Genome-Wide Identification and Expression Analysis of JAZ Family Involved in Hormone and Abiotic Stress in Sweet Potato and Its Two Diploid Relatives

Abstract

Jasmonate ZIM-domain (JAZ) proteins are key repressors of a jasmonic acid signaling pathway. They play essential roles in the regulation of plant growth and development, as well as environmental stress responses. However, this gene family has not been explored in sweet potato. In this study, we identified 14, 15, and 14 JAZs in cultivated hexaploid sweet potato (Ipomoea batatas, 2n = 6x = 90), and its two diploid relatives Ipomoea trifida (2n = 2x = 30) and Ipomoea triloba (2n = 2x = 30), respectively. These JAZs were divided into five subgroups according to their phylogenetic relationships with Arabidopsis. The protein physiological properties, chromosome localization, phylogenetic relationship, gene structure, promoter cis-elements, protein interaction network, and expression pattern of these 43 JAZs were systematically investigated. The results suggested that there was a differentiation between homologous JAZs, and each JAZ gene played different vital roles in growth and development, hormone crosstalk, and abiotic stress response between sweet potato and its two diploid relatives. Our work provided comprehensive comparison and understanding of the JAZ genes in sweet potato and its two diploid relatives, supplied a theoretical foundation for their functional study, and further facilitated the molecular breeding of sweet potato.

Genome-wide identification and expression analysis of the glutamate receptor gene family in sweet potato and its two diploid relatives

“…Arabidopsis , rice, and other model plants have been investigated for the functions of GLRs , however, until now, there have been no reports on the identification of the GLR gene family members in sweet potato. Because of the complicated genetic background of cultivated sweet potato, research on the sweet potato gene family is limited in the cultivated variety, mostly focusing on its two diploid relatives ( Chen et al., 2019a ; Chen et al., 2019b ; Li et al., 2019 ; Lu et al., 2019 ; Wan et al., 2020 ; Zhu et al., 2020 ; Huang et al., 2021 ; Dai et al., 2022 ; Nie et al., 2023 ). Through this investigation, we conducted an in-depth study of the characteristics of GLR genes and compared their expression patterns in response to different types of biotic and abiotic stresses using genomic sequences for the hexaploid sweet potato and two diploid relatives.…”

A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato

“…The comparative genome-wide study was performed between four Ipomoea species to evaluate valine glutamine motif-containing genes and found 40 differentially expressed genes (DEGs) referring to various abiotic stresses [ 26 ]. The other genome-wide studies in different Ipomoea species were conducted for important TF families such as NAC ( NAM , ATAF , and CUC ) TFs family [ 27 ], hydroxycinnamate-CoA quinate hydroxycinnamoyl transferase gene family [ 28 ], DNA-binding with one finger (Dof) TF family [ 29 ], myeloblastosis (MYB) gene family [ 30 ], phytochrome-interacting factors [ 25 ], domain of unknown function gene family [ 31 ], two-component system genes [ 32 ], xyloglucan endotransglucosylase/hydrolase gene family [ 33 ], expansin gene family [ 34 ], glycine-rich RNA-binding proteins [ 35 ], WRKY TFs family [ 36 ], zinc-finger domain-containing stress-associated proteins [ 37 ], plant-specific GRAS TFs [ 38 ], APETALA2/ethylene responsive factor (AP2/ERF) TFs family [ 38 ], jasmonate-ZIM (JAZ) TF family [ 39 ], and bZIP TF family [ 10 ]. These studies provide new insights for understanding the different TFs families-mediated stress responses and lays the foundation for future functional investigation of sweet potato TFs families.…”

Deciphering the hormone regulatory mechanisms of storage root initiation in sweet potato: challenges and future prospects

“…Jasmonic acid levels show a progressive decline as SR development progresses, with an eventual increase as the SR reaches maturity ( Dong et al 2019 ). Huang et al (2021) investigated the expression of genes encoding the jasmonate ZIM-domain (JAZ) protein family which are involved in JA signalling. IbJAZ3.1 , IbJAZ4 , IbJAZ6.1 , IbJAZ8.2 , and IbJAZ9 were expressed significantly higher in SRs compared to FRs, but these genes were also highly expressed in other plant parts as well.…”

“…IbJAZ3.1 , IbJAZ4 , IbJAZ6.1 , IbJAZ8.2 , and IbJAZ9 were expressed significantly higher in SRs compared to FRs, but these genes were also highly expressed in other plant parts as well. The expression of these genes was repressed by ABA and showed varying responses to GA, IAA, MeJA and SA ( Huang et al 2021 ). Overexpression of the B-box TR, IbBBX24 , led to increased sweet potato yield since IbBBX24 interacts with IbJAZ10 reducing IbMYC2 repression and increasing transcription of genes that are transcriptionally activated by IbMYC2 ( Zhang et al 2020 ).…”