Genome-Wide Identification and Expression Analysis of TPS Gene Family in Liriodendron chinense

Abstract

Terpenoids play a key role in plant growth and development, supporting resistance regulation and terpene synthase (TPS), which is the last link in the synthesis process of terpenoids. Liriodendron chinense, commonly called the Chinese tulip tree, is a rare and endangered tree species of the family Magnoliaceae. However, the genome-wide identification of the TPS gene family and its transcriptional responses to development and abiotic stress are still unclear. In the present study, we identified a total of 58 TPS genes throughout the L. chinense genome. A phylogenetic tree analysis showed that they were clustered into five subfamilies and unevenly distributed across six chromosomes. A cis-acting element analysis indicated that LcTPSs were assumed to be highly responsive to stress hormones, such as methyl jasmonate (MeJA) and abscisic acid (ABA). Consistent with this, transcriptome data showed that most LcTPS genes responded to abiotic stress, such as cold, drought, and hot stress, at the transcriptional level. Further analysis showed that LcTPS genes were expressed in a tissue-dependent manner, especially in buds, leaves, and bark. Quantitative reverse transcription PCR (qRT-PCR) analysis confirmed that LcTPS expression was significantly higher in mature leaves compared to young leaves. These results provide a reference for understanding the function and role of the TPS family, laying a foundation for further study of the regulation of TPS in terpenoid biosynthesis in L. chinense.

The Identification and Expression Analysis of the Liriodendron chinense F-Box Gene Family

“…The 2019 L. chinense genome [ 29 ] has provided valuable resources for genome-wide analyses of gene families that shape the growth, development, and response to environmental stressors of this tree species. Several gene families have been studied, including CBF [ 30 ], WRKY [ 31 ], MYB [ 32 ], TPS [ 33 ], PIN [ 34 ], etc. Here, we conducted a genome-wide gene family analysis of F-box genes in L. chinense , exploring various aspects such as conserved domains, gene structure, phylogenetic tree, chromosome locations, gene replication, and collinearity.…”

Combined Analysis of Volatile Compounds and Extraction of Floral Fragrance Genes in Two Dendrobium Species

“…TPSs are highly differentiated gene families that have been found in Arabidopsis thaliana, Solanum lycopersicum, and Vitis vinifera [24]. In recent years, genome-wide analyses of TPSs have identified them in plants such as Gossypium hirsutum [25], Brachypodium distachyon [26], and Liriodendron chinense [27]. Bioinformatics techniques were used to compare plant TPS family members, which can be divided into 8 subfamilies: TPSa (sesquiterpenes), TPSb (cyclic monoterpenes and hemiterpenes), TPSc (copalyl diphospate synthases), TPSd (gymnosperm-specific), TPSe (ent-kaurene synthases), TPSf (other diterpene synthases), TPSg (acyclic monoterpenes), and TPSh (lycopod-specific) [28].…”