Genome-wide characterization of chalcone synthase genes in sweet cherry and functional characterization of CpCHS1 under drought stress

Hou, Qiandong; Li, Shuang; Shang, Chunqiong; Zhuang, Wen; Cai, Xiaowei; Hong, Yi; Qiao, Guo

doi:10.3389/fpls.2022.989959

Cited by 23 publications

(8 citation statements)

References 75 publications

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“…PtHMGR is "anchored" to specific parts of the cytoplasmic matrix and exerts a catalytic function between its transmembrane domain and membrane lipid [ 43 ]. PtCHS has no transmembrane region, is synthesized in the cytoplasm, and interacts directly with metabolic substrates in the cytoplasmic matrix to exercise their catalytic function [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd

Liu,

Ma,

Mao

et al. 2024

PLoS ONE

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Triterpenoid saponins and flavonoids have several pharmacological activities against P. tenuifolia. The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and chalcone synthase (CHS) are the rate-limiting enzymes of triterpenoid saponin and flavonoid biosynthesis, respectively. In this study, HMGR and CHS genes were cloned from P. tenuifolia, and their bioinformatics analyses and tissue-specific expression were investigated. The results showed that the HMGR and CHS genes were successfully cloned, separately named the PtHMGR gene (NCBI accession: MK424118) and PtCHS gene (NCBI accession: MK424117). The PtHMGR gene is 2323 bp long, has an open reading frame (ORF) of 1782 bp, and encods 593 amino acids. The PtCHS gene is 1633 bp long with an ORF of 1170 bp, encoding 389 amino acids. PtHMGR and PtCHS were both hydrophobic, not signal peptides or secreted proteins, containing 10 conserved motifs. PtHMGR and PtCHS separately showed high homology with HMGR and CHS proteins from other species, and their secondary structures mainly included α-helix and random curl. The tertiary structure of PtHMGR was highly similarity to that the template 7ULI in RCSB PDB with 92.0% coverage rate. The HMG-CoA-binding domain of PtHMGR is located at 173–572 amino acid residues, including five bound sites. The tertiary structure of PtCHS showed high consistency with the template 1I86 in RCSB PDB with 100% coverage rate, contained malonyl CoA and 4-coumaroyl-CoA linkers. The expression of PtHMGR and PtCHS is tissue-specific. PtHMGR transcripts were mainly accumulated in roots, followed by leaves, and least in stems, and were significantly positively correlated with the contents of total saponin and tenuifolin. PtCHS was highly expressed in the stems, followed by the leaves, with low expression in the roots. PtCHS transcripts showed a significant positive correlation with total flavonoids content, however, they were significantly negatively correlated with the content of polygalaxanthone III (a type of flavonoids). This study provided insight for further revealing the roles of PtHMGR and PtCHS.

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Section: Resultsmentioning

confidence: 99%

HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd

Liu,

Ma,

Mao

et al. 2024

PLoS ONE

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“…JA could regulate the biosynthesis of tanshinones via the JAZ9-MYB76 complex in S. miltiorrhiza [ 75 ]. And GA could promote hairy roots growth and increase the contents of tanshinones [ 76 ]. Therefore, we speculated that these genes have dual functions of responding to drought stress and regulating the accumulation of tanshinones.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome sequencing and metabolome analysis reveal the molecular mechanism of Salvia miltiorrhiza in response to drought stress

Zhou,

Bai,

Han

et al. 2024

BMC Plant Biol

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Salvia miltiorrhiza is commonly used as a Chinese herbal medicine to treat different cardiovascular and cerebrovascular illnesses due to its active ingredients. Environmental conditions, especially drought stress, can affect the yield and quality of S. miltiorrhiza. However, moderate drought stress could improve the quality of S. miltiorrhiza without significantly reducing the yield, and the mechanism of this initial drought resistance is still unclear. In our study, transcriptome and metabolome analyses of S. miltiorrhiza under different drought treatment groups (CK, A, B, and C groups) were conducted to reveal the basis for its drought tolerance. We discovered that the leaves of S. miltiorrhiza under different drought treatment groups had no obvious shrinkage, and the malondialdehyde (MDA) contents as well as superoxide dismutase (SOD) and peroxidase (POD) activities dramatically increased, indicating that our drought treatment methods were moderate, and the leaves of S. miltiorrhiza began to initiate drought resistance. The morphology of root tissue had no significant change under different drought treatment groups, and the contents of four tanshinones significantly enhanced. In all, 5213, 6611, and 5241 differentially expressed genes (DEGs) were shared in the A, B, and C groups compared with the CK group, respectively. The results of KEGG and co-expression analysis showed that the DEGs involved in plant-pathogen interactions, the MAPK signaling pathway, phenylpropanoid biosynthesis, flavonoid biosynthesis, and plant hormone signal transduction responded to drought stress and were strongly correlated with tanshinone biosynthesis. Furthermore, the results of metabolism analysis indicated that 67, 72, and 92 differentially accumulated metabolites (DAMs), including fumarate, ferulic acid, xanthohumol, and phytocassanes, which were primarily involved in phenylpropanoid biosynthesis, flavonoid biosynthesis, and diterpenoid biosynthesis pathways, were detected in these groups. These discoveries provide valuable information on the molecular mechanisms by which S. miltiorrhiza responds to drought stress and will facilitate the development of drought-resistant and high-quality S. miltiorrhiza production.

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“…Chalcone synthase (CHS) is a crucial rate-limiting enzyme in the flavonoid biosynthetic pathway that catalyzes the condensation of malonyl-CoA and ρ-coumaroyl-CoA to produce naringenin chalcone, which serves as the precursor of a variety of flavonoid derivatives. These flavonoids are involved in the response to and protection of plants from abiotic and biotic stress, including ultraviolet radiation, temperature, humidity and pathogenic attack [ 37 , 38 ]…”

Section: Discussionmentioning

confidence: 99%

Genetic Analyses of Seed Longevity in Capsicum annuum L. in Cold Storage Conditions

Arif

Tripodi

Waheed

et al. 2023

Plants

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Seed longevity is the most important trait in the genebank management system. No seed can remain infinitely viable. There are 1241 accessions of Capsicum annuum L. available at the German Federal ex situ genebank at IPK Gatersleben. C. annuum (Capsicum) is the most economically important species of the genus Capsicum. So far, there is no report that has addressed the genetic basis of seed longevity in Capsicum. Here, we convened a total of 1152 Capsicum accessions that were deposited in Gatersleben over forty years (from 1976 to 2017) and assessed their longevity by analyzing the standard germination percentage after 5–40 years of storage at −15/−18 °C. These data were used to determine the genetic causes of seed longevity, along with 23,462 single nucleotide polymorphism (SNP) markers covering all of the 12 Capsicum chromosomes. Using the association-mapping approach, we identified a total of 224 marker trait associations (MTAs) (34, 25, 31, 35, 39, 7, 21 and 32 MTAs after 5-, 10-, 15-, 20-, 25-, 30-, 35- and 40-year storage intervals) on all the Capsicum chromosomes. Several candidate genes were identified using the blast analysis of SNPs, and these candidate genes are discussed.

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Genome-wide characterization of chalcone synthase genes in sweet cherry and functional characterization of CpCHS1 under drought stress

Cited by 23 publications

References 75 publications

HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd

HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd

Transcriptome sequencing and metabolome analysis reveal the molecular mechanism of Salvia miltiorrhiza in response to drought stress

Genetic Analyses of Seed Longevity in Capsicum annuum L. in Cold Storage Conditions

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