Genome-wide identification and characterization of Toll-like receptor genes in black rockfish (Sebastes schlegelii) and their response mechanisms following poly (I:C) injection

Han, Fei; Zhang, Yuan; Xu, Anle; Wang, Xiaoyan; He, Yan; Song, Na; Gao, Tianxiang

doi:10.1016/j.cbpc.2022.109277

Cited by 4 publications

(6 citation statements)

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“…SmaTLR7 showed the highest expression level in the kidneys. Similar expression patterns of TLR7 have been reported in black rockfish and mandarin fish [ 37 , 63 ]. SmaTLR13 was mainly expressed in the gill.…”

Section: Discussionsupporting

confidence: 80%

“…SmaTLR5S only had the LRR domain; this was the main difference between TLR5M and TLR5S [ 36 ]. TLR5S without the TIR domain or transmembrane region was also found in black rockfish [ 37 ], Atlantic salmon ( Salmon salar ) [ 38 ], Japanese flounder ( Paralichthys olivaceus ) [ 10 ], etc. Teleost could encode most of the TLR homologous proteins found in mammals, birds, and amphibians (TLR1~5, TLR7~9, and TLR12~13), as well as more than ten kinds of fish-specific TLRs (TLR14, TLR18~28) [ 8 , 9 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, Nile tilapia TLR13a and TLR13b were significantly highly expressed in the spleen, and TLR21 in the lined seahorse ( Hippocampus erectus ) was mostly detected in its kidney and gill [ 64 , 65 ]. It was observed that TLR22-1 and TLR22-2 of common carp, and TLR22 of black rockfish were highly expressed in the spleen [ 32 , 37 ]. In Japanese flounder, TLR22 showed high expression levels in the spleen, liver, pituitary, and gill [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the spleen and head kidney of marbled rockfish, the expression of TLR3 was significantly upregulated after poly(I:C) injection. TLR3 was also upregulated under poly(I:C) stimulation in the spleen of large yellow croaker, zebrafish, and mandarin fish [ 63 , 67 , 68 ], and in the head kidney of Japanese meagre and black rockfish [ 37 , 58 ]. In the virus infection experiment of rainbow trout and rare minnow, TLR3 expression was significantly upregulated in immune tissues [ 69 , 70 ].…”

Section: Discussionmentioning

confidence: 99%

“…TLR7 has been reported to recognize viral RNA and activate the antiviral immune mechanism in fish [ 62 , 71 ]. In the studies of large yellow croaker, Japanese meagre and black rockfish, TLR7 showed a similar regulation tendency with SmaTLR7 in the head kidney or spleen after poly(I:C) infection [ 37 , 58 , 73 ], implying that the virus analogues could also cause the immune response of fish TLR7.…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Identification, Characterization and Expression Analysis of Toll-like Receptors in Marbled Rockfish (Sebastiscus marmoratus)

Zhang

Wang

Han

et al. 2022

IJMS

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Toll-like receptor (TLR) is a cluster of type I transmembrane proteins that plays a role in innate immunity. Based on the marbled rockfish (Sebastiscus marmoratus) genome database, this study used bioinformatics methods to identify and analyze its TLR gene family members. The results showed that there were 11 TLR gene family members in Sebastiscus marmoratus (SmaTLR), which could be divided into five different subfamilies. The number of amino acids encoded by the Smatlr genes ranged from 637 to 1206. The physicochemical properties of the encoded proteins of different members were also computed. The results of protein structure prediction, phylogenetic relation, and motif analysis showed that the structure and function of the SmaTLRs were relatively conserved. Quantitative Real-Time PCR (qRT-PCR) analysis revealed the expression patterns of SmaTLRs in the gill, liver, spleen, head kidney, kidney, and intestine. SmaTLRs were widely detected in the tested tissues, and they tended to be expressed higher in immune-related tissues. After polyriboinosinic polyribocytidylic acid (poly(I:C)) challenge, SmaTLR14, SmaTLR3, SmaTLR5S, SmaTLR7, and SmaTLR22 were significantly upregulated in the spleen or liver. The results of this study will help to understand the status of TLR gene family members of marbled rockfish and provide a basis for further study of the functional analysis of this gene family.

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Section: Discussionsupporting

confidence: 80%