Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus

Abstract

The C2H2-type zinc finger proteins (C2H2-ZFPs) regulate various developmental processes and abiotic stress responses in eukaryotes. Yet, a comprehensive analysis of these transcription factors which could be used to find candidate genes related to the control the development and abiotic stress tolerance has not been performed in Pleurotus ostreatus. To fill this knowledge gap, 18 C2H2-ZFs were identified in the P. ostreatus genome. Phylogenetic analysis indicated that these proteins have dissimilar amino acid sequences. In addition, these proteins had variable protein characteristics, gene intron-exon structures, and motif compositions. The expression patterns of PoC2H2-ZFs in mycelia, primordia, and young and mature fruiting bodies were investigated using qRT-PCR. The expression of some PoC2H2-ZFs is regulated by auxin and cytokinin. Moreover, members of PoC2H2-ZFs expression levels are changed dramatically under heat and cold stress, suggesting that these genes may participate in abiotic stress responses. These findings could be used to study the role of P. ostreatus-derived C2H2-ZFs in development and stress tolerance.

Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Its Response to Cold and Drought Stress in Sorghum [Sorghum bicolor (L.) Moench]

“…Previous reports indicate that they play an important role in cucumber, Arabidopsis , wheat, and tomato [ 39 , 40 , 41 , 42 ]. Therefore, many researchers have tried to perform genome-wide analysis on C2H2-ZFP s in various species, such as tomato, wheat, grape, and oyster mushroom [ 43 , 44 , 45 , 46 ], but little is known about sorghum C2H2-ZFP proteins. In this study, we carried out a genome-wide study of the S. bicolor C2H2-ZFP family and identified a total of 145 SbC2H2-ZFP members.…”

“…The expression of a gene is often used to predict its function. Previous findings have shown that the expression of C2H2-ZFP genes was affected by tissue differences and various abiotic stresses [ 46 , 53 ]. Our results showed that among the selected SbC2H2-ZFPs, most genes were expressed in roots higher than those in leaves or stems, but almost all SbC2H2-ZFP members were expressed in roots, stems, and leaves.…”

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

“…No such changes were observed for most other enzymes [ 29 ]. The expression of genes coding for zinc finger proteins in P. ostreatus was regulated by developmental processes, phytoeffectors and abiotic stress [ 30 ], whereas, in line with a genetic study [ 13 ], the spectrum of lectins changed from mycelium to primordium to mature fruiting body [ 31 ]. A lentinan degrading β -1,3-glucanase of L. edodes occurred after the harvest of fruiting bodies but was not detectable in mycelia and young fruiting bodies [ 32 ].…”

“…They thrive on most of the substrates shown in Table 1 by quickly inducing large enzymatic networks (own experiments, data not shown). As a result, the composition of the fruiting bodies depends on the growth substrate [ 33 , 55 , 57 , 60 , 67 , 72 , 74 ], the strain chosen [ 33 , 60 , 74 , 75 , 76 , 77 , 78 , 80 ], its developmental stage [ 29 , 30 , 31 , 32 , 38 ], and the associated adverse or beneficial microbial community [ 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ] and is thus variable like no other food. The same applies to the mass production of fungal mycelia.…”

Genome-Wide Identification and Expression Analysis of the Zinc Finger Protein Gene Subfamilies under Drought Stress in Triticum aestivum

“…Due to the extensive interest in their positions in the physiological regulation of resistance, some ZFP families have been identified and investigated in various plants, such as A. thaliana , Solanum tuberosum , Pleurotus ostreatus, and Cucumis sativus [ 18 , 19 , 20 , 21 , 22 ]. However, this functionally diverse family of proteins has not been studied in wheat ( Triticum aestivum ).…”