Genome-Wide Annotation of Remorins, a Plant-Specific Protein Family: Evolutionary and Functional Perspectives

Raffaele, Sylvain; Mongrand, Sébastien; Gamas, Pascal; Niebel, Andréas; Ott, Thomas

doi:10.1104/pp.107.108639

Cited by 144 publications

(193 citation statements)

References 39 publications

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“…S2) are known to play roles in responses to environmental stress. These include homeoboxLeu zipper genes (Manavella et al, 2006; hormone data set), d-1-pyrroline-5-carboxylate synthase involved in Pro biosynthesis (Yoshiba et al, 1995; hormone data set), remorins (Raffaele et al, 2007; stress data set), potassium transporter family members (Szczerba et al, 2009; stress data set), cold-responsive genes (cor15a; Baker et al, 1994; hormone data set), and the RD26 gene encoding a NAC (for NAM [no apical meristem]ATAF [Arabidopsis Transcription Factor], and CUC [cup-shaped cotyledon]) transcription factor involved in drought and ABA signaling (Fujita et al, 2004; hormone and pathogen data sets). Thus, these observed coexpression patterns provide independent support in favor of a key function of RD20 in stress responses.…”

Section: Resultsmentioning

confidence: 99%

The Reductase Activity of the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20 Mediates Gibberellin-Dependent Flowering Time, Abscisic Acid Sensitivity, and Tolerance to Oxidative Stress

et al. 2014

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Contrasting with the wealth of information available on the multiple roles of jasmonates in plant development and defense, knowledge about the functions and the biosynthesis of hydroxylated oxylipins remains scarce. By expressing the caleosin RESPONSIVE TO DESSICATION20 (RD20) in Saccharomyces cerevisiae, we show that the recombinant protein possesses an unusual peroxygenase activity with restricted specificity toward hydroperoxides of unsaturated fatty acid. Accordingly, Arabidopsis (Arabidopsis thaliana) plants overexpressing RD20 accumulate the product 13-hydroxy-9,11,15-octadecatrienoic acid, a linolenate-derived hydroxide. These plants exhibit elevated levels of reactive oxygen species (ROS) associated with early gibberellin-dependent flowering and abscisic acid hypersensitivity at seed germination. These phenotypes are dependent on the presence of active RD20, since they are abolished in the rd20 null mutant and in lines overexpressing RD20, in which peroxygenase was inactivated by a point mutation of a catalytic histidine residue. RD20 also confers tolerance against stress induced by Paraquat, Rose Bengal, heavy metal, and the synthetic auxins 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid. Under oxidative stress, 13-hydroxy-9,11,15-octadecatrienoic acid still accumulates in RD20-overexpressing lines, but this lipid oxidation is associated with reduced ROS levels, minor cell death, and delayed floral transition. A model is discussed where the interplay between fatty acid hydroxides generated by RD20 and ROS is counteracted by ethylene during development in unstressed environments.

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Section: Resultsmentioning

confidence: 99%

The Reductase Activity of the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20 Mediates Gibberellin-Dependent Flowering Time, Abscisic Acid Sensitivity, and Tolerance to Oxidative Stress

et al. 2014

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“…The gene encodes a 531-amino acid protein with a predicted molecular mass of 58.26 kD and a pI of 8.94. Like other remorins that have been described (Raffaele et al, 2007), GSD1 protein contains a coiled-coil domain in the conserved C-terminal (Remorin_C), and nonconserved N-terminal residues (Remorin_N; Fig. 3B; Supplemental Fig.…”

Section: Gsd1 Encodes a Function-unknown Remorin Proteinmentioning

confidence: 99%

“…S10; Supplemental Table S1). GSD1, which belongs to remorin group 6, encodes a protein named remorin 6.6 following nomenclature used previously (Raffaele et al, 2007). Phylogenetically, GSD1 is close to Arabidopsis (Arabidopsis thaliana) AtREM6.2, whose function is still unknown (Supplemental Fig.…”

