Genome-wide identification and expression analysis of the GRAS gene family in response to drought stress in chickpea (Cicer arietinum L.)

Yadav, Sheel; Yadava, Yashwant K.; Kohli, D.; Meena, Shashi; Paul, Vijay; Jain, Pradeep

doi:10.1007/s13205-021-03104-z

Cited by 7 publications

(8 citation statements)

References 48 publications

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“…In this study, 46 DchGRAS genes were obtained through comparison and screening based on GRAS-specific domains (Table 1). The identification results were similar to those of Cicer arietinum (46) (Yadav et al, 2022), Prunus mume (47) (Lu et al, 2015), Dendrobium catenatum (47) (Zeng et al, 2019); more than those of A. thaliana (32) (Tian et al, 2004) and Pinus (32) (Abarca et al, 2014); and less than those of Rosa chinensis (59) (Kumari et al, 2022), O. sativa (63) (Tian et al, 2004), Manihot esculenta (77) (Shan et al, 2020), and Zea mays (86) (Guo et al, 2017). These differences may be due to gene duplication events or the different frequencies of retained copies after duplication events.…”

Section: B C a Figuresupporting

confidence: 81%

Genome-wide identification and expression analysis of the GRAS gene family in Dendrobium chrysotoxum

et al. 2022

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The GRAS gene family encodes transcription factors that participate in plant growth and development phases. They are crucial in regulating light signal transduction, plant hormone (e.g. gibberellin) signaling, meristem growth, root radial development, response to abiotic stress, etc. However, little is known about the features and functions of GRAS genes in Orchidaceae, the largest and most diverse angiosperm lineage. In this study, genome-wide analysis of the GRAS gene family was conducted in Dendrobium chrysotoxum (Epidendroideae, Orchidaceae) to investigate its physicochemical properties, phylogenetic relationships, gene structure, and expression patterns under abiotic stress in orchids. Forty-six DchGRAS genes were identified from the D. chrysotoxum genome and divided into ten subfamilies according to their phylogenetic relationships. Sequence analysis showed that most DchGRAS proteins contained conserved VHIID and SAW domains. Gene structure analysis showed that intronless genes accounted for approximately 70% of the DchGRAS genes, the gene structures of the same subfamily were the same, and the conserved motifs were also similar. The Ka/Ks ratios of 12 pairs of DchGRAS genes were all less than 1, indicating that DchGRAS genes underwent negative selection. The results of cis-acting element analysis showed that the 46 DchGRAS genes contained a large number of hormone-regulated and light-responsive elements as well as environmental stress-related elements. In addition, the real-time reverse transcription quantitative PCR (RT−qPCR) experimental results showed significant differences in the expression levels of 12 genes under high temperature, drought and salt treatment, among which two members of the LISCL subfamily (DchGRAS13 and DchGRAS15) were most sensitive to stress. Taken together, this paper provides insights into the regulatory roles of the GRAS gene family in orchids.

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Section: B C a Figuresupporting

confidence: 81%

Genome-wide identification and expression analysis of the GRAS gene family in Dendrobium chrysotoxum

et al. 2022

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“…Our previous study showed that white lupin has evolved from a whole-genome triplication (WGT) event, resulting in the expansion of genome through tandem and dispersed duplications (Xu et al, 2020). The number of GRAS genes in white lupin is comparable to other species with 46 GRAS genes reported in chickpea (Yadav et al, 2022), 59 in Medicago sativa, 34 in A. thaliana (Liu & Widmer, 2014), 60 in O. sativa (Liu & Widmer, 2014), and 117 in G.…”

Section: Discussionmentioning

confidence: 95%

“…Several studies have shown that various root traits are regulated by a series of transcription factors (TFs). For instance, GRAS TFs are involved in regulating root pattering and elongation (Yadav et al, 2022), root cell elongation, and root system improvement (Zhang et al, 2017). Additionally, GRAS TF are also involved in several abiotic and biotic stress responses.…”

Section: Discussionmentioning

confidence: 99%

“…They play diverse roles in plant growth and development, and tolerance to various stresses (Waseem et al, 2022). For instance, GRAS TFs were reported to be involved in root development, root radial organization (Helariutta et al, 2000; Heo et al, 2011), root patterning (Yadav et al, 2022), meristems formation (Engstrom et al, 2011; Li et al, 2003), and stress responses (Chen et al, 2015; Ma et al, 2010). In Ipomoea trifida , 28 GRAS genes were highly expressed in the roots (Chen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of LaGRAS enhances phosphorus acquisition via increased root growth of phosphorus‐deficient white lupin

Aslam

Fritschi

Zhang

et al. 2023

Physiologia Plantarum

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The GRAS transcription factors play an indispensable role in plant growth and responses to environmental stresses. The GRAS gene family has extensively been explored in various plant species; however, the comprehensive investigation of GRAS genes in white lupin remains insufficient. In this study, bioinformatics analysis of white lupin genome revealed 51 LaGRAS genes distributed into 10 distinct phylogenetic clades. Gene structure analyses revealed that LaGRAS proteins were considerably conserved among the same subfamilies. Notably, 25 segmental duplications and a single tandem duplication showed that segmental duplication was the major driving force for the expansion of GRAS genes in white lupin. Moreover, LaGRAS genes exhibited preferential expression in young cluster root and mature cluster roots and may play key roles in nutrient acquisition, particularly phosphorus (P). To validate this, RT‐qPCR analysis of white lupin plants grown under +P (normal P) and −P (P deficiency) conditions elucidated significant differences in the transcript level of GRAS genes. Among them, LaGRAS38 and LaGRAS39 were identified as potential candidates with induced expression in MCR under −P. Additionally, white lupin transgenic hairy root overexpressing OE‐LaGRAS38 and OE‐LaGRAS39 showed increased root growth, and P concentration in root and leaf compared to those with empty vector control, suggesting their role in P acquisition. We believe this comprehensive analysis of GRAS members in white lupin is a first step in exploring their role in the regulation of root growth, tissue development, and ultimately improving P use efficiency in legume crops under natural environments.

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“…(2018) Chenopodium quinoa (quinoa) Amaranthaceae Eudicot 52 14 Zhu et al. (2021) Cicer arietinum (chickpea) Fabaceae Eudicot 46 9 Yadav et al. (2022) Citrullus lanatus (watermelon) Cucurbitaceae Eudicot 37 10 Lv et al.…”

Section: Gras Gene Family: Expansion and Diversification In The Plantsmentioning

confidence: 99%

Multifaceted roles of GRAS transcription factors in growth and stress responses in plants

Jaiswal¹,

Kakkar²,

Kumari³

et al. 2022

iScience

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Genome-wide identification and expression analysis of the GRAS gene family in response to drought stress in chickpea (Cicer arietinum L.)

Cited by 7 publications

References 48 publications

Genome-wide identification and expression analysis of the GRAS gene family in Dendrobium chrysotoxum

Genome-wide identification and expression analysis of the GRAS gene family in Dendrobium chrysotoxum

Overexpression of LaGRAS enhances phosphorus acquisition via increased root growth of phosphorus‐deficient white lupin

Multifaceted roles of GRAS transcription factors in growth and stress responses in plants

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