Genome-Wide Characterization and Expression Analysis of NHX Gene Family under Salinity Stress in Gossypium barbadense and Its Comparison with Gossypium hirsutum

Akram, Umar; Song, Yuhan; Liang, Chengzhen; Abid, Muhammad Ali; Askari, Muhammad; Myat, Aye Aye; Abbas, Mubashir; Malik, Waqas; Ali, Zulfiqar; Guo, Sandui; Zhang, Rui; Meng, Zhigang

doi:10.3390/genes11070803

Cited by 34 publications

(26 citation statements)

References 123 publications

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“…Moreover, NHX1 regulated Cd 2+ and H + flow during short-term Cd 2+ shock and confirmed that it could enhance tolerance during Cd 2+ stress [44]. Recent studies have shown that NHX2 homologues had a high expression under salinity stress at higher time intervals in G. barbadense and G. hirsutum [45]. The expressions of NHX were up-regulated in root tissues of wheat under salinity stress [46].…”

Section: Roles Of Rscpa Genes In Response To Different Abiotic Stressesmentioning

confidence: 80%

Genome-Wide Identification and Functional Characterization of the Cation Proton Antiporter (CPA) Family Related to Salt Stress Response in Radish (Raphanus sativus L.)

Wang

Ying

Yang

et al. 2020

IJMS

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The CPA (cation proton antiporter) family plays an essential role during plant stress tolerance by regulating ionic and pH homeostasis of the cell. Radish fleshy roots are susceptible to abiotic stress during growth and development, especially salt stress. To date, CPA family genes have not yet been identified in radish and the biological functions remain unclear. In this study, 60 CPA candidate genes in radish were identified on the whole genome level, which were divided into three subfamilies including the Na+/H+ exchanger (NHX), K+ efflux antiporter (KEA), and cation/H+ exchanger (CHX) families. In total, 58 of the 60 RsCPA genes were localized to the nine chromosomes. RNA-seq. data showed that 60 RsCPA genes had various expression levels in the leaves, roots, cortex, cambium, and xylem at different development stages, as well as under different abiotic stresses. RT–qPCR analysis indicated that all nine RsNHXs genes showed up regulated trends after 250 mM NaCl exposure at 3, 6, 12, and 24h. The RsCPA31 (RsNHX1) gene, which might be the most important members of the RsNHX subfamily, exhibited obvious increased expression levels during 24h salt stress treatment. Heterologous over-and inhibited-expression of RsNHX1 in Arabidopsis showed that RsNHX1 had a positive function in salt tolerance. Furthermore, a turnip yellow mosaic virus (TYMV)-induced gene silence (VIGS) system was firstly used to functionally characterize the candidate gene in radish, which showed that plant with the silence of endogenous RsNHX1 was more susceptible to the salt stress. According to our results we provide insights into the complexity of the RsCPA gene family and a valuable resource to explore the potential functions of RsCPA genes in radish.

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Section: Roles Of Rscpa Genes In Response To Different Abiotic Stressesmentioning

confidence: 80%

Genome-Wide Identification and Functional Characterization of the Cation Proton Antiporter (CPA) Family Related to Salt Stress Response in Radish (Raphanus sativus L.)

Wang

Ying

Yang

et al. 2020

IJMS

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“…The function of plant vacuole NHX antiporter has been identified and expressed in an exogenous system to enhance the salt tolerance of plants. Akram et al (2020) found that the NHX gene was up-regulated under salt stress, and Yokoi et al (2002) found that AtNHX1 had higher transcript abundance during salt stress ( Yarra, 2019 ). The results indicated that the expression of NHX genes responded to salt stress during plant growth, which played a very important role in plant growth.…”

Section: Discussionmentioning

confidence: 99%

“…A change in membrane permeability destroys the normal operation of transporters, causing plants to absorb additional sodium ions from the environment, affecting the absorption of other ions and causing nutritional imbalance. Na + /H + antiporters play a key role in plant development and tolerance to salt stress ( Akram et al, 2020 ). In response to the external influence on plants, the ions and water in plants are balanced through their own ion channels.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Expression Analysis of the Na+/H+ Exchanger Gene Family in Capsicum annuum L.

Luo

Yang

Luo³

et al. 2021

Front. Genet.

