Genome-Wide Characterization of the C-repeat Binding Factor (CBF) Gene Family Involved in the Response to Abiotic Stresses in Tea Plant (Camellia sinensis)

Hu, Zhe; Ban, Qiuyan; Hao, Jing; Zhu, Xiangxiang; Cheng, Yaohua; Mao, Junlin; Lin, Mengling; Xia, Enhua; Li, Yeyun

doi:10.3389/fpls.2020.00921

Cited by 60 publications

(49 citation statements)

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“…Previous gene expression studies have reported that most AP2/ERFs are expressed at low levels under normal conditions, but their expression can be induced or repressed at certain growth stages by hormones and stress stimuli (Xie et al, 2019 ). The DREB subfamily may be a key candidate for future exploration of a means to enhance drought and cold tolerance in tea (Ban et al, 2017 ; Parmar et al, 2019 ; Wang et al, 2019 ; Hu et al, 2020 ). It has been classified into six subgroups (A1–A6) (Sakuma et al, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

“…Many transcription factors and metabolite-related genes have been shown to be involved in both the cold and drought responses of plants. For example, the key cold regulators ICE, CBF , and DHN transcription factors participate in both cold and drought and in other abiotic stresses (Liu et al, 2015 ; Liu S.-C. et al, 2016 ; Yin et al, 2016 ; Ban et al, 2017 ; Hu et al, 2020 ). The genes involved in the ABA-independent responsive pathway and the bZIP -mediated ABA-dependent pathway (Wang et al, 2012 ; Ban et al, 2017 ) also participate in tolerance to cold and drought.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

et al. 2020

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Cold and drought are two of the most severe threats affecting the growth and productivity of the tea plant, limiting its global spread. Both stresses cause osmotic changes in the cells of the tea plant by decreasing their water potential. To develop cultivars that are tolerant to both stresses, it is essential to understand the genetic responses of tea plant to these two stresses, particularly in terms of the genes involved. In this study, we combined literature data with interspecific transcriptomic analyses (using Arabidopsis thaliana and Solanum lycopersicum) to choose genes related to cold tolerance. We identified 45 stress-inducible candidate genes associated with cold and drought responses in tea plants based on a comprehensive homologous detection method. Of these, nine were newly characterized by us, and 36 had previously been reported. The gene network analysis revealed upregulated expression in ICE1-related cluster of bHLH factors, HSP70/BAM5 connected genes (hexokinases, galactinol synthases, SnRK complex, etc.) indicating their possible co-expression. Using qRT-PCR we revealed that 10 genes were significantly upregulated in response to both cold and drought in tea plant: HSP70, GST, SUS1, DHN1, BMY5, bHLH102, GR-RBP3, ICE1, GOLS1, and GOLS3. SnRK1.2, HXK1/2, bHLH7/43/79/93 were specifically upregulated in cold, while RHL41, CAU1, Hydrolase22 were specifically upregulated in drought. Interestingly, the expression of CIP was higher in the recovery stage of both stresses, indicating its potentially important role in plant recovery after stress. In addition, some genes, such as DHN3, bHLH79, PEI54, SnRK1.2, SnRK1.3, and Hydrolase22, were significantly positively correlated between the cold and drought responses. CBF1, GOLS1, HXK2, and HXK3, by contrast, showed significantly negative correlations between the cold and drought responses. Our results provide valuable information and robust candidate genes for future functional analyses intended to improve the stress tolerance of the tea plant and other species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

et al. 2020

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“…CBF 4 expression is more activated by the exogenous application of ABA in Arabidopsis and grapevine. CBF 1 − 3 expression was also enhanced with the ABA external applications in main part due to the increased activity of CBF promoters in reply to ABA 54,56,57 . The differences in CBF proteins in grapevine re ect the positioning of CBF 4 in the separate phylogenetic groups compared to CBF 1,2, 3 19,58,59 .…”

