Genome-wide characterization of the laccase gene family in Setaria viridis reveals members potentially involved in lignification

Simões, Marcella Siqueira; Carvalho, Gabriel Garon; Ferreira, Sávio Siqueira; Hernandes‐Lopes, José; Setta, Nathalia de; Cesarino, Igor

doi:10.1007/s00425-020-03337-x

Cited by 37 publications

(28 citation statements)

References 65 publications

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“…Laccases are a large group of multicopper oxidases that are widely distributed in bacterial, fungi, animals, and plants. Recent advances in high-throughput sequencing technology and molecular biology have allowed several laccases that participate in lignin polymerization to be characterized in multiple plant species (Cheng et al, 2019;He et al, 2019;Le Bris et al, 2019;Wang et al, 2019;Simões et al, 2020).…”

Section: Chlac8 Expression Parallels C-lignin Accumulation In the Clementioning

confidence: 99%

Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana

et al. 2020

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Section: Chlac8 Expression Parallels C-lignin Accumulation In the Clementioning

confidence: 99%

Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana

et al. 2020

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“…For the expression analysis, induced and control cells were harvested by vacuum filtration at 0, 12 h, 48 h, and 1 week of treatment, frozen in liquid nitrogen and ground into a fine powder. Total RNA isolation, cDNA synthesis and RT-qPCR analysis were performed as previously described (Simões et al, 2020). Relative expression of each gene was calculated by normalizing it with the geometric mean of the relative quantities of three housekeeping genes, POLYUBIQUITIN (ScPUB), UBIQUITIN-CONJUGATING ENZYME 2 (ScUBE2), and GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (ScGAPDH) (Ferreira et al, 2016).…”

Section: Rt-qpcr Expression Analysismentioning

confidence: 99%

“…Lignin polymerization occurs in the apoplast and is mediated by laccases and class III peroxidases that are responsible for the oxidation of lignin monomers. These enzymes belong to large multigene families with highly redundant members and diverse functions, making the identification of individual genes involved in lignification a difficult task (Shigeto and Tsutsumi, 2016;Simões et al, 2020). Thus, genes encoding laccases and peroxidases that are specifically upregulated during the differentiation of TEs in the sugarcane culture are strong candidates to be involved in lignin polymerization.…”

Section: Scw-related Genes Are Upregulated During the Differentiationmentioning

confidence: 99%

Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming

et al. 2020

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Plant lignocellulosic biomass, mostly composed of polysaccharide-rich secondary cell walls (SCWs), provides fermentable sugars that may be used to produce biofuels and biomaterials. However, the complex chemical composition and physical structure of SCWs hinder efficient processing of plant biomass. Understanding the molecular mechanisms underlying SCW deposition is, thus, essential to optimize bioenergy feedstocks. Here, we establish a xylogenic culture as a model system to study SCW deposition in sugarcane; the first of its kind in a C4 grass species. We used auxin and brassinolide to differentiate sugarcane suspension cells into tracheary elements, which showed metaxylem-like reticulate or pitted SCW patterning. The differentiation led to increased lignin levels, mainly caused by S-lignin units, and a rise in p-coumarate, leading to increased p-coumarate:ferulate ratios. RNAseq analysis revealed massive transcriptional reprogramming during differentiation, with upregulation of genes associated with cell wall biogenesis and phenylpropanoid metabolism and downregulation of genes related to cell division and primary metabolism. To better understand the differentiation process, we constructed regulatory networks of transcription factors and SCW-related genes based on co-expression analyses. Accordingly, we found multiple regulatory modules that may underpin SCW deposition in sugarcane. Our results provide important insights and resources to identify biotechnological strategies for sugarcane biomass optimization.

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“…Because of the functional redundancy of laccase genes, single suppressed gene has little influence on the plant phenotypes (Simoes et al, 2020). Therefore, it is necessary to silence or knockout multiple genes in the laccase gene family to comprehensively evaluate their roles.…”

mentioning

confidence: 99%

Multiplexed CRISPR/Cas9-Mediated Knockout of Laccase Genes in Salvia miltiorrhiza Revealed Their Roles in Growth, Development, and Metabolism

Zhou

Liang

et al. 2021

Front. Plant Sci.

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Laccases are multicopper-containing glycoproteins related to monolignol oxidation and polymerization. These properties indicate that laccases may be involved in the formation of important medicinal phenolic acid compounds in Salvia miltiorrhiza such as salvianolic acid B (SAB), which is used for cardiovascular disease treatment. To date, 29 laccases have been found in S. miltiorrhiza (SmLACs), and some of which (SmLAC7 and SmLAC20) have been reported to influence the synthesis of phenolic acids. Because of the functional redundancy of laccase genes, their roles in S. miltiorrhiza are poorly understood. In this study, the CRISPR/Cas9 system was used for targeting conserved domains to knockout multiple genes of laccase family in S. miltiorrhiza. The expressions of target laccase genes as well as the phenolic acid biosynthesis key genes decrease dramatically in editing lines. Additionally, the growth and development of hairy roots was significantly retarded in the gene-edited lines. The cross-sections examination of laccase mutant hairy roots showed that the root development was abnormal and the xylem cells in the edited lines became larger and looser than those in the wild type. Additionally, the accumulation of RA as well as SAB was decreased, and the lignin content was nearly undetectable. It suggested that SmLACs play key roles in development and lignin formation in the root of S. miltiorrhiza and they are necessary for phenolic acids biosynthesis.

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Genome-wide characterization of the laccase gene family in Setaria viridis reveals members potentially involved in lignification

Cited by 37 publications

References 65 publications

Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana

Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana

Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming

Multiplexed CRISPR/Cas9-Mediated Knockout of Laccase Genes in Salvia miltiorrhiza Revealed Their Roles in Growth, Development, and Metabolism

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