Genome-wide identification and expression analysis of jasmonate ZIM domain gene family in tuber mustard (Brassica juncea var. tumida)

Abstract

Tuber mustard, which is the raw material of Fuling pickle, is a crop with great economic value. However, during growth and development, tuber mustard is frequently attacked by the pathogen Plasmodiophora brassicae and frequently experiences salinity stress. Jasmonic acid (JA) is a hormone related to plant resistance to biotic and abiotic stress. Jasmonate ZIM domain proteins (JAZs) are crucial components of the JA signaling pathway and play important roles in plant responses to biotic and abiotic stress. To date, no information is available about the characteristics of the JAZ family genes in tuber mustard. Here, 38 BjJAZ genes were identified in the whole genome of tuber mustard. The BjJAZ genes are located on 17 of 18 chromosomes in the tuber mustard genome. The gene structures and protein motifs of the BjJAZ genes are conserved between tuber mustard and Arabidopsis. The results of qRT-PCR analysis showed that BjuA030800 