Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis

Abstract

Background: The protein phosphatase 2Cs (PP2Cs) from various organisms have been implicated to act as negative modulators of protein kinase pathways involved in diverse environmental stress responses and developmental processes. A genome-wide overview of the PP2C gene family in plants is not yet available.

Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes

“…A genome-wide and expression analysis of clade A PP2Cs in rice and Arabidopsis indicates that most of them were induced by ABA and drought stress or mannitol treatment, and the ABA response-related element (ABRE) motif was significantly enriched in the promoter region of them (Xue et al, 2008;Singh et al, 2010). Overexpression of the rice clade A PP2C gene OsPP2C108 in Arabidopsis enhanced the tolerance to salt, mannitol, and drought stresses, and sensitivity to ABA treatment (Singh et al, 2015).…”

“…The rice genome contains 12 OsPYLs (Kim et al, 2012;He et al, 2014), 10 clade A PP2Cs (Xue et al, 2008), and 10 osmotic stress/ABA-activated protein kinases (SAPKs; Kobayashi et al, 2004). The molecular functions and interactions between some of these components were investigated in recent years.…”

Differentially regulated kinases and phosphatases in roots may contribute to inter-cultivar difference in rice salinity tolerance

“…10 Protein phosphatase 2C (PP2C) type phosphatases function as regulators of various signal transduction pathways. 11 The best characterised PP2Cs are ABI1/ABI2 and related proteins that are central components in abscisic acid (ABA) signal transduction. 12 Interestingly, this transcript is moderately upregulated in IR (two-fold) but strongly downregulated in FL roots (five-fold).…”

Structural insights into ABA perception and signalling: structure of ABA receptor PYR1

“…Moreover, these OsPP2Cs seem to also share roles with AtPP2CAs since they appear to be up-regulated in response to ABA and salt, similarly as AtPP2CAs (Xue et al, 2008), suggesting that OsPP2Cs might also be involved in ABA signaling. When looking for PYR/PYL/RCAR homologues in rice, 14 high identity genes popped up ( Figure 1B).…”

“…The overexpression approach of some of these elements, particularly of OsbZIP23 and OsbZIP72, has already been documented resulting in plants more tolerant to salinity and drought (Lu et al 2009;Xiang et al 2008). In the case of the OsPP2Cs, a comparative genome-wide overview of the PP2C family of Arabidopsis and rice, has revealed that both species have a similar number of genes encoding for PP2C proteins, as well as a similar number of PP2Cs classified as clade A, 9 and 10 respectively (Xue et al, 2008) ( Figure 1A).…”