Genome‐wide characterization of caspase genes in Japanese pufferfish, <i>Takifugu rubripes</i>, and expression profiles in response to <i>Vibrio harveyi</i> infection

Yang, Xu; Liu, Xia; Yang, Zhen; Guan, Yude; Zhao, Ruihu; Peng, Hongyu; Cao, Xinyu; Gao, Minghong; Wang, Shuhui; Jiang, Chen

doi:10.1111/jwas.12904

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…In Japanese pufferfish, 10 caspase genes were identified through bioinformatics analyses and detected in all examined tissues. The expression of 10 caspases significantly varied in a time‐dependent manner after Vibrio harveyi infection, suggesting their roles in the antibacterial process of Japanese pufferfish (Yang et al, 2022).…”

Section: Disease Resistancementioning

confidence: 99%

Presentations by young Chinese aquaculture scientists: From the 2021 China Society of Fisheries—Annual Conference of Youth Scholars

Wang

2022

J World Aquaculture Soc

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Young scientists are the most active force driving scientific advancement and technological innovation. Nonetheless, young scholars also face challenges such as heavy workloads, shortage of research-focused hours, high competition in grant application, and securing an academic job, among others. Mentorship from supervisors and senior peers is important to encourage and reassure the scientific endeavors of young scholars. Favorable policies and activities arranged by public agencies or academic societies are also essential for nurturing and promoting the successful establishment of young scholars. Chinese young scientists have been making great contributions to aquaculture research and applications. The China Society of Fisheries organized the Annual Conference of Youth Scholars in Shanghai on October 18, 2021. There were over 300 attendees to this meeting, which covered the topics of aquaculture techniques and equipment, biotechnologies and breeding, nutrition and feeds, diseases and quality security, aquaculture resources and conservation, and aquaculture economy and management. Here, we summarize some representative work by the attendees who were selected for this special issue, which partially reflects the recent research focus on young aquaculture scientists in China.The healthy and sustainable development of aquaculture depends heavily on research on nutritional feeds in terms of precise nutritional requirements of aquatic animals. The largemouth bass, Micropterus salmoides, characterized by its fast growth and delicious taste, is currently a major species of freshwater aquaculture in China. According to the China Fishery Statistical Yearbook report, the total production of farmed largemouth bass in 2020 was more than 619,500 tons, and the national demand for this fish is also increasing yearly (China Fishery Yearbook, 2021). To date, the requirement of each vitamin of the largemouth bass has its own specific and irreplaceable function, so it is necessary to investigate the optimal level of usage one by one. The requirement for fat-soluble vitamins, including vitamins

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Section: Disease Resistancementioning

confidence: 99%

Presentations by young Chinese aquaculture scientists: From the 2021 China Society of Fisheries—Annual Conference of Youth Scholars

Wang

2022

J World Aquaculture Soc

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Characterization of caspase gene family in Sebastes schlegelii and their expression profiles under Aeromonas salmonicida and Vibrio anguillarum infection

Yu,

Yan,

Wang

et al. 2024

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Molecular Characterization and Antibacterial Potential of Goose-Type Lysozyme from Japanese Pufferfish (Takifugu rubripes)

Cao,

Yang,

Gao

et al. 2023

Fishes

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Lysozyme plays a crucial role in the innate immune response against bacterial phagocytosis by hydrolyzing the peptidoglycan layer of the bacterial cell wall. In this study, we characterized a goose-type lysozyme gene (TrLysG) in Japanese pufferfish. It is made up of an ORF of 573 bp that encodes a polypeptide of 190 amino acids. TrLysG includes a characteristic bacterial soluble lytic transglycosylase (SLT) domain, which contains three catalytic residues (Glu71, Asp84 and Asp95) and a highly conserved GLMQ motif (Gly90, Leu91, Met92 and Gln93). Phylogenetic analyses revealed that TrLysG is clustered together with its counterparts from other teleost fishes. Furthermore, mRNA expression analyses showed that TrLysG was highly expressed in healthy mucosal tissues (intestines and gills), and considerably up-regulated in response to Vibrio harveyi infection in the intestines, gills, and liver. At pH 6 and 55 °C, the pure recombinant TrLysG (rTrLysG) exhibits optimum activity. It also displayed antimicrobial activity against three Gram-positive bacteria (Streptococcus parauberis, Staphylococcus pasteuri and Staphylococcus epidermidis) as well as five Gram-negative bacteria (Shewanella, Aeromonas hydrophila, Escherichia coli, Vibrio parahaemolyticus and V. harveyi). Our results highlighted the significant role of TrLysG in immune defense against invading pathogens, thereby contributing to the prevention and alleviation of disease spread in aquaculture.

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Genome‐wide characterization of caspase genes in Japanese pufferfish, Takifugu rubripes, and expression profiles in response to Vibrio harveyi infection

Cited by 3 publications

References 41 publications

Presentations by young Chinese aquaculture scientists: From the 2021 China Society of Fisheries—Annual Conference of Youth Scholars

Presentations by young Chinese aquaculture scientists: From the 2021 China Society of Fisheries—Annual Conference of Youth Scholars

Characterization of caspase gene family in Sebastes schlegelii and their expression profiles under Aeromonas salmonicida and Vibrio anguillarum infection

Molecular Characterization and Antibacterial Potential of Goose-Type Lysozyme from Japanese Pufferfish (Takifugu rubripes)

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