Genome wide association mapping of epi-cuticular wax genes in Sorghum bicolor

Elango, Dinakaran; Xue, Weiya; Chopra, Surinder

doi:10.1007/s12298-020-00848-5

Cited by 18 publications

(11 citation statements)

References 71 publications

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“…We believe that this is the first report evaluating waxes in pea by GWAS. Greater lamina and petiole wax concentrations were associated with the stress environment, which is in agreement with several studies conducted on different crops species [ 14 , 16 , 45 , 46 ]. Abiotic stresses triggered increased wax concentrations as a stress-resistance response [ 12 , 47 ].…”

Section: Discussionsupporting

confidence: 92%

“…ABC transporters are necessary for wax export from the site of synthesis to the outer membrane in plants [ 50 ]. Based on a GWAS study conducted on sorghum, Elango et al [ 16 ] reported several putative genes responsible for epicuticular-wax biosynthesis and export from the synthesis site to the outer plant membrane. Selecting genotypes for high epicuticular wax is essential in breeding stress-resistant crop cultivars for increased adaptation to environmental stresses.…”

Section: Discussionmentioning

confidence: 99%

“…As a drought-tolerance trait, leaf wax minimizes the excess loss of water through stomatal and non-stomatal transpiration [ 12 ]. Surface wax has been extensively studied as a stress resistance trait in multiple crops including pea, sorghum and wheat [ 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Association Mapping for Heat and Drought Adaptive Traits in Pea

Tafesse

Gali

Lachagari

et al. 2021

Genes

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Heat and drought, individually or in combination, limit pea productivity. Fortunately, substantial genetic diversity exists in pea germplasm for traits related to abiotic stress resistance. Understanding the genetic basis of resistance could accelerate the development of stress-adaptive cultivars. We conducted a genome-wide association study (GWAS) in pea on six stress-adaptive traits with the aim to detect the genetic regions controlling these traits. One hundred and thirty-five genetically diverse pea accessions were phenotyped in field studies across three or five environments under stress and control conditions. To determine marker trait associations (MTAs), a total of 16,877 valuable single nucleotide polymorphisms (SNPs) were used in association analysis. Association mapping detected 15 MTAs that were significantly (p ≤ 0.0005) associated with the six stress-adaptive traits averaged across all environments and consistent in multiple individual environments. The identified MTAs were four for lamina wax, three for petiole wax, three for stem thickness, two for the flowering duration, one for the normalized difference vegetation index (NDVI), and two for the normalized pigment and chlorophyll index (NPCI). Sixteen candidate genes were identified within a 15 kb distance from either side of the markers. The detected MTAs and candidate genes have prospective use towards selecting stress-hardy pea cultivars in marker-assisted selection.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Association Mapping for Heat and Drought Adaptive Traits in Pea

Tafesse

Gali

Lachagari

et al. 2021

Genes

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“…In addition, GLY-ASP-SER-LEU ESTERASE/LIPASE (GDSL lipase) is required for cutin polymerization in the developing cuticle ( Girard et al, 2012 ; Yeats and Rose, 2013 ), and the degree of pectin esterification is critical to cuticle structure by facilitating the diffusion of cutin precursors ( Bakan and Marion, 2017 ; Philippe et al, 2020 ). Transcription factors have also been identified to regulate various steps in cuticle development ( Borisjuk et al, 2014 ; Elango et al, 2020 ), but how these transcription factors, biosynthesis, and transport processes work together is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Integrating GWAS and TWAS to elucidate the genetic architecture of maize leaf cuticular conductance

et al. 2022

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The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed. Dissecting the genetic architecture of natural variation for maize (Zea mays L.) leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we performed an integrated genome- and transcriptome-wide association study (GWAS/TWAS) to identify candidate genes putatively regulating variation in leaf gc. Of the 22 plausible candidate genes identified, four were predicted to be involved in cuticle precursor biosynthesis and export, two in cell wall modification, nine in intracellular membrane trafficking, and seven in the regulation of cuticle development. A gene encoding an INCREASED SALT TOLERANCE1-LIKE1 (ISTL1) protein putatively involved in intracellular protein and membrane trafficking was identified in GWAS and TWAS as the strongest candidate causal gene. A set of maize nested near-isogenic lines that harbor the ISTL1 genomic region from eight donor parents were evaluated for gc, confirming the association between gc and ISTL1 in a haplotype-based association analysis. The findings of this study provide insights into the role of regulatory variation in the development of the maize leaf cuticle and will ultimately assist breeders to develop drought-tolerant maize for target environments.

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“… Mizuno et al (2013) reported that an ABC transporter gene, Sb06g023280 , is responsible for epi-cuticular wax biosynthesis in Sorghum. Elango et al (2020) assessed the epicuticular wax variability in the extensive genetic pool of Sorghum, and a genome-wide association mapping study showed genic regions associated with epicuticular wax production. Hence, the enhanced epicuticular wax content and the deposition of wax crystals on the leaf surfaces are essential components of plants for enhanced drought tolerance to overcome non-stomatal water loss.…”

Section: Discussionmentioning

confidence: 99%

De novo Transcriptome Analysis of Drought-Adapted Cluster Bean (Cultivar RGC-1025) Reveals the Wax Regulatory Genes Involved in Drought Resistance

Reddy

Johnson²,

Kumar

et al. 2022

Front. Plant Sci.

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Cluster bean (Cyamopsis tetragonoloba L.) is one of the multipurpose underexplored crops grown as green vegetable and for gum production in dryland areas. Cluster bean is known as relatively tolerant to drought and salinity stress. To elucidate the molecular mechanisms involved in the drought tolerance of cluster bean cultivar RGC-1025, RNA sequencing (RNA-seq) of the drought-stressed and control samples was performed. De novo assembly of the reads resulted in 66,838 transcripts involving 203 pathways. Among these transcripts, differentially expressed gene (DEG) analysis resulted in some of the drought-responsive genes expressing alpha dioxygenase 2, low temperature-induced 65 kDa protein (LDI65), putative vacuolar amino acid transporter, and late embryogenesis abundant protein (LEA 3). The analysis also reported drought-responsive transcription factors (TFs), such as NAC, WRKY, GRAS, and MYB families. The relative expression of genes by qRT-PCR revealed consistency with the DEG analysis. Key genes involved in the wax biosynthesis pathway were mapped using the DEG data analysis. These results were positively correlated with epicuticular wax content and the wax depositions on the leaf surfaces, as evidenced by scanning electron microscope (SEM) image analysis. Further, these findings support the fact that enhanced wax deposits on the leaf surface had played a crucial role in combating the drought stress in cluster beans under drought stress conditions. In addition, this study provided a set of unknown genes and TFs that could be a source of engineering tolerance against drought stress in cluster beans.

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Genome wide association mapping of epi-cuticular wax genes in Sorghum bicolor

Cited by 18 publications

References 71 publications

Genome-Wide Association Mapping for Heat and Drought Adaptive Traits in Pea

Genome-Wide Association Mapping for Heat and Drought Adaptive Traits in Pea

Integrating GWAS and TWAS to elucidate the genetic architecture of maize leaf cuticular conductance

De novo Transcriptome Analysis of Drought-Adapted Cluster Bean (Cultivar RGC-1025) Reveals the Wax Regulatory Genes Involved in Drought Resistance

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