Genome-wide association for β-glucan content, population structure, and linkage disequilibrium in elite oat germplasm adapted to subtropical environments

Zimmer, Cristiano Mathias; McNish, Ian; Klos, Kathy Esvelt; Oro, Tatiana; Arruda, Klever Márcio Antunes; Gutkoski, Luiz Carlos; Pacheco, Marcelo Teixeira; Smith, Kevin P.; Federizzi, Luiz Carlos

doi:10.1007/s11032-020-01182-0

Cited by 14 publications

(26 citation statements)

References 41 publications

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“…In total, 406 genotypes including commercial cultivars and inbred lines were assessed in this study. These genotypes are part of the Federal University of Rio Grande do Sul (UFRGS) Oat Panel, which was assembled with priority given to maximizing the differences among pedigrees (Zimmer et al., 2020). The UFRGS Oat Panel originally comprised 413 genotypes from different oat breeding programs, with 384 lines being hulled and 29 lines being naked.…”

Section: Methodsmentioning

confidence: 99%

“…(2020). The enzymatic method was performed with the mixed‐linkage β‐glucan assay kit (Megazyme International Ireland Ltd., Wicklow, Ireland), whereas NIRS predictions were performed with a NIRS DS2500 (Foss) analyzer (Zimmer et al., 2020). The equipment was calibrated with a small cup and 15 g of oat flour per sample ( n = 331).…”

Section: Methodsmentioning

confidence: 99%

“…(2012), as described by Zimmer et al. (2020). Briefly, libraries were sequenced with a NovaSeq 6000 sequencer (Illumina), producing individual 100‐bp single‐end reads.…”

Section: Methodsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

“…Samples screened for kernel shape traits in Londrina and Eldorado do Sul were also assessed for β-glucan content via enzymatic and near-infrared spectroscopy (NIRS) prediction, respectively, as described by Zimmer et al (2020). The enzymatic method was performed with the mixed-linkage β-glucan assay kit (Megazyme International Ireland Ltd., Wicklow, Ireland), whereas NIRS predictions were performed with a NIRS DS2500 (Foss) analyzer (Zimmer et al, 2020). The equipment was calibrated with a small cup and 15 g of oat flour per sample (n = 331).…”

Section: Phenotypic Assessmentmentioning

confidence: 99%

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Genome‐wide association mapping for kernel shape and its association with β‐glucan content in oats

et al. 2021

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Kernel shape and β‐glucan content are important attributes for oat (Avena sativa L.) millers. The identification of genomic regions controlling these traits, as well as their effect on phenotype, will enable rapid gain from selection. The objectives of this study were (a) to identify the genomic regions associated with kernel shape in an oat panel adapted to subtropical environments and (b) to clarify the relationship between kernel shape and β‐glucan content in oat. An elite oat panel comprising 406 inbred lines was assessed in Londrina and Eldorado do Sul, Southern Brazil. Genome‐wide association analyses were performed for each environment separately and also in a multi‐environment analysis. Phenotypic and genetic correlation coefficients were estimated between kernel shape traits and β‐glucan content. Genomic regions influencing kernel length were identified on Mrg06, Mrg21, and Mrg24. Kernel width and kernel thickness were genetically correlated and shared a quantitative trait locus, located on Mrg13 of the oat consensus map. Phenotypic and genetic correlations were found between kernel shape traits and β‐glucan content, indicating a negative relationship between kernel width or kernel thickness and β‐glucan content. Oat breeding programs that aim to maintain minimum levels of β‐glucan for nutrition and minimum kernel widths for milling will need to screen for both traits; otherwise, indirect selection will lead to unfavorable results. However, the negative correlation was not too strong (–0.4), suggesting that improvement for both traits can be made simultaneously.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…(2012), as described by Zimmer et al. (2020). Briefly, libraries were sequenced with a NovaSeq 6000 sequencer (Illumina), producing individual 100‐bp single‐end reads.…”

Section: Methodsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Phenotypic Assessmentmentioning

confidence: 99%

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