Genome-wide association identifies impacts of chlorophyll levels on reproductive maturity and architecture in maize

Abstract

The semi-dominant mutant allele Oy1-N1989 encodes a dominant-negative subunit I of Mg-Chelatase that catalyzes the first committed step of chlorophyll biosynthesis. Analyses of mutant F1 hybrids from crosses of Oy1-N1989 to diverse maize lines identified a natural variant at oil yellow1 as major modifier of chlorophyll content. Chlorophyll reduction increased the time to reproductive maturity, decreased stalk width, and had complex effects on plant height. We explored these effects by genome wide association studies of F1 hybrids crosses of Oy1-N1989 to a maize diversity panel. The delay in reproductive maturity in Oy1-N1989/+ mutants neither altered the vegetative-to-adult phase change nor total leaf number but did slow the rate of organ emergence from the whorl. In addition to the allele at oy1, analysis of tetrapyrrole, heme, and chlorophyll biosynthesis pathway genes identified multiple modifiers of mutant traits. Consistent with organ emergence, rather than a developmental delay, affecting the reproductive delay of Oy1-N1989/+ mutants, known flowering time regulators did not affect variation in the reproductive delay affected by chlorophyll loss. Plant height showed a complex relationship with chlorophyll. The Oy1-N1989/+ mutant F1 plants with a modest reduction in chlorophyll were taller than their wildtype siblings, but F1 plants with dramatically decreased chlorophyll were shorter than their wildtype siblings. Perturbations in the biosynthesis of the phytochrome chromophore increased plant height in wild-type plants but dramatically reduced plant height and chlorophyll contents of Oy1-N1989/+ mutants. The mechanism by which the interaction between chlorophyll and bilin affects height and chlorophyll remains to be explored.