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The Na+/H+ exchangers (NHXs) are a class of transporters involved in ion balance during plant growth and abiotic stress. We performed systematic bioinformatic identification and expression-characteristic analysis of CaNHX genes in pepper to provide a theoretical basis for pepper breeding and practical production. At the whole-genome level, the members of the CaNHX gene family of cultivated and wild pepper were systematically identified using bioinformatics methods. Sequence alignment and phylogenetic tree construction were performed using MEGA X software, and the gene functional domain, conserved motif, and gene structure were analyzed and visualized. At the same time, the co-expression network of CaNHX genes was analyzed, and salt-stress analysis and fluorescence quantitative verification of the Zunla-1 cultivar under stress conditions were performed. A total of 9 CaNHX genes were identified, which have typical functional domains of the Na+/H+ exchanger gene. The physical and chemical properties of the protein showed that the protein was hydrophilic, with a size of 503–1146 amino acids. Analysis of the gene structure showed that Chr08 was the most localized chromosome, with 8–24 exons. Cis-acting element analysis showed that it mainly contains cis-acting elements such as light response, salicylic acid response, defense, and stress response. Transcriptom and co-expression network analysis showed that under stress, the co-expressed genes of CaNHX genes in roots and leaves were more obvious than those in the control group, including ABA, IAA, and salt. The transcriptome and co-expression were verified by qRT-PCR. In this study, the CaNHX genes were identified at the genome level of pepper, which provides a theoretical foundation for improving the stress resistance, production, development, and utilization of pepper in genetic breeding.

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“…Furthermore, transgenic cotton plants expressing AtNHX1 reportedly produce larger biomass and more fibers when treated with 200 mM NaCl in a greenhouse compared with control plants, implying that AtNHX1 can increase cotton salt stress tolerance [ 40 ]. Researchers found that NHX members in cotton could be involved in various developmental processes and stress responses by maintaining the turgor pressure, pH, and ionic homeostasis [ 41 ]. However, the functions of cotton endosomal-type NHX members related to Na + /H + exchange and localization in plant cells remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the Gossypium hirsutum NHX Genes Reveals That the Endosomal-Type GhNHX4A Is Critical for the Salt Tolerance of Cotton

Ren

Zhou

et al. 2020

IJMS

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Soil salinization, which is primarily due to excessive Na+ levels, is a major abiotic stress adversely affecting plant growth and development. The Na+/H+ antiporter (NHX) is a transmembrane protein mediating the transport of Na+ or K+ and H+ across the membrane to modulate the ionic balance of plants in response to salt stress. Research regarding NHXs has mainly focused on the vacuolar-type NHX family members. However, the biological functions of the endosomal-type NHXs remain relatively uncharacterized. In this study, 22 NHX family members were identified in Gossypium hirsutum. A phylogenetic analysis divided the GhNHX genes into two categories, with 18 and 4 in the vacuolar and endosomal groups, respectively. The chromosomal distribution of the NHX genes revealed the significant impact of genome-wide duplication during the polyploidization process on the number of GhNHX genes. Analyses of gene structures and conserved motifs indicated that GhNHX genes in the same phylogenetic cluster are conserved. Additionally, the salt-induced expression patterns confirmed that the expression levels of most of the GhNHX genes are affected by salinity. Specifically, in the endosomal group, GhNHX4A expression was substantially up-regulated by salt stress. A yeast functional complementation test proved that GhNHX4A can partially restore the salt tolerance of the salt-sensitive yeast mutant AXT3. Silencing GhNHX4A expression decreased the resistance of cotton to salt stress because of an increase in the accumulation of Na+ in stems and a decrease in the accumulation of K+ in roots. The results of this study may provide the basis for an in-depth characterization of the regulatory functions of NHX genes related to cotton salt tolerance, especially the endosomal-type GhNHX4A. Furthermore, the presented data may be useful for selecting appropriate candidate genes for the breeding of new salt-tolerant cotton varieties.

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Genome-Wide Characterization and Expression Analysis of NHX Gene Family under Salinity Stress in Gossypium barbadense and Its Comparison with Gossypium hirsutum

Cited by 34 publications

References 123 publications

Genome-Wide Identification and Functional Characterization of the Cation Proton Antiporter (CPA) Family Related to Salt Stress Response in Radish (Raphanus sativus L.)

Genome-Wide Identification and Functional Characterization of the Cation Proton Antiporter (CPA) Family Related to Salt Stress Response in Radish (Raphanus sativus L.)

Molecular Characterization and Expression Analysis of the Na+/H+ Exchanger Gene Family in Capsicum annuum L.

Genome-Wide Identification of the Gossypium hirsutum NHX Genes Reveals That the Endosomal-Type GhNHX4A Is Critical for the Salt Tolerance of Cotton

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