Genome-Wide Characterization of High-Affinity Nitrate Transporter 2 (NRT2) Gene Family in Brassica napus

Du, Runjie; Wu, Zexuan; Yu, Zhao-Xi; Li, Peng-Feng; Mu, Jian-Yu; Zhou, Jie; Li, Jiana; Du, Hongwu

doi:10.3390/ijms23094965

Cited by 11 publications

(9 citation statements)

References 62 publications

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“…Contrarily, NRT2 family plays an imperative role in NO 3 acquisition under N deficient conditions (Zou et al, 2020;Li et al, 2022b;Du et al, 2022). For instance, NO 3 transporter-like NRT2.1, NRT2.2, NRT2.4, and NRT2.5 require a partner protein, NAR2.1 (Kiba et al, 2012;Lezhneva et al, 2014;Zou et al, 2020) in Arabidopsis to acclimatize under such conditions.…”

Section: Gene Expression Action Referencesmentioning

confidence: 99%

“…For instance, NO 3 transporter-like NRT2.1, NRT2.2, NRT2.4, and NRT2.5 require a partner protein, NAR2.1 (Kiba et al, 2012;Lezhneva et al, 2014;Zou et al, 2020) in Arabidopsis to acclimatize under such conditions. In addition, other transporters such as CmNRT2.1 in Chrysanthemum indicum (Chrysanthemum; Gu et al, 2016), TaNRT2.1-6B in wheat (Li et al, 2022a), BnaZSNRT2s in Brassica napus (Canola; Du et al, 2022) and OsNRT2.1, OsNRT2.2, OsNRT2.4 and their partner protein OsNAR2.1 in rice (Naz et al, 2019;Souza et al, 2022) also function as NO 3 influx transporters (Figure 2). Among all NO 3 transporters, the dual affinity NO 3 transporter NRT1.1 (AtNPF6.3) serve as a multifunctional protein with a critical role in both NO 3 acquisition and sensing.…”

Section: Gene Expression Action Referencesmentioning

confidence: 99%

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Sustaining nitrogen dynamics: A critical aspect for improving salt tolerance in plants

et al. 2023

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In the current changing environment, salt stress has become a major concern for plant growth and food production worldwide. Understanding the mechanisms of how plants function in saline environments is critical for initiating efforts to mitigate the detrimental effects of salt stress. Agricultural productivity is linked to nutrient availability, and it is expected that the judicious metabolism of mineral nutrients has a positive impact on alleviating salt-induced losses in crop plants. Nitrogen (N) is a macronutrient that contributes significantly to sustainable agriculture by maintaining productivity and plant growth in both optimal and stressful environments. Significant progress has been made in comprehending the fundamental physiological and molecular mechanisms associated with N-mediated plant responses to salt stress. This review provided an (a) overview of N-sensing, transportation, and assimilation in plants; (b) assess the salt stress-mediated regulation of N dynamics and nitrogen use- efficiency; (c) critically appraise the role of N in plants exposed to salt stress. Furthermore, the existing but less explored crosstalk between N and phytohormones has been discussed that may be utilized to gain a better understanding of plant adaptive responses to salt stress. In addition, the shade of a small beam of light on the manipulation of N dynamics through genetic engineering with an aim of developing salt-tolerant plants is also highlighted.

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Section: Gene Expression Action Referencesmentioning

confidence: 99%

Section: Gene Expression Action Referencesmentioning

confidence: 99%

Sustaining nitrogen dynamics: A critical aspect for improving salt tolerance in plants

et al. 2023

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“…NRT2 homologous proteins are typical high-affinity nitrate transporters in the plant kingdom and are responsible for the nitrate uptake process in plants. In general, NRT2 proteins (NRT2s) have a typical membrane topology connected by a cytosolic loop, including 1 MFS domain that exhibits dual affinities for nitrate 18 and 12 transmembrane domains 19 , which are usually located on the cell plasma membrane 20 . Lots of NRT2 members have been identified in Nicotiana tabacum, Glycine max, Arabidopsis thaliana, Solanum lycopersicum, Oryza sativa, Zea mays, Triticum aestivum, and so on [21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of high-affinity nitrate transporter 2 (NRT2) gene family in alfalfa (Medicago sativa)