Section: Discussionmentioning

confidence: 99%

Salinity Depression in Vitro Impress the Growth Potential and, Mediates the Antioxidants Pool and the Tolerance Genes Expression of Grapevine

Aazami

Hassanpouraghdam

2020

Preprint

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Undoubtedly, salinity is the major environmental stress affecting the crops growth, development and yield. Grapevine is a dominant horticulture crop and mildly sensitive to the salinity. Salinity triggers variations at the cellular and molecular levels and hence induces some specific genes expression. The responses of two grapevine cultivars (Fakhri and Sultanin) were evaluated for its tolerance to the salinity stress in vitro. The results for the explants exposed to the salinity revealed that the viability, fresh weight of the regenerated explant and the proliferation rate were declined compared to the control ones. The activity of SOD enzyme and MDA content were increased with salinity. However, protein content declined. There was no significant difference in CAT and APX activities with the salinity treatment. With salinity adding up, the DREB/CBFs genes expression pattern was significantly increased in both cultivars. ‘Fakhri’ was more responsive in growth parameters and the activity of antioxidant enzymes and higher expression rate of DREB/CBFs under salinity compared to the variety ‘Sultanin’.

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“…The genes involved in the ABA-independent responsive pathway and the bZIP -mediated ABA-dependent pathway participate in tolerance to osmotic stress induced by drought and cold [ 22 ]. In tea plants, 12 major TF-families are believed to be involved in the osmotic stress response viz., AP2/EREBP, bHLH, bZIP, HD-ZIP, HSF (HSP), MYB, NAC, WRKY, zinc-finger protein TFs, SCL, ARR, and SPL [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. Many sugar metabolism genes were also induced in response to osmotic stress in tea [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

Самарина

Matskiv

Simonyan

et al. 2020

Plants

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Osmotic stress is a major factor reducing the growth and yield of many horticultural crops worldwide. To reveal reliable markers of tolerant genotypes, we need a comprehensive understanding of the responsive mechanisms in crops. In vitro stress induction can be an efficient tool to study the mechanisms of responses in plants to help gain a better understanding of the physiological and genetic responses of plant tissues against each stress factor. In the present study, the osmotic stress was induced by addition of mannitol into the culture media to reveal biochemical and genetic responses of tea microplants. The contents of proline, threonine, epigallocatechin, and epigallocatechin gallate were increased in leaves during mannitol treatment. The expression level of several genes, namely DHN2, LOX1, LOX6, BAM, SUS1, TPS11, RS1, RS2, and SnRK1.3, was elevated by 2–10 times under mannitol-induced osmotic stress, while the expression of many other stress-related genes was not changed significantly. Surprisingly, down-regulation of the following genes, viz. bHLH12, bHLH7, bHLH21, bHLH43, CBF1, WRKY2, SWEET1, SWEET2, SWEET3, INV5, and LOX7, was observed. During this study, two major groups of highly correlated genes were observed. The first group included seven genes, namely CBF1, DHN3, HXK2,SnRK1.1, SPS, SWEET3, and SWEET1. The second group comprised eight genes, viz. DHN2, SnRK1.3, HXK3, RS1, RS2,LOX6, SUS4, and BAM5. A high level of correlation indicates the high strength connection of the genes which can be co-expressed or can be linked to the joint regulons. The present study demonstrates that tea plants develop several adaptations to cope under osmotic stress in vitro; however, some important stress-related genes were silent or downregulated in microplants.

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Genome-Wide Characterization of the C-repeat Binding Factor (CBF) Gene Family Involved in the Response to Abiotic Stresses in Tea Plant (Camellia sinensis)

Abstract: Genome-Wide Characterization of the C-repeat Binding Factor (CBF) Gene Family Involved in the Response to Abiotic Stresses in Tea Plant (Camellia sinensis). Front. Plant Sci. 11:921.

Cited by 60 publications

References 62 publications

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

Salinity Depression in Vitro Impress the Growth Potential and, Mediates the Antioxidants Pool and the Tolerance Genes Expression of Grapevine

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

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