to their floral scent, Hedychium species vary from scentless to rich in fragrance with high ornamental values [ 6 , 7 , 8 ]. The flowers of Hedychium are rich in an aroma that is mainly composed of a mixture of monoterpenoids, sesquiterpenoids, and benzenoids [ 9 , 10 , 11 , 12 ]. Recently, the main focus has been on the physio-biochemical properties of Hedychium ; however, there are few reports on the qualitative and quantitative analysis of the floral volatile profiles of Hedychium .…”

Section: Introductionmentioning

confidence: 99%

HS–SPME–GC–MS and Electronic Nose Reveal Differences in the Volatile Profiles of Hedychium Flowers

et al. 2021

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Floral fragrance is one of the most important characteristics of ornamental plants and plays a pivotal role in plant lifespan such as pollinator attraction, pest repelling, and protection against abiotic and biotic stresses. However, the precise determination of floral fragrance is limited. In the present study, the floral volatile compounds of six Hedychium accessions exhibiting from faint to highly fragrant were comparatively analyzed via gas chromatography–mass spectrometry (GC–MS) and Electronic nose (E-nose). A total of 42 volatile compounds were identified through GC–MS analysis, including monoterpenoids (18 compounds), sesquiterpenoids (12), benzenoids/phenylpropanoids (8), fatty acid derivatives (2), and others (2). In Hedychium coronarium ‘ZS’, H. forrestii ‘Gaoling’, H. ‘Jin’, H. ‘Caixia’, and H. ‘Zhaoxia’, monoterpenoids were abundant, while sesquiterpenoids were found in large quantities in H. coccineum ‘KMH’. Hierarchical clustering analysis (HCA) divided the 42 volatile compounds into four different groups (I, II, III, IV), and Spearman correlation analysis showed these compounds to have different degrees of correlation. The E-nose was able to group the different accessions in the principal component analysis (PCA) corresponding to scent intensity. Furthermore, the pattern-recognition findings confirmed that the E-nose data validated the GC–MS results. The partial least squares (PLS) analysis between floral volatile compounds and sensors suggested that specific sensors were highly sensitive to terpenoids. In short, the E-nose is proficient in discriminating Hedychium accessions of different volatile profiles in both quantitative and qualitative aspects, offering an accurate and rapid reference technique for future applications.

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“…White ginger lily (H. coronarium) is famous due to its pure white color and butterfly flower shape. The H. coronarium flower emits a strong aroma, which is a combination of several floral volatiles including terpenes, benzenoids, and phenylpropanoids [1][2][3][4][5]. Monoterpenes and sesquiterpenes are the major floral volatile contents of this breed, and in our previous studies we identified several key volatile synthesis genes (HcTPS1/2/3/5/7/8/10, HcBSMT1/2, HcIAA2/4, HcARF5 and HcPAL) involved in floral aroma biosynthesis [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Hedychium coronarium J. Koenig MYB132 Confers the Potential Role in Floral Aroma Synthesis

Abbas

Zhou

et al. 2021

Plants

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The R2R3-MYB transcription factors (TFs) play several key roles in numerous plant biological processes. Hedychium coronarium is an important ornamental plant well-known for its elegant flower shape and abundant aroma type. The floral aroma of H. coronarium is due to the presence of a large amount of terpenes and benzenoids. However, less is known about the role of R2R3-MYB TFs in the regulatory mechanism of floral aroma production in this breed. Herein, we isolate and functionally characterize the R2R3-MYB TF HcMYB132, which is potentially involved in regulating floral aroma synthesis. Sequence alignment analysis revealed that it includes a nuclear localization signal NLS(s) and a 2R, 3R motif signature in the sequences. A subcellular localization assay revealed that HcMYB132 protein localizes to the nucleus. Real-time qPCR assays showed that HcMYB132 is specifically expressed in flowers and its expression pattern correlates with the emission of floral volatile compounds. In HcMYB132-silenced flowers, the levels of floral volatile compounds were significantly reduced, and the expression of key structural volatile synthesis genes was downregulated compared to control. Collectively, these results suggest that HcMYB132 might play a significant role in the regulation of terpenoid biosynthesis in H. coronarium.

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Genome-Wide Analysis Reveals the Potential Role of MYB Transcription Factors in Floral Scent Formation in Hedychium coronarium

Cited by 61 publications

References 113 publications

Auxin-Responsive R2R3-MYB Transcription Factors HcMYB1 and HcMYB2 Activate Volatile Biosynthesis in Hedychium coronarium Flowers

Auxin-Responsive R2R3-MYB Transcription Factors HcMYB1 and HcMYB2 Activate Volatile Biosynthesis in Hedychium coronarium Flowers

HS–SPME–GC–MS and Electronic Nose Reveal Differences in the Volatile Profiles of Hedychium Flowers

Functional Characterization of Hedychium coronarium J. Koenig MYB132 Confers the Potential Role in Floral Aroma Synthesis

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