Genome-wide association mapping of lentil (Lens culinaris Medikus) prebiotic carbohydrates toward improved human health and crop stress tolerance

Johnson, Nathan; Boatwright, J. Lucas; Bridges, William C.; Thavarajah, Pushparajah; Kumar, Shiv; Shipe, E. R.; Thavarajah, Dil

doi:10.1038/s41598-021-93475-3

Cited by 24 publications

(18 citation statements)

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“…Total RFO concentration of lentil cultivars varied from 4.5 to 5.5 moles 100 g –1 flour. Johnson et al (2021) found 7 to 31% variation for RFOs in lentil seeds from 143 accessions. Significant differences were observed between different years of lentil harvest.…”

Section: Raffinose Family Oligosaccharides Diversity In Cropsmentioning

confidence: 95%

Raffinose Family Oligosaccharides: Friend or Foe for Human and Plant Health?

et al. 2022

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Raffinose family oligosaccharides (RFOs) are widespread across the plant kingdom, and their concentrations are related to the environment, genotype, and harvest time. RFOs are known to carry out many functions in plants and humans. In this paper, we provide a comprehensive review of RFOs, including their beneficial and anti-nutritional properties. RFOs are considered anti-nutritional factors since they cause flatulence in humans and animals. Flatulence is the single most important factor that deters consumption and utilization of legumes in human and animal diets. In plants, RFOs have been reported to impart tolerance to heat, drought, cold, salinity, and disease resistance besides regulating seed germination, vigor, and longevity. In humans, RFOs have beneficial effects in the large intestine and have shown prebiotic potential by promoting the growth of beneficial bacteria reducing pathogens and putrefactive bacteria present in the colon. In addition to their prebiotic potential, RFOs have many other biological functions in humans and animals, such as anti-allergic, anti-obesity, anti-diabetic, prevention of non-alcoholic fatty liver disease, and cryoprotection. The wide-ranging applications of RFOs make them useful in food, feed, cosmetics, health, pharmaceuticals, and plant stress tolerance; therefore, we review the composition and diversity of RFOs, describe the metabolism and genetics of RFOs, evaluate their role in plant and human health, with a primary focus in grain legumes.

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Section: Raffinose Family Oligosaccharides Diversity In Cropsmentioning

confidence: 95%

Raffinose Family Oligosaccharides: Friend or Foe for Human and Plant Health?

et al. 2022

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“…especially within allergen-and gluten-free markets [7,37,38]. Our results indicate organic dry peas are rich in prebiotic carbohydrates (12.5-19.8 g/100 g), providing 63-99% of the RDA for adults (Table 5).…”

Section: Plos Onementioning

confidence: 67%

“…Due to the dietary nature of human metabolic disorders related to obesity, solutions will necessarily focus on a diet-i.e., a cup of pulses a day provides 13-15 g of prebiotic carbohydrates and a range of micronutrients [6,9,44]. Changing the levels of these prebiotic carbohydrates is possible by developing molecular markers for marker-assisted breeding with conventional breeding methods in pulse crops; however, genome-wide association mapping studies with diverse populations at several field locations are essential to avoid the yield and protein penalty by changing certain carbohydrates as a result of the quantitative nature of these nutritional traits [37,45]. Pulses crops, including dry pea, also known as "poor man's meat," are low in fat and provide significant quantities of dietary protein (20-25 g/100 g) and minerals [7,46].…”

Section: Plos Onementioning

confidence: 99%

“…Similar to previous studies, our results show organic dry peas are also rich in Fe, Zn, and Se but not a good Ca source (Table 5). Integrating genome-wide research approaches with conventional plant breeding to identify genetic markers associated with these mineral traits could significantly accelerate biofortification efforts by enabling molecular screening of exotic germplasm collections and elite cultivars [23,37]. No research has been conducted regarding reducing the yield gap without compromising nutritional yield and developing genomic tools for marker-assisted breeding of organic pulse cultivars, i.e., biofortification of organic pulse grains.…”

