Genome-wide identification and classification of theHsfandsHspgene families inPrunus mume, and transcriptional analysis under heat stress

Abstract

The transcriptional activation of heat shock proteins (Hsps) by heat shock transcription factors (Hsfs) is presumed to have a pivotal role in plant heat stress (HS) response. Prunus mume is an ornamental woody plant with distinctive features, including rich varieties and colors. In this study, 18 Hsfs and 24 small Hsps (sHsps) were identified in P. mume. Their chromosomal locations, protein domains, conserved motifs, phylogenetic relationships, and exon–intron structures were analyzed and compared with Arabidopsis thaliana Hsfs or sHsps. A total of 18 PmHsf members were classified into three major classes, A, B, and C. A total of 24 PmsHsps were grouped into eight subfamilies (CI to CIII, P, endoplasmic reticulum, M, and CI- or P-related). Quantitative reverse transcription PCR analysis revealed that members of the A2, A7, and A9 groups became the prominent Hsfs after heat shock, suggesting their involvement in a key regulatory role of heat tolerance. Most of the PmsHsp genes were up-regulated upon exposure to HS. Overall, our data contribute to an improved understanding of the complexity of the P. mume Hsf and sHsp gene families, and provide a basis for directing future systematic studies investigating the roles of the Hsf and sHsp gene families.

Plant small heat shock proteins – evolutionary and functional diversity

“…1a), there are six classes encoding proteins that are targeted to each membrane‐bound organelle in the cell: chloroplasts (CP), mitochondria (MTII), the endoplasmic reticulum (ER), peroxisomes (PX) and the nucleus (CIII), as well as proteins reported to be dual‐targeted to both mitochondria and chloroplasts (MTI/CP) (Van Aken et al , 2009; Basha et al , 2012). The conserved nature of these classes is supported by ever‐increasing analysis of sHSPs in annotated plant genomes and transcriptomes (Table 2) (Tao et al , 2015; Ma et al , 2016; Paul et al , 2016; Yu et al , 2016; Muthusamy et al , 2017; X. Wang et al , 2017; Yan et al , 2017; Yang et al , 2017; He et al , 2018; J. Li et al , 2018; Liu et al , 2018; Li & Liu, 2019; Ma et al , 2019; Nagaraju et al , 2019; Wan et al , 2019). The intracellular localization of the organelle‐targeted proteins has been confirmed by imaging and/or biochemical studies in at least one plant species.…”

Genome-Wide Identification and Characterization of Heat-Shock Transcription Factors in Rubber Tree

“…Plant Hsfs are terminal components of a signal-transduction chain mediating the expression of abiotic-stress-responsive genes [1]. The number of Hsf members is 18 in plums [15], 21 in Arabidopsis [5], 25 in rice [6], 25 in apples [8], and 32 in cassavas [25]; these plants are diplontic. The number of Hsf members increases in allopolyploids-there are 43 in triploid banana plants [13], 59 in tetraploid soybean plants [9], and 56 in sextaploid wheat plants [10].…”

“…By activating their target stress-related genes, Hsfs play an important role in regulating plant adaption to various abiotic stresses [1]. Genome-wide identification of Hsfs has been performed in plants such as Arabidopsis [5], rice [6,7], apple [8], soybean [9], wheat [10], Chinese cabbage [11], strawberry [12], banana [13], rape [14], and plum [15]. These works provide a basis for integrating Hsf function into the regulation of plant growth and development by adaption to avoidable abiotic stresses.…”

Identification, expression, and functional analysis of Hsf and Hsp20 gene families in Brachypodium distachyon under heat stress

“…Numerous Hsp and Hsf genes have been reported in higher plants such as Prunus mume (Wan et al, 2019) and Populus trichocarpa (Zhang et al, 2015). It is well-known that tissuespecific expression patterns may enable Hsp and Hsf proteins to have distinct functions in different signaling pathways, although Hsp and Hsf proteins usually show low abundance under normal conditions.…”