Luo,

Nan

2024

Preprint

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The high-affinity nitrate transporter 2 (NRT2) proteins plays an important role in nitrate uptake and transport in plants. In this study, the NRT2s gene family was systematically analyzed in alfalfa. We showed that a total of three NRT2 members were identified from the genome database, according to their chromosomal location, they were named MsNRT2.1-2.3. The phylogenetic tree revealed that NRT2 proteins were categorized into two main subgroups, which was further confirmed by their gene structure and conserved motifs. Three MsNRT2 genes distributed on 2 chromosomes. Furthermore, we studied the expression pattern of MsNRT2 gene in six tissues based on RNA-sequencing data from the Short Read Archive (SRA) database of NCBI, and the results showed that MsNRT2 gene was widely expressed in six tissues. After treatment with drought, salt, abscisic acid (ABA) and salicylic acid (SA), We used quantitative RT-PCR to analyze the expression levels of MsNRT2 genes and the results showed that most of the MsNRT2 genes responded to these stresses. However, the individual genes play a major role under the different treatment conditions. In summary, MsNRT2 plays an irreplaceable role in the growth, development and stress response of alfalfa, and this study provides valuable information and theoretical basis for future research on MsNRT2 function.

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“…Subsequently, numerous studies have investigated the functional roles of the plant NRT2 family and important progress has been made. It has been reported that there are 7 NRT2 genes in Arabidopsis [10,16], 4 in rice [17], 4 in maize [18], 31 in Brassica napus [19,20], 13 in poplar [21], 4 in tomato [22], and 5 in wild soybean (Glycine soja) [23]. In Arabidopsis, four AtNRT2 transporters (AtNRT2.1, AtNRT2.2, AtNRT2.3, and AtNRT2.4) are involved in nitrate uptake.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide systematic characterization of the NRT2 gene family and its expression profile in wheat (Triticum aestivum L.) during plant growth and in response to nitrate deficiency

Deng

Luo

Zheng

et al. 2023

Preprint

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Background: Wheat (Triticum aestivum L.) is a major crop that is grown worldwide, but it is highly dependent on sufficient N supply. The molecular mechanisms associated with nitrate uptake and assimilation are still poorly understood in wheat. In plants, NRT2 family proteins play a crucial role in NO3– acquisition and translocation under limited nitrate conditions. However, the biological functions of these genes in wheat are still unclear, especially their roles in NO3– uptake and assimilation. Results: In this study, a comprehensive analysis of wheat TaNRT2 genes was conducted using bioinformatics and molecular biology methods and 49 TaNRT2 genes were identified. A phylogenetic analysis clustered the TaNRT2 genes into three clades. The genes clustered on the same phylogenetic branch had similar gene structures and nitrate assimilation functions. The identified genes were further mapped onto the 13 wheat chromosomes and the results showed that a large duplication event had occurred on chromosome 6. To explore the TaNRT2 genes expression profiles in wheat, we performed transcriptome sequencing after low nitrate treatment for three days. The transcriptome analysis revealed that expression levels of all TaNRT2 genes in shoot and root and, based on the expression profiles, three highly expressed genes (TaNRT2-6A.2, TaNRT2-6A.6, and TaNRT2-6B.4) were selected for a qPCR analysis in two different wheat cultivars (‘Mianmai367’ and ‘Nanmai660’) under nitrogen limited and normal conditions. All three genes were up-regulated under nitrate limited conditions and highly expressed in the high nitrogen use efficiency (NUE) wheat ‘Mianmai36’ under low nitrate conditions. Conclusion: We systematically identified 49 NRT2 gens in wheat and analyzed the transcript levels of all the TaNRT2s under nitrogen deficiency conditions and over the whole growth period. These results suggest that these genes play important roles in nitrate absorption, distribution, and accumulation. This study provides valuable information and key candidate genes for further studies on the function TaNRT2s in wheat.

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Genome-Wide Characterization of High-Affinity Nitrate Transporter 2 (NRT2) Gene Family in Brassica napus

Cited by 11 publications

References 62 publications

Sustaining nitrogen dynamics: A critical aspect for improving salt tolerance in plants

Sustaining nitrogen dynamics: A critical aspect for improving salt tolerance in plants

Genome-wide identification of high-affinity nitrate transporter 2 (NRT2) gene family in alfalfa (Medicago sativa)

Genome-wide systematic characterization of the NRT2 gene family and its expression profile in wheat (Triticum aestivum L.) during plant growth and in response to nitrate deficiency

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