Section: Gsd1 Encodes a Function-unknown Remorin Proteinmentioning

confidence: 99%

“…S10 and S11A). Unlike other groups, group 6 remorins contain a long and highly variable N-terminal sequence ranging from 240 to 522 amino acids (Raffaele et al, 2007). The nonconserved N-terminal residues, which are predicted to be intrinsically disordered ) with a three-dimensional structure that varies depending on physiological conditions (Dyson and Wright, 2005), are believed to play an important role in regulation, signaling, and control through high-specificity, low-affinity protein-protein interactions (Sickmeier et al, 2007).…”

Section: Regulation Of Gsd1 Expression May Affect the Distribution Ofmentioning

confidence: 99%

“…Remorins can be classified into six distinct phylogenetic groups (Raffaele et al, 2007). The functions of most remorins are unknown, but some members of the family have been shown to be involved in immune response through controlling the cell-to-cell spread of microbes.…”

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Grain setting defect1, Encoding a Remorin Protein, Affects the Grain Setting in Rice through Regulating Plasmodesmatal Conductance

et al. 2014

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Effective grain filling is one of the key determinants of grain setting in rice (Oryza sativa). Grain setting defect1 (GSD1), which encodes a putative remorin protein, was found to affect grain setting in rice. Investigation of the phenotype of a transfer DNA insertion mutant (gsd1-Dominant) with enhanced GSD1 expression revealed abnormalities including a reduced grain setting rate, accumulation of carbohydrates in leaves, and lower soluble sugar content in the phloem exudates. GSD1 was found to be specifically expressed in the plasma membrane and plasmodesmata (PD) of phloem companion cells. Experimental evidence suggests that the phenotype of the gsd1-Dominant mutant is caused by defects in the grain-filling process as a result of the impaired transport of carbohydrates from the photosynthetic site to the phloem. GSD1 functioned in affecting PD conductance by interacting with rice ACTIN1 in association with the PD callose binding protein1. Together, our results suggest that GSD1 may play a role in regulating photoassimilate translocation through the symplastic pathway to impact grain setting in rice.

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Novel approach to measure the size of plasma‐membrane nanodomains in single molecule localization microscopy

et al. 2015

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Many membrane proteins are not evenly distributed over the plasma membrane, but gathered in domains assumed to have a particular lipid composition. Using single molecule localization microscopy (SMLM) we have immunolocalized a glycosylphosphatidylinositol (GPI)-anchor protein that labels nanodomains in a specialized plant cell type, and compared the suitability of three methods to estimate their size. As conventional methods full width at half maximum (FWHM) and the full diameter (FWMin) of domains were used. A boundary detection method of the domain area (DA) was performed in order to take irregular shapes into account. In order to compare the influence of the chosen measurement methods, we have developed a MatLab program that allows for automated analysis of domain sizes from multiple SMLM images and provides the statistics of three key features of domains: FWHM and FWMin along their long and short axes as well as the DA, derived from the molecular density. Domains formed by the GPI-anchor protein are approximating elliptical shapes. Direct and indirect immunolabeling resulted in a statistically significant difference in apparent domain size, reflecting the fact that the secondary antibody molecules extend the uncertainty along the nanodomain border. FWMin values along the long and short axis give good estimates of regular, geometrically centred domain shapes, while the DA value matches regular as well as irregular shapes best, as derived from computer-generated, irregular point clusters. V C 2015 International Society for Advancement of Cytometry

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Genome-Wide Annotation of Remorins, a Plant-Specific Protein Family: Evolutionary and Functional Perspectives

Cited by 144 publications

References 39 publications

The Reductase Activity of the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20 Mediates Gibberellin-Dependent Flowering Time, Abscisic Acid Sensitivity, and Tolerance to Oxidative Stress

The Reductase Activity of the Arabidopsis Caleosin RESPONSIVE TO DESSICATION20 Mediates Gibberellin-Dependent Flowering Time, Abscisic Acid Sensitivity, and Tolerance to Oxidative Stress

Grain setting defect1, Encoding a Remorin Protein, Affects the Grain Setting in Rice through Regulating Plasmodesmatal Conductance

Novel approach to measure the size of plasma‐membrane nanodomains in single molecule localization microscopy

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