Genome-wide expression profiling of aquaporin genes confer responses to abiotic and biotic stresses in Brassica rapa

Kayum, Md. Abdul; Park, Jong-In; Nath, Ujjal Kumar; Biswas, Manosh Kumar; Kim, Hoy‐Taek; Nou, Ill–Sup

doi:10.1186/s12870-017-0979-5

Cited by 70 publications

(53 citation statements)

References 72 publications

(94 reference statements)

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“…Gene-specific primers of Brassica rapa L. ssp. pekinensis genes [43] were used for different PIP isoforms. The primers and the lengths of the amplicons are described in Supplementary Table S1.…”

Section: Quantitative Real-time Pcr (Qrt-pcr) Analysesmentioning

confidence: 99%

“…B. rapa var. italica, 18s ribosomal RNA [43] was used as the reference gene for standardisation of each sample. After denaturation at 95 • C for 10 min, amplification occurred in a two-step procedure: 15 s of denaturation at 95 • C and 1 min of annealing and extension at 60 • C for 40 cycles, followed by a dissociation stage.…”

Section: Quantitative Real-time Pcr (Qrt-pcr) Analysesmentioning

confidence: 99%

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Differential Aquaporin Response to Distinct Effects of Two Zn Concentrations after Foliar Application in Pak Choi (Brassica rapa L.) Plants

et al. 2020

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Zinc (Zn) is considered an essential element with beneficial effects on plant cells; however, as a heavy metal, it may induce adverse effects on plants if its concentration exceeds a threshold. In this work, the effects of short-term and prolonged application of low (25 µM) and high (500 µM) Zn concentrations on pak choi (Brassica rapa L.) plants were evaluated. For this, two experiments were conducted. In the first, the effects of short-term (15 h) and partial foliar application were evaluated, and in the second a long-term (15 day) foliar application was applied. The results indicate that at short-term, Zn may induce a rapid hydraulic signal from the sprayed leaves to the roots, leading to changes in root hydraulic conductance but without effects on the whole-leaf gas exchange parameters. Root accumulation of Zn may prevent leaf damage. The role of different root and leaf aquaporin isoforms in the mediation of this signal is discussed, since significant variations in PIP1 and PIP2 gene expression were observed. In the second experiment, low Zn concentration had a beneficial effect on plant growth and specific aquaporin isoforms were differentially regulated at the transcriptional level in the roots. By contrast, the high Zn concentration had a detrimental effect on growth, with reductions in the root hydraulic conductance, leaf photosynthesis rate and Ca2+ uptake in the roots. The abundance of the PIP1 isoforms was significantly increased during this response. Therefore, a 25 µM Zn dose resulted in a positive effect in pak choi growth through an increased root hydraulic conductance.

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Section: Quantitative Real-time Pcr (Qrt-pcr) Analysesmentioning

confidence: 99%

Section: Quantitative Real-time Pcr (Qrt-pcr) Analysesmentioning

confidence: 99%

Differential Aquaporin Response to Distinct Effects of Two Zn Concentrations after Foliar Application in Pak Choi (Brassica rapa L.) Plants

et al. 2020

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“…Many PIP2s have been reported to play roles in the drought response [41][42][43][44][45]. To understand the biological function of MtPIP2;7, we generated MtPIP2;7 knockout mutants using TALEN technology (Fig.…”

Section: The Aquaporin Mtpip2;7 Interacts With Mtcas31 and Plays A Nementioning

confidence: 99%

“…PM [46][47][48] and have been reported to participate in abiotic stress responses [40,45,58,59]. Overexpression of PIP2s can improve water use efficiency and enhance drought tolerance in rice, soybean and barley [42,46,59].…”

