Genome-wide association study and transcriptome analysis reveal key genes controlling fruit branch angle in cotton

Shao, Panxia; Peng, Yabin; Wu, Yuanlong; Wang, Jing; Pan, Zhenyuan; Yang, Yang; Aini, Nurimanguli; Chunping, Guo; Shui, Guangling; Lei, Chao; Tian, Xin; An, Qiushuang; Yang, Qingyong; You, Chunyuan; Lu, Lu; Zhang, Xianlong; Wang, Maojun; Nie, Xiaoqin

doi:10.3389/fpls.2022.988647

Cited by 5 publications

(2 citation statements)

References 57 publications

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“…Put the samples rinsed in xylene three times for 5 min each time, and the tissue sections were mounted. Finally, a Panoramic MIDI automatic digital slide scanner (3DHISTECH Ltd., Budapest, Hungary) was used for observation images (Shao et al 2022).…”

Section: Para N Section Observation Of Cotton Anthersmentioning

confidence: 99%

Identification and cytological observation of CMS in cotton

PEI,

LIU,

GUO

et al. 2023

Preprint

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Background The utilization of male sterility is essential for producing hybrid seeds, and comprehending the mechanism of male sterility is the foundation for developing sterile germplasm resources. However, only a few cytoplasmic male sterility (CMS) lines of cotton have been produced due to various hindrances. Inadequate agronomic traits and incomplete sterility, weak resilience of restorer lines and the difficulty in combining strong dominance all serve as primary impediments to the advancement of CMS in cotton. Therefore, identifying and cytologically observing of CMS in cotton will provide new insights for heterosis utilization. Results Two F2 segregating populations of cotton were constructed from cytoplasmic sterile lines (HaA and 01A, maternal) and restorer lines (HaR and 26R, paternal). Genetic analysis of F2 segregation populations showed that the separation ratio of fertile to sterile plants was 3:1. Phenotypic analysis indicated that there was no significant difference in the development process of flower buds between sterile and fertile plants. Compared to fertile plants, sterile plants exhibit smaller floral organs, shortened filament length and anther atrophy on the day of flowering. Scanning electron microscopy (SEM) of two F2 populations revealed morphological variations in the folds of the anther epidermis. Analysis of cell level differences showed that there was no significant difference in pollen development before the pollen maturation stage. However, between pollen maturation stage and flowering stage, the tapetum layer of sterile plants degenerated prematurely, the pollen grains were abnormal, pollen cytoplasmic degradation and gradually hollowed out. Conclusion The sterile trait is controlled by a single recessive gene. The floral morphology of the sterile plant was characterized by stamen atrophy and abnormal anthers. Pollen abortion occurs from the time of pollen maturity until flowering. Degradation of pollen grains and the formation of empty pollen grains due to the premature degradation of the tapetum are presumed to be the main cause for pollen abortion.

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Section: Para N Section Observation Of Cotton Anthersmentioning

confidence: 99%

Identification and cytological observation of CMS in cotton

PEI,

LIU,

GUO

et al. 2023

Preprint

Self Cite

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“…Sympodial branches typically grow almost laterally from the main stem and are largely responsible for bearing the reproductive buds (squares). Fruiting branch angles ranging from 36.86° to 64.56° have been reported ( Shao et al., 2022 ). With multiple branch types, cotton plants can allocate resources efficiently to both vegetative growth and reproduction ( Gore, 1935 ; McGarry et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic and functional analysis of tiller angle control homeologs in allotetraploid cotton

Kangben,

Kumar,

et al. 2024

Front. Plant Sci.

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IntroductionPlants can adapt their growth to optimize light capture in competitive environments, with branch angle being a crucial factor influencing plant phenotype and physiology. Decreased branch angles in cereal crops have been shown to enhance productivity in high-density plantings. The Tiller Angle Control (TAC1) gene, known for regulating tiller inclination in rice and corn, has been found to control branch angle in eudicots. Manipulating TAC1 in field crops like cotton offers the potential for improving crop productivity.MethodsUsing a homolog-based methodology, we examined the distribution of TAC1-related genes in cotton compared to other angiosperms. Furthermore, tissue-specific qPCR analysis unveiled distinct expression patterns of TAC1 genes in various cotton tissues. To silence highly expressed specific TAC1 homeologs in the stem, we applied CRISPR-Cas9 gene editing and Agrobacterium-mediated transformation, followed by genotyping and subsequent phenotypic validation of the mutants.ResultsGene duplication events of TAC1 specific to the Gossypium lineage were identified, with 3 copies in diploid progenitors and 6 copies in allotetraploid cottons. Sequence analysis of the TAC1 homeologs in Gossypium hirsutum revealed divergence from other angiosperms with 1-2 copies, suggesting possible neo- or sub-functionalization for the duplicated copies. These TAC1 homeologs exhibited distinct gene expression patterns in various tissues over developmental time, with elevated expression of A11G109300 and D11G112200, specifically in flowers and stems, respectively. CRISPR-mediated loss of these TAC1 homeologous genes resulted in a reduction in branch angle and altered petiole angles, and a 5 to 10-fold reduction in TAC1 expression in the mutants, confirming their role in controlling branch and petiole angles. This research provides a promising strategy for genetically engineering branch and petiole angles in commercial cotton varieties, potentially leading to increased productivity.

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Genome-wide association study identifies GhSAL1 affects cold tolerance at the seedling emergence stage in upland cotton (Gossypium hirsutum L.)

Shen

Zhang

et al. 2023

Theor Appl Genet

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Genome-wide association study and transcriptome analysis reveal key genes controlling fruit branch angle in cotton

Cited by 5 publications

References 57 publications

Identification and cytological observation of CMS in cotton

Identification and cytological observation of CMS in cotton

Phylogenetic and functional analysis of tiller angle control homeologs in allotetraploid cotton

Genome-wide association study identifies GhSAL1 affects cold tolerance at the seedling emergence stage in upland cotton (Gossypium hirsutum L.)

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