Genome-wide identification and expression analysis of the HVA22 gene family in cotton and functional analysis of GhHVA22E1D in drought and salt tolerance

Abstract

The HVA22 family of genes, induced by abscisic acid and stress, encodes a class of stress response proteins with a conserved TB2/DP1/HVA22 domain that are unique among eukaryotes. Previous studies have shown that HVA22s play an important role in plant responses to abiotic stresses. In the present study, 34, 32, 16, and 17 HVA22s were identified in G. barbadense, G. hirsutum, G. arboreum, and G. raimondii, respectively. These HVA22 genes were classified into nine subgroups, randomly distributed on the chromosomes. Synteny analysis showed that the amplification of the HVA22s were mainly due to segmental duplication or whole genome replication (WGD). Most HVA22s promoter sequences contain a large number of drought response elements (MYB), defense and stress response elements (TC-rich repeats), and hormone response elements (ABRE, ERE, SARE, etc.), suggesting that HVA22s may respond to adversity stresses. Expression profiling demonstrated that most GhHVA22s showed a constitutive expression pattern in G. hirsutum and could respond to abiotic stresses such as salt, drought, and low temperature. Overexpression of GhHVA22E1D (GH_D07G0564) in Arabidopsis thaliana enhances salt and drought tolerance in Arabidopsis. Virus-induced gene silencing of GhHVA22E1D reduced salt and drought tolerance in cotton. This indicates that GhHVA22E1D plays an active role in the plant response to salt stress and drought stress. GhHVA22E1D may act in plant response to adversity by altering the antioxidant capacity of plants. This study provides valuable information for the functional genomic study of the HVA22 gene family in cotton. It also provides a reference for further elucidation of the functional studies of HVA22 in plant resistance to abiotic stress response.

Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Cold Shock Protein (CSP) Gene Family in Cotton Hypothermia Stress

“…In comparison, six genes were identified in the D genome of G. hirsutum. Tetraploid cotton is an allotetraploid formed by hybridizing diploids with At and Dt genomes [ 32 ].The number of CSP genes in tetraploid cotton ( G. hirsutum , G. barbadense ) was nearly twice that of diploid cotton ( G. arboreum , G. raimondii ), indicating that the CSP family is conserved throughout evolution. These results indicate that the number of CSPs varies slightly across different cotton species, potentially due to the loss or duplication of genes over the course of cotton evolution.…”

“…Contemporary studies have indicated that CSP activity in plants is influenced by various abiotic stress factors, and the examination of gene expression patterns is often employed to characterize gene function [ 32 ]. Via analysis of expression patterns before and after stress, it was determined that the expression of four genes ( GhCSP.A1 , GhCSP.A2 , GhCSP.A3 , and GhCSP.A7 ) was upregulated under low temperatures.…”

Diurnal.plant.tools in 2024: expanding toMarchantia polymorphaand four angiosperms

“…Most unassigned genes (20) had some form of annotation that could be inferred from the gene family. Of which, orthologs of Mp1g15330.1 (HVA22-like, Zhang et al ., 2023), Mp5g01770.1 (temperature-induced lipocalin, Abo-Ogiala et al ., 2014) and Mp5g15480.1 (interferon-related developmental regulator family protein, Park et al ., 2009) have been implicated in stress response.…”

AtHVA22a, a plant‐specific homologue of Reep/DP1/Yop1 family proteins is involved in turnip mosaic virus propagation

“…In barley vegetative tissues, the expression of HVA22 was also shown to be induced by ABA, cold and drought. Since then, diverse abiotic stresses such as temperature, salinity and drought have been shown to differentially regulate the expression of HVA22 homologues in various plant species (Courtney et al., 2016 ; Gomes Ferreira et al., 2019 ; Lyu et al., 2021 ; Wai et al., 2022 ; Zhang, Wen, et al., 2023 ; Zhang, Yuan, et al., 2023 ). However, only a limited number of studies describe the function of those HVA22 genes in plants.…”

“…In Arabidopsis , reticulon proteins (called RTNLB) have also been described (Nziengui et al., 2007 ). The overexpression of a number of RTNLBs in tobacco leaf epidermal cells is sufficient to constrict the ER lumen and convert ER sheets into tubules (Sparkes et al., 2010 ; Tolley et al., 2008 ; Zhang, Wen, et al., 2023 ; Zhang, Yuan, et al., 2023 ), suggesting a conserved role for these proteins in eukaryotes. The membrane curvature is induced by two long hydrophobic regions displaying a wedge‐like topology (Wang et al., 2021 ).…”