Section: Aquaporins Can Be Degraded Via Different Pathways Under Droumentioning

confidence: 99%

Dehydrin MtCAS31 promotes autophagic degradation under drought stress

Liu

Feng

et al. 2019

Autophagy

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Drought stress seriously affects crop yield, and the mechanism underlying plant resistance to drought stress via macroautophagy/autophagy is not clear. Here, we show that a dehydrin, Medicago truncatula MtCAS31 (cold acclimation-specific 31), a positive regulator of drought response, plays a key role in autophagic degradation. A GFP cleavage assay and treatment with an autophagy-specific inhibitor indicated that MtCAS31 participates in the autophagic degradation pathway and that overexpressing MtCAS31 promotes autophagy under drought stress. Furthermore, we discovered that MtCAS31 interacts with the autophagy-related protein ATG8a in the AIM-like motif YXXXI, supporting its function in autophagic degradation. In addition, we identified a cargo protein of MtCAS31, the aquaporin MtPIP2;7, by screening an M. truncatula cDNA library. We found that MtPIP2;7 functions as a negative regulator of drought response. Under drought stress, MtCAS31 facilitated the autophagic degradation of MtPIP2;7 and reduced root hydraulic conductivity, thus reducing water loss and improving drought tolerance. Taken together, our results reveal a novel function of dehydrins in promoting the autophagic degradation of proteins, which extends our knowledge of the function of dehydrins.

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“…PIP isoforms transporting water are relevant to osmotic hydraulic conductance, whereas members transporting CO 2 regulate stomatal and mesophyll conductance ( Pawlowicz and Masajada, 2019 ). PIP genes are associated with the abilities to tolerate several stress conditions such as salt, cold, and dehydration ( Kayum et al., 2017 ; Sun et al., 2017 ). Different PIPs can either play positive or negative roles in drought tolerance ( Hussain et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Physiological and PIP Transcriptional Responses to Progressive Soil Water Deficit in Three Mulberry Cultivars

Cao

Shen

et al. 2020

Front. Plant Sci.

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Although mulberry cultivars Wubu, Yu711, and 7307 display distinct anatomical, morphological, and agronomic characteristics under natural conditions, it remains unclear if they differ in drought tolerance. To address this question and elucidate the underlying regulatory mechanisms at the whole-plant level, 2-month old saplings of the three mulberry cultivars were exposed to progressive soil water deficit for 5 days. The physiological responses and transcriptional changes of PIPs in different plant tissues were analyzed. Drought stress led to reduced leaf relative water content (RWC) and tissue water contents, differentially expressed PIPs , decreased chlorophyll and starch, increased soluble sugars and free proline, and enhanced activities of antioxidant enzymes in all plant parts of the three cultivars. Concentrations of hydrogen peroxide (H 2 O 2 ), superoxide anion (O 2 •− ), and malonaldehyde (MDA) were significantly declined in roots, stimulated in leaves but unaltered in wood and bark. In contrast, except the roots of 7307, soluble proteins were repressed in roots and leaves but induced in wood and bark of the three cultivars in response to progressive water deficit. These results revealed tissue-specific drought stress responses in mulberry. Comparing to cultivar Yu711 and 7307, Wubu showed generally slighter changes in leaf RWC and tissue water contents at day 2, corresponding well to the steady PIP transcript levels, foliar concentrations of chlorophyll, O 2 •− , MDA, and free proline. At day 5, Wubu sustained higher tissue water contents in green tissues, displayed stronger responsiveness of PIP transcription, lower concentrations of soluble sugars and starch, lower foliar MDA, higher proline and soluble proteins, higher ROS accumulation and enhanced activities of several antioxidant enzymes. Our results indicate that whole-plant level responses of PIP transcription, osmoregulation through proline and soluble proteins and antioxidative protection are important mechanisms for mulberry to cope with drought stress. These traits play significant roles in conferring the relatively higher drought tolerance of cultivar Wubu and could be potentially useful for future mulberry improvement programmes.

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Genome-wide expression profiling of aquaporin genes confer responses to abiotic and biotic stresses in Brassica rapa

Cited by 70 publications

References 72 publications

Differential Aquaporin Response to Distinct Effects of Two Zn Concentrations after Foliar Application in Pak Choi (Brassica rapa L.) Plants

Differential Aquaporin Response to Distinct Effects of Two Zn Concentrations after Foliar Application in Pak Choi (Brassica rapa L.) Plants

Dehydrin MtCAS31 promotes autophagic degradation under drought stress

Physiological and PIP Transcriptional Responses to Progressive Soil Water Deficit in Three Mulberry Cultivars

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