Genome-wide identification and characterization of flavonol synthase (FLS) gene family in Brassica vegetables and their roles in response to biotic and abiotic stresses

Yue, Lingqi; Zhong, Min; Kang, Dengjin; Qin, Hongyi; Li, Haichou; Chai, Xirong; Kang, Yunyan; Yang, Xian

doi:10.1016/j.scienta.2024.113168

Scientia Horticulturae

2024

DOI: 10.1016/j.scienta.2024.113168

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Genome-wide identification and characterization of flavonol synthase (FLS) gene family in Brassica vegetables and their roles in response to biotic and abiotic stresses

Lingqi Yue,

Min Zhong,

Dengjin Kang

et al.

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Cited by 3 publications

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Metabolomics and transcriptomics analysis revealed the response mechanism of alfalfa to combined cold and saline‐alkali stress

Liu,

Si,

Zhang

et al. 2024

The Plant Journal

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SUMMARYCold and saline‐alkali stress are frequently encountered by plants, and they often occur simultaneously in saline‐alkali soils at mid to high latitudes, constraining forage crop distribution and production. However, the mechanisms by which forage crops respond to the combination of cold and saline‐alkali stress remain unknown. Alfalfa (Medicago sativa L.) is one of the most essential forage grasses in the world. In this study, we analyzed the complex response mechanisms of two alfalfa species (Zhaodong [ZD] and Blue Moon [BM]) to combined cold and saline‐alkali stress using multi‐omics. The results revealed that ZD had a greater ability to tolerate combined stress than BM. The tricarboxylic acid cycles of the two varieties responded positively to the combined stress, with ZD accumulating more sugars, amino acids, and jasmonic acid. The gene expression and flavonoid content of the flavonoid biosynthesis pathway were significantly different between the two varieties. Weighted gene co‐expression network analysis and co‐expression network analysis based on RNA‐Seq data suggested that the MsMYB12 gene may respond to combined stress by regulating the flavonoid biosynthesis pathway. MsMYB12 can directly bind to the promoter of MsFLS13 and promote its expression. Moreover, MsFLS13 overexpression can enhance flavonol accumulation and antioxidant capacity, which can improve combined stress tolerance. These findings provide new insights into improving alfalfa resistance to combined cold and saline‐alkali stress, showing that flavonoids are essential for plant resistance to combined stresses, and provide theoretical guidance for future breeding programs.

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Metabolomics and transcriptomics analysis revealed the response mechanism of alfalfa to combined cold and saline‐alkali stress

Liu,

Si,

Zhang

et al. 2024

The Plant Journal

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A Metabolomic Analysis of Tomato Fruits in Response to Salt Stress

Sun,

Ma,

Yang

et al. 2024

Horticulturae

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Salt stress affects all stages of tomato growth and development, reducing tomato yield, but moderate salt stress improves tomato quality. To gain a deeper understanding of the effect of salt stress on tomato fruits, a widely targeted metabolomic method based on ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) was used to analyze tomato fruits under three different soil salt contents (CK: 0.05 g·kg−1; MS: 3 g·kg−1; and HS: 6 g·kg−1). A total of 847 metabolites were detected in tomato fruit under salt stress, including six primary metabolites such as lipids, amino acids and their derivatives, sugars, nucleotides and their derivatives, and organic acids, and nine secondary metabolites such as phenolic acids, alkaloids, and flavonoids. There were 54 different metabolites in the three treatments, mainly flavonols and phenolamines. Dopamine, galactotol, and mannitol were not detected in the control group, but their contents were higher in the salt treatment. KEGG analysis showed that the differential metabolites were mainly concentrated in flavonoid biosynthesis, betaine metabolism, sulfur metabolism, and galactose metabolism. This study provides a theoretical basis for the regulation of tomato quality through salt stress.

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Integrated Analysis of Transcriptome and Metabolome Provides Insights into Flavonoid Biosynthesis of Blueberry Leaves in Response to Drought Stress

Feng,

Bai,

Zhou

et al. 2024

IJMS

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Blueberries (Vaccinium spp.) are extremely sensitive to drought stress. Flavonoids are crucial secondary metabolites that possess the ability to withstand drought stress. Therefore, improving the drought resistance of blueberries by increasing the flavonoid content is crucial for the development of the blueberry industry. To explore the underlying molecular mechanism of blueberry in adaptation to drought stress, we performed an integrated analysis of the metabolome and transcriptome of blueberry leaves under drought stress. We found that the most enriched drought-responsive genes are mainly involved in flavonoid biosynthesis and plant hormone signal transduction pathways based on transcriptome data and the main drought-responsive metabolites come from the flavonoid class based on metabolome data. The UDP-glucose flavonoid 3-O-glucosyl transferase (UFGT), flavonol synthase (FLS), and anthocyanidin reductase (ANR-2) genes may be the key genes for the accumulation of anthocyanins, flavonols, and flavans in response to drought stress in blueberry leaves, respectively. Delphinidin 3-glucoside and delphinidin-3-O-glucoside chloride may be the most important drought-responsive flavonoid metabolites. VcMYB1, VcMYBPA1, MYBPA1.2, and MYBPA2.1 might be responsible for drought-induced flavonoid biosynthesis and VcMYB14, MYB14, MYB102, and MYB108 may be responsible for blueberry leaf drought tolerance. ABA responsive elements binding factor (ABF) genes, MYB genes, bHLH genes, and flavonoid biosynthetic genes might form a regulatory network to regulate drought-induced accumulation of flavonoid metabolites in blueberry leaves. Our study provides a useful reference for breeding drought-resistant blueberry varieties.

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Genome-wide identification and characterization of flavonol synthase (FLS) gene family in Brassica vegetables and their roles in response to biotic and abiotic stresses

Cited by 3 publications

References 61 publications

Metabolomics and transcriptomics analysis revealed the response mechanism of alfalfa to combined cold and saline‐alkali stress

Metabolomics and transcriptomics analysis revealed the response mechanism of alfalfa to combined cold and saline‐alkali stress

A Metabolomic Analysis of Tomato Fruits in Response to Salt Stress

Integrated Analysis of Transcriptome and Metabolome Provides Insights into Flavonoid Biosynthesis of Blueberry Leaves in Response to Drought Stress

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