Genome-wide identification and expression analysis of CaM/CML genes in Brassica napus under abiotic stress

He, Xin; Liu, Wei; Li, Wenqian; Liu, Yan; Wang, Weiping; Xie, Ping; Kang, Yu; Liao, Li; Qian, Lunwen; Liu, Zhongsong; Guan, Chunyun; Guan, Mei; Hua, Wei

doi:10.1016/j.jplph.2020.153251

Cited by 26 publications

(21 citation statements)

References 61 publications

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“…AtCML44 is strongly induced by heat [6]. Two BnaCML44 genes (BnaA06g15280D and BnaC05g16860D) are enhanced by heat [7]. In this study, HT (42 • C) significantly induced SlCML39 expression, reaching the maximum in 3 h (Figure 5).…”

Section: Slcml39 Expression Is Induced By Ht As Well As Other Stressessupporting

confidence: 46%

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Characteristics of SlCML39, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of Arabidopsis thaliana during Germination and Seedling Growth

Ding

Qian

Fang

et al. 2021

IJMS

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Calmodulin-like (CML) proteins are primary calcium sensors and function in plant growth and response to stress stimuli. However, so far, the function of plant CML proteins, including tomato, is still unclear. Previously, it was found that a tomato (Solanum lycopersicum) CML, here named SlCML39, was significantly induced by high temperature (HT) at transcription level, but its biological function is scarce. In this study, the characteristics of SlCML39 and its role in HT tolerance were studied. SlCML39 encodes a protein of 201 amino acids containing four EF hand motifs. Many cis-acting elements related to plant stress and hormone response appear in the promoter regions of SlCML39. SlCML39 is mainly expressed in the root, stem, and leaf and can be regulated by HT, cold, drought, and salt stresses as well as ABA and H2O2. Furthermore, heterologous overexpression of SlCML39 reduces HT tolerance in Arabidopsis thaliana at the germination and seedling growth stages. To better understand the molecular mechanism of SlCML39, the downstream gene network regulated by SlCML39 under HT was analyzed by RNA-Seq. Interestingly, we found that many genes involved in stress responses as well as ABA signal pathway are down-regulated in the transgenic seedlings under HT stress, such as KIN1, RD29B, RD26, and MAP3K18. Collectively, these data indicate that SlCML39 acts as an important negative regulator in response to HT stress, which might be mediated by the ABA signal pathway.

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Section: Slcml39 Expression Is Induced By Ht As Well As Other Stressessupporting

confidence: 46%

“…CMLs have one to six EF hands [4]. According to the existing genome database, many CMLs are identified in plants [5], such as 50 CMLs in Arabidopsis thaliana [6], 46 in Medicago truncatula [2], and 168 in Brassica napus [7]. Despite so many CML genes, the functions of most plant CMLs are not well studied [8].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of SlCML39, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of Arabidopsis thaliana during Germination and Seedling Growth

Ding

Qian

Fang

et al. 2021

IJMS

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“…Ca 2+ signaling regulates numerous abiotic stress reactions [ 16 ]. Calmodulin (CaM) and calmodulin-like (CML) proteins [ 17 ] are conventional Ca 2+ -binding proteins, which are generally comprised of one to six EF-hand motifs [ 18 ]. It has been reported that CMLs initiate cellular responses by binding to relevant transcription factors (TFs) (e.g., AP2/ERFs and bHLHs) and transmitting calcium signaling downstream [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics Reveals the ERF2-bHLH2-CML5 Module Responses to H2S and ROS in Postharvest Calcium Deficiency Apples

