Genome-wide computational determination of the human metalloproteome

Azia, Ariel; Levy, Ronen; Unger, Ron; Edelman, Marvin; Sobolev, V. M.

doi:10.1002/prot.24790

Cited by 13 publications

(10 citation statements)

References 46 publications

(82 reference statements)

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“…Metal ions are needed by the human body and frequently serve as cofactors in enzymatic reactions and in maintaining protein structure. One-third of human proteins bind with metal ions, and over 10% of enzymes in the human body require Zn for activity (Azia, Levy, Unger, Edelman, & Sobolev, 2015). Iron is a vital trace element in the human body, controlling infection and cell-mediated immunity (Bhashkaran, 2001).…”

Section: Mineral Composition Analysismentioning

confidence: 99%

Nutritional Composition of the Green Leaves of Quinoa (Chenopodium quinoa Willd.)

Pathan¹,

Eivazi²,

Valliyodan³

et al. 2019

JFR

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Quinoa (Chenopodium quinoa Willd.) grain is often eaten worldwide as a healthy food, but consuming nutrient-rich quinoa leaves as a leafy green vegetable is uncommon. This study evaluated the potentiality of leafy green quinoa as a major source of protein, amino acids, and minerals in the human diet. Also, the study compared the nutrient content of quinoa leaves with those of amaranth and spinach leaves. The proximate analysis of quinoa dry leaves showed a higher amount (g/100 g dry weight) of protein (37.05) than amaranth (27.45) and spinach (30.00 g). Furthermore, a lower amount of carbohydrate (34.03) was found in quinoa leaves compared to amaranth (47.90) and spinach (43.78 g). A higher amount of essential amino acids was found in quinoa leaves relative to those of amaranth and spinach. The highest amounts (mg/100 g dry weight) of minerals in quinoa dry leaves were copper (1.12), manganese (26.49), and potassium (8769.00 mg), followed by moderate amounts of calcium (1535.00), phosphorus (405.62), sodium (15.12), and zinc (6.79 mg). Our findings suggest that quinoa leaves can be consumed as a green vegetable with an excellent source of nutrients. Therefore, we endorse the inclusion of quinoa in the leafy green vegetable group.

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Section: Mineral Composition Analysismentioning

confidence: 99%

Nutritional Composition of the Green Leaves of Quinoa (Chenopodium quinoa Willd.)

Pathan¹,

Eivazi²,

Valliyodan³

et al. 2019

JFR

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“…One third of the body’s proteins bind to metal ions, requiring approximately 10% of the enzymes in our body zinc for its activity [44]. Zinc deficiency contributes to the death of 800,000 children worldwide per year [45].…”

Section: Discussionmentioning

confidence: 99%

Ethnobotanical and nutritional study ofquelitessold in two traditional markets of Oaxaca, Mexico

Medina

Pérez‐Herrera

Lustre-Sánchez

et al. 2018

Preprint

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SUMMARYBackground.In Mexico, it is called quelites to certain edible vegetables (young plants, germ, shoots or flowers). Since pre-Hispanic times, quelites have been eaten as a source of vitamins, minerals and proteins. Now, its traditional and healthy consumption has decreased. We studied the quelites of two traditional markets in the Valles Centrales of Oaxaca state, Mexico using an ethnobotanical and nutritional approach.MethodsFrom July 2017 to July 2018, weekly ethnobotanical interviews were conducted with 26 collectors-sellers of the Zimatlán market and 36 in the Zaachila market. The vegetal supply was acquired, herborized and identified by through dichotomous keys. There were determined the proximal composition, phenolic compounds, flavonoids, antioxidant capacity and mineral content of the floral structures of two quelites’ types. The statistical analysis was performed through a one-way analysis of variance (ANOVA) of Tukey HSD.ResultsIn two sampled markets, 23 species belonging to 11 botanical families were registered, from which leaves, branches, stems, flowers and fruits are eaten. The flowers of the species Diphysa americana (Q1) and Phaseolus coccineus (Q2) are the most used for human consumption of the communities involved in the sale of the sampled quelites. Both flowers had important amounts of proteins (2.66-3.29%) and fiber (1.66-2.43%). Q1 had higher content of phenols and flavonoids and therefore higher antioxidant capacity than Q2 (p <0.05). When we talk about Q2 minerals, it presented a greater amount of Zn, Ca and Mg in comparison to Q1 (p> 0.05).ConclusionsIn local markets of the state of Oaxaca, a wide variety of quelites are usually found, where their botanical structures, such as flowers, are widely eaten. The flowers of Q1 and Q2 proved to be a rich source of proteins and bioactive compounds, as well as minerals. Showing thus to be a food alternative to enrich the human diet.

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“…Metal ions are particularly suitable for controlling conformational changes due to their ubiquitous role in various biological systems . Zinc is one of the most prevalent protein‐binding metals as it is found in more than half of all known human metalloproteins . Due to the high occurrence of Zn II binding sites in proteins, the preferred binding residues and coordination geometry are well understood, which has enabled elucidation of principles for designing metalloproteins .…”

Section: Methodsmentioning

confidence: 99%

SwitCCh: Metal‐Site Design for Controlling the Assembly of a Coiled‐Coil Homodimer

2018

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Conformational change of proteins in response to chemical or physical signals is the underlying principle of many regulatory and transport mechanisms in biological systems. The ability to design proteins the conformational state of which can be precisely and reversibly controlled would facilitate the development of molecular machines tailored for specific applications. Here we explore metal‐binding site design to engineer a peptide‐based conformational switch called SwitCCh that assembles into a homodimeric coiled‐coil in response to the addition of ZnII ions or low pH. Addition of ZnII promoted formation of a parallel homodimer with an increase in thermal stability by more than 30 °C. The peptide could be reversibly cycled between the coiled‐coil and random conformation. Furthermore, the SwitCCh peptide was orthogonal to the previously developed coiled‐coil dimer set, indicating it could be used for regulated self‐assembly of coiled‐coil based nanostructures and materials.

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Genome-wide computational determination of the human metalloproteome

Cited by 13 publications

References 46 publications

Nutritional Composition of the Green Leaves of Quinoa (Chenopodium quinoa Willd.)

Nutritional Composition of the Green Leaves of Quinoa (Chenopodium quinoa Willd.)

Ethnobotanical and nutritional study ofquelitessold in two traditional markets of Oaxaca, Mexico

SwitCCh: Metal‐Site Design for Controlling the Assembly of a Coiled‐Coil Homodimer

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