Genome-Wide Identification and Characterization of Warming-Related Genes in Brassica rapa ssp. pekinensis

Abstract

For sustainable crop cultivation in the face of global warming, it is important to unravel the genetic mechanisms underlying plant adaptation to a warming climate and apply this information to breeding. Thermomorphogenesis and ambient temperature signaling pathways have been well studied in model plants, but little information is available for vegetable crops. Here, we investigated genes responsive to warming conditions from two Brassica rapa inbred lines with different geographic origins: subtropical (Kenshin) and temperate (Chiifu). Genes in Gene Ontology categories “response to heat”, “heat acclimation”, “response to light intensity”, “response to oxidative stress”, and “response to temperature stimulus” were upregulated under warming treatment in both lines, but genes involved in “response to auxin stimulus” were upregulated only in Kenshin under both warming and minor-warming conditions. We identified 16 putative high temperature (HT) adaptation-related genes, including 10 heat-shock response genes, 2 transcription factor genes, 1 splicing factor gene, and 3 others. BrPIF4, BrROF2, and BrMPSR1 are candidate genes that might function in HT adaptation. Auxin response, alternative splicing of BrHSFA2, and heat shock memory appear to be indispensable for HT adaptation in B. rapa. These results lay the foundation for molecular breeding and marker development to improve warming tolerance in B. rapa.

High ambient temperature leads to reduced FT expression and delayed flowering in Brassica rapa via a mechanism associated with H2A.Z dynamics

“…However, we found that the B. rapa FT.a locus showed increased H2A.Z levels at high ambient temperature compared with 21°C (Figures c and S5). To determine if this effect was specific for FT.a or may reflect a more general response to the heat stimulus, we studied some genes previously shown to be misexpressed by heat stress in B. rapa (Dong et al ., ; Song et al ., ). We found that BraA.HSP23 , BraA.HSP18 and BraA.bHLH expression was clearly upregulated, whereas BraA.SOB5 and BraA.GRSX expression was downregulated at 28°C compared with 21°C in B. rapa leaves (Figure a,b).…”

Antifungal Peptide P852 Effectively Controls Fusarium oxysporum, a Wilt-Causing Fungus, by Affecting the Glucose Metabolism and Amino Acid Metabolism as well as Damaging Mitochondrial Function

“…31 After recombinant expression of the Bacillus subtilis antifungal gene sequence Mt330, the Fusarium graminearum mycelial growth rate and biomass were reduced, while the intracellular BCAA pathway was significantly affected. 32 The addition of L-Ile and L-Pro significantly promotes F. oxysporum spore formation when the TORC1-Tap42 signaling pathway is inhibited. 33 Fu et al mentioned that arginine and proline metabolisms are closely related to the pathogenicity of fungi.…”