Sun

Zhang

et al. 2021

IJMS

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Calcium deficiency usually causes accelerated quality deterioration in postharvest fruit, whereas the underlining mechanism is still unclear. Here, we report that calcium deficiency induced the development of bitter pit on the surface of apple peels compared with the healthy appearance in control apples during postharvest storage. Physiological analysis indicates that calcium-deficient peels contained higher levels of superoxide anion (O2•−), malondialdehyde (MDA), total phenol, flavonoid contents and polyphenol oxidase (PPO) activity, and reduced calcium, H2S production, anthocyanin, soluble protein content, and peroxidase (POD) activity compared with those in calcium-sufficient peels. The principal component analysis (PCA) results show that calcium content, ROS, and H2S production were the main factors between calcium-deficient and calcium-sufficient apple peels. Transcriptome data indicated that four calmodulin-like proteins (CMLs), seven AP2/ERFs, and three bHLHs transcripts were significantly differentially expressed in calcium-deficient apple peels. RT-qPCR and correlation analyses further revealed that CML5 expression was significantly positively correlated with the expression of ERF2/17, bHLH2, and H2S production related genes. In addition, transcriptional co-activation of CML5 by ERF2 and bHLH2 was demonstrated by apple transient expression assays and dual-luciferase reporter system experiments. Therefore, these findings provide a basis for studying the molecular mechanism of postharvest quality decline in calcium-deficient apples and the potential interaction between Ca2+ and endogenous H2S.

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“…In grapevine, the expression of CML21 is positively regulated by heat, cold, high salinity and drought conditions (Aleynova et al 2020). Abiotic stress treated transcriptome data of Brassica napus revealed the alter expression of BnCaMs and BnCMLs genes (He et al 2020). Overall, these examples of CaM and CMLs (and their interacting partner CAMTA) suggest their crucial role in Ca 2+ signaling mediated modulation of plant growth and adaptation to abiotic stress.…”

Section: Introductionmentioning

confidence: 93%

Ectopic expression of finger millet calmodulin confers drought and salinity tolerance in Arabidopsis thaliana

et al. 2021

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Drought and salinity are major environmental stresses which affect crop productivity and therefore are major hindrance in feeding growing population world-wide. Calcium (Ca 2+ ) signalling plays a crucial role during the plant's response to these stress stimuli. Calmodulin (CaM), a crucial Ca 2+ sensor, is involved in transducing the signal downstream in various physiological, developmental and stress responses by modulating a plethora of target proteins. The role of CaM has been well established in the model plant Arabidopsis thaliana for regulating various developmental processes, stress signalling and ion transport. In the current study, we investigate the CaM of Eleusine coracana (common name nger millet, known especially for its drought tolerance and superior Ca 2+ content). In-silico analysis showed that Eleucine calmodulin (EcCaM) has greater similarity to rice CaM as compared to Arabidopsis CaM due to the presence of highly conserved four EF-hand domains. To decipher the in planta function of EcCaM, we have adopted the gain-of-function approach by generating the 35S::EcCaM over-expression transgenic in Arabidopsis. Overexpression of EcCaM in Arabidopsis makes the plant tolerant to polyethylene glycol (PEG) induced drought and salt stress (NaCl) as demonstrated by post-germination based phenotypic assay, ion leakage, MDA and proline estimation, ROS detection under stressed and normal conditions. Moreover, EcCaM-overexpression leads to hypersensitivity towards exogenously applied ABA at the seed germination stage. These ndings reveal that EcCaM mediates tolerance to drought and salinity stress. Also, our results indicate that EcCaM is involved in modulating ABA signalling. Summarizing our results, we report for the rst time that EcCaM is involved in modulating plants response to stress and this information can be used for the generation of future-ready crops that can tolerate a wide range of abiotic stresses.

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Genome-wide identification and expression analysis of CaM/CML genes in Brassica napus under abiotic stress

Cited by 26 publications

References 61 publications

Characteristics of SlCML39, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of Arabidopsis thaliana during Germination and Seedling Growth

Characteristics of SlCML39, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of Arabidopsis thaliana during Germination and Seedling Growth

Transcriptomics Reveals the ERF2-bHLH2-CML5 Module Responses to H2S and ROS in Postharvest Calcium Deficiency Apples

Ectopic expression of finger millet calmodulin confers drought and salinity tolerance in Arabidopsis thaliana

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