Section: Plos Onementioning

confidence: 99%

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Organic dry pea (Pisum sativum L.) biofortification for better human health

et al. 2022

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A primary criticism of organic agriculture is its lower yield and nutritional quality compared to conventional systems. Nutritionally, dry pea (Pisum sativum L.) is a rich source of low digestible carbohydrates, protein, and micronutrients. This study aimed to evaluate dry pea cultivars and advanced breeding lines using on-farm field selections to inform the development of biofortified organic cultivars with increased yield and nutritional quality. A total of 44 dry pea entries were grown in two USDA-certified organic on-farm locations in South Carolina (SC), United States of America (USA) for two years. Seed yield and protein for dry pea ranged from 61 to 3833 kg ha-1 and 12.6 to 34.2 g/100 g, respectively, with low heritability estimates. Total prebiotic carbohydrate concentration ranged from 14.7 to 26.6 g/100 g. A 100-g serving of organic dry pea provides 73.5 to 133% of the recommended daily allowance (%RDA) of prebiotic carbohydrates. Heritability estimates for individual prebiotic carbohydrates ranged from 0.27 to 0.82. Organic dry peas are rich in minerals [iron (Fe): 1.9–26.2 mg/100 g; zinc (Zn): 1.1–7.5 mg/100 g] and have low to moderate concentrations of phytic acid (PA:18.8–516 mg/100 g). The significant cultivar, location, and year effects were evident for grain yield, thousand seed weight (1000-seed weight), and protein, but results for other nutritional traits varied with genotype, environment, and interactions. “AAC Carver,” “Jetset,” and “Mystique” were the best-adapted cultivars with high yield, and “CDC Striker,” “Fiddle,” and “Hampton” had the highest protein concentration. These cultivars are the best performing cultivars that should be incorporated into organic dry pea breeding programs to develop cultivars suitable for organic production. In conclusion, organic dry pea has potential as a winter cash crop in southern climates. Still, it will require selecting diverse genetic material and location sourcing to develop improved cultivars with a higher yield, disease resistance, and nutritional quality.

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“…], barley ( Hordeum vulgare L.), cotton ( Gossypium hirsutum L.), and the model plant species Arabidopsis ( Tibbs Cortes et al, 2021 ). However, GWAS application in lentil is very limited with studies mainly restricted to traits such as carbohydrate, iron, zinc content, anthracnose, ascochyta, aphanomyces resistance, and agronomic traits ( Table 2 ; Singh et al, 2017a ; Khazaei et al, 2018 ; Kumar et al, 2019 ; Ma et al, 2020 ; Dadu et al, 2021 ; Gela et al, 2021a ; Johnson et al, 2021 ). GWAS studies are urgently needed in lentils to understand complex traits related to abiotic and biotic stress, plant development, and nutritional aspects.…”

Section: Genomic Innovations Reveal a New Genetic Landscape For Lenti...mentioning

confidence: 99%

Omics Path to Increasing Productivity in Less-Studied Crops Under Changing Climate—Lentil a Case Study

et al. 2022

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Conventional breeding techniques for crop improvement have reached their full potential, and hence, alternative routes are required to ensure a sustained genetic gain in lentils. Although high-throughput omics technologies have been effectively employed in major crops, less-studied crops such as lentils have primarily relied on conventional breeding. Application of genomics and transcriptomics in lentils has resulted in linkage maps and identification of QTLs and candidate genes related to agronomically relevant traits and biotic and abiotic stress tolerance. Next-generation sequencing (NGS) complemented with high-throughput phenotyping (HTP) technologies is shown to provide new opportunities to identify genomic regions and marker-trait associations to increase lentil breeding efficiency. Recent introduction of image-based phenotyping has facilitated to discern lentil responses undergoing biotic and abiotic stresses. In lentil, proteomics has been performed using conventional methods such as 2-D gel electrophoresis, leading to the identification of seed-specific proteome. Metabolomic studies have led to identifying key metabolites that help differentiate genotypic responses to drought and salinity stresses. Independent analysis of differentially expressed genes from publicly available transcriptomic studies in lentils identified 329 common transcripts between heat and biotic stresses. Similarly, 19 metabolites were common across legumes, while 31 were common in genotypes exposed to drought and salinity stress. These common but differentially expressed genes/proteins/metabolites provide the starting point for developing high-yielding multi-stress-tolerant lentils. Finally, the review summarizes the current findings from omic studies in lentils and provides directions for integrating these findings into a systems approach to increase lentil productivity and enhance resilience to biotic and abiotic stresses under changing climate.

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Genome-wide association mapping of lentil (Lens culinaris Medikus) prebiotic carbohydrates toward improved human health and crop stress tolerance

Cited by 24 publications

References 50 publications

Raffinose Family Oligosaccharides: Friend or Foe for Human and Plant Health?

Raffinose Family Oligosaccharides: Friend or Foe for Human and Plant Health?

Organic dry pea (Pisum sativum L.) biofortification for better human health

Omics Path to Increasing Productivity in Less-Studied Crops Under Changing Climate—Lentil a Case Study

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