Genome-wide identification and characterization of ACBP gene family in Populus reveal salinity alkali-responsive profiles

Abstract

Acyl-CoA-binding proteins (ACBPs) are important for the transport of acyl groups for macromolecular biosynthesis involved in plant growth, development, and diverse stress (e.g., cold, drought, salinity, and heavy metals) responses. Here, we report the phylogeny and characteristics of the ACBP family in the woody plant Populus trichocarpa. Eight genes encoding ACBP proteins were identified, and they are distributed on eight chromosomes in P. trichocarpa. These PtACBP genes were divided into four subgroups according to gene structure, conserved motifs and phylogenetic relationship. Promoter analysis revealed that cis-elements were related to stress response, phytohormone response, and physical and reproductive growth regulation. Expression levels of PtACBP genes varied among different organs, with the highest expression in leaves and the lowest in stems. Quantitative real-time PCR (qRT-PCR) analysis showed that under salinity-alkali stresses (i.e., 200 mM NaCl, 75 mM Na2CO3, and 100 mM NaHCO3), four (PtACBP1, PtACBP3, PtACBP4 and PtACBP8) of eight PtACBP genes were significantly induced in roots and leaves. These data provide a comprehensive analysis of the ACBPs family in P. trichocarpa, which could be useful for gene function analyses.

Genetic diversity and local adaption of alfalfa populations (Medicago sativa L.) under long-term grazing

“…The WD40 related proteins are involved in plant resistance to alkali stress 84 . The genes encoding ankyrin repeat domain has been reported to be associated with salinity-alkali stresses 85 . Our results showed that the LD distance within the alfalfa populations with long-term grazing history was larger (r 2 reduced to 0.2 after 3 cM) than previous studies 24 , 86 , 87 .…”

Studying the impact of titanium dioxide nanoparticles on the expression of pivotal genes related to menthol biosynthesis and certain biochemical parameters in peppermint plants (Mentha Piperita L.)

“…Research indicates that titanium promotes growth stimulation and facilitates the absorption of crucial elements like calcium, nitrogen, magnesium, manganese, phosphorus, iron, and zinc [ 13 ]. These intracellular alterations initiate diverse signaling pathways to translate physical stress into an appropriate biochemical response, each impacting the expression of stress-responsive genes [ 14 ]. The activation of these pathways facilitates plant adaptation and enhances its resilience against stressors [ 15 ].…”