Genome-Wide Identification and Expression Analysis of the Dof Transcription Factor Gene Family in Gossypium hirsutum L.

Abstract

Gossypium hirsutum L. is a worldwide economical crop; however, premature leaf senescence reduces its production and quality which is regulated by stresses, hormones, and genes. DNA binding with the one zinc finger (Dof) transcription factors (TFs) participate widely in plant development and responses to biotic and abiotic stresses, but there have been few reports of these TFs in cotton. Here, we perform a genome-wide study of G. Hirsutum L. Dof (GhDof) genes and analyze their phylogeny, duplication, and expression. In total, 114 GhDof genes have been identified and classified into nine subgroups (A, B1, B2.2, B2.1, C1, C2.1, C2.2, D1, and D2) based on phylogenetic analysis. An MCScanX analysis showed that the GhDof genes expanded due to segmental duplications. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that GhDofD9.6 was not only differentially expressed between CCRI10 (with premature senescence) and Liao4086 (without premature senescence) but also responded to salinity stress; GhDofA5.7, GhDofA7.4, GhDofA8.2, GhDof11.1, GhDofD7.2, and GhDofD11.3 signfificantly responded to cold (4 °C) stress. This work lays the foundation for further analysis of the function of GhDof genes in G. hirsutum, which will be helpful for improving the production and quality of cotton.

Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon

“…In this study, more than half of the ClDof genes (20 out of 36) exhibited segmental duplications, while no tandem duplication was identified in the watermelon chromosomes, suggesting that segmental duplication has been predominant in the expansion of the Dof genes in watermelon. Similar results have also been reported in other plants such as cotton (Li et al, 2018).…”

Genome-wide analysis and comparison of the DNA-binding one zinc finger gene family in diploid and tetraploid cotton (Gossypium)

“…The salinity and cold treatments induced G. hirsutum Dof transcript accumulation, and the overexpressed of Dof showed significantly higher salt and cold tolerance over the wild-type plants [21]. Moreover, genome-wide study shown that there were 114 Dof genes in G. hirsutum, the phylogeny, duplication, and chromosomal locations of G. hirsutum Dof gene family in previous studies are similar to ours [22]. In this study, we performed a genome-wide analysis and comparison of the Dof gene family within two tetraploid cotton species and two diploid cotton species.…”

“…Because G. hirsutum is the main source of textile fiber, the study of cotton Dof gene family was focused on G. hirsutum in previous studies [21,22]. Studies have shown that G. hirsutum Dof gene family constitutively expressed in leaves, roots and stems, accumulated highest in leaves.…”

“…The Dof gene family has been extensively studied in many plant species, such as Arabidopsis thaliana, Oryza sativa [18], Jatropha curcas [19] and Setaria italic [20]. Furthermore, the Dof gene family of Gossypium hirsutum was also studied [21,22]. Because of the importance of Dof gene in various physiological processes, it would be necessary to perform a genome-wide identification and comparative analysis of Dof family in different cotton species.…”

Genome-Wide Analysis of Dof Genes and Their Response to Abiotic Stress in Rose (Rosa chinensis)

“…The results indicate that segmental duplication has played a predominant role in the evolution of the RchDof genes. In other plants, the majority of Dof genes were also found to derive from segmental duplication events, such as in apple ( Zhang et al, 2018 ), cassava ( Zou et al, 2019 ), tomato ( Cai et al, 2013 ), and cotton ( Li et al, 2018 ). In addition, the majority of the segmentally duplicated genes belonged to the same RchDof subgroup, with the exception of two clusters ( RchDof5 / RchDof17 and RchDof7 / RchDof24 ) ( Table 2 ).…”