Genome-wide differential expression of genes and small RNAs in testis of two different porcine breeds and at two different ages

Yao, Li; Li, Jialian; Cui, Fang; Shi, Liang; Tan, Jinyun; Xiong, Yan; Fan, Bin; Li, Changchun

doi:10.1038/srep26852

Cited by 31 publications

(27 citation statements)

References 94 publications

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“…In addition, after identifying the miRNA targets of each differentially regulated circRNA, we found that some candidate miRNAs targeted by several circRNAs are linked to mammalian development of sex differentiation and maturation. For example, miR-145-5p was found likewise up-regulated after sexual maturity in pigs (Li et al, 2016) and miR-214-3p was shown to be involved in the onset of mouse primordial germ and somatic cell sex differentiation (Fernández-Pérez et al, 2018). Those findings coupled to our results suggest that circRNAs probably regulate the onset of puberty.…”

Section: Discussionsupporting

confidence: 82%

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

et al. 2020

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Being the center of the hypothalamus-pituitary-ovary (HPO) axis, the pituitary plays a key role in the onset of puberty. Recent studies show that circular RNAs (circRNAs) can perform as miRNA sponges to regulate development in animals. However, the function of pituitary-derived circRNAs in first estrus remains unclear in pigs. In this study, we performed a genome-wide identification and characterization of circRNAs using pituitaries from Landrace × Yorkshire crossbred pigs at three stages: pre-, in-, and post-puberty, to describe such pituitary-derived circRNAs in pigs. A total of 5148 circRNAs were found in the gilts' pituitaries, averaging 18 682 bp in genomic distance, which consisted of approximately 91% exonic, 6% intergenic, and 3% intronic circRNAs. Furthermore, 158 novel circRNAs were identified for the first time and classified as putative pituitary-specific circRNAs. Their expression levels during the onset of puberty, significantly exceeded those of the other circRNAs, and the parental genes of these putative pituitary-specific circRNAs were enriched in "ssc04917: prolactin signaling pathway," "ssc04080: neuroactive ligand-receptor interaction," and "ssc04728: dopaminergic synapse" pathways, all of which were consistent with pituitary functioning. Additionally, 17 differentially regulated circRNAs were found and investigated for their potential interaction with miRNAs, along with genes, by constructing a circRNA-targeted miRNA-gene network. Taken together, these results provide new insight into the circRNA-mediated timing of puberty in gilts at the pituitary level.

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Section: Discussionsupporting

confidence: 82%

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

et al. 2020

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“…However, hundreds of mature miRNAs have been detected in developing porcine testicular tissues. Therefore, further studies are needed to explore the functional roles of miRNAs in porcine Sertoli cells ( Luo et al, 2015 , 2018 ; Li et al, 2016 ; Xu et al, 2018 ). In the present study, we report that miR-222 inhibited cell cycle and proliferation and induced cell apoptosis in immature Sertoli cells by targeting the GRB10 gene through the inactivation of the PI3K/AKT signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

miR-222 Suppresses Immature Porcine Sertoli Cell Growth by Targeting the GRB10 Gene Through Inactivating the PI3K/AKT Signaling Pathway

et al. 2020

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Sertoli cells are central and essential coordinators of spermatogenesis. Accumulating evidence has demonstrated that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and the regulatory mechanisms of miRNAs in Sertoli cells of domestic animals remain largely unknown. Here we report that miR-222 overexpression repressed cell cycle progression and proliferation and promoted the apoptosis of immature porcine Sertoli cells, whereas miR-222 inhibition resulted in the opposite result. miR-222 directly targeted the 3′-UTR of the GRB10 gene and inhibited its mRNA abundance. An siRNA-induced GRB10 knockdown showed similar effects as did miR-222 overexpression on cell proliferation and apoptosis and further attenuated the role of miR-222 inhibition. Furthermore, both miR-222 overexpression and GRB10 inhibition repressed the phosphorylation of PI3K and AKT, the key elements of the PI3K/AKT signaling pathway, whereas GRB10 inhibition offsets the effects of the miR-222 knockdown. Overall, we concluded that miR-222 suppresses immature porcine Sertoli cell growth by targeting the GRB10 gene through inactivation of the PI3K/AKT signaling pathway. This study provides novel insights into the epigenetic regulation of porcine spermatogenesis by determining the fate of Sertoli cells.

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“…miR‐26a was widely identified in porcine testicular tissues using RNA‐seq technology (Li, Li, et al., ; Lian et al., ; Luo et al., ; Ran et al., ), suggesting its key roles in swine testicular development and spermatogenesis. Although previous studies reported that miR‐26a plays multiple regulatory roles in cell metabolic processes, including differentiation (Su et al., ), proliferation (Zhou et al., ) and apoptosis (Zhang et al., ).…”

Section: Discussionmentioning

confidence: 99%

miR‐26a suppresses autophagy in swine Sertoli cells by targeting ULK2

Ran

Cao

et al. 2018

Reprod Domestic Animals

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A large number of microRNAs (miRNAs) have been detected from porcine testicular tissues thanks to the development of high-throughput sequencing technology. However, the regulatory roles of most identified miRNAs in swine testicular development or spermatogenesis are poorly understood. In our previous study, ULK2 (uncoordinated-51-like kinase 2) was predicted as a target gene of miR-26a. In this study, we aimed to investigate the role of miR-26a in swine Sertoli cell autophagy. The relative expression of miR-26a and ULK2 levels has a significant negative correlation (R = .5964, p ≤ .01) in nine developmental stages of swine testicular tissue. Dual-luciferase reporter assay results show that miR-26a directly targets the 3'UTR of the ULK2 gene (position 618-624). In addition, both the mRNA and protein expression of ULK2 were downregulated by miR-26a in swine Sertoli cells. These results indicate that miR-26a targets the ULK2 gene and downregulates its expression in swine Sertoli cells. Based on the expression of marker genes (LC3, p62 and Beclin-1), overexpression of miR-26a or knock-down of ULK2 inhibits swine Sertoli cell autophagy. Taken together, these findings demonstrate that miR-26a suppresses autophagy in swine Sertoli cells by targeting ULK2.

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Genome-wide differential expression of genes and small RNAs in testis of two different porcine breeds and at two different ages

Cited by 31 publications

References 94 publications

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

miR-222 Suppresses Immature Porcine Sertoli Cell Growth by Targeting the GRB10 Gene Through Inactivating the PI3K/AKT Signaling Pathway

miR‐26a suppresses autophagy in swine Sertoli cells by targeting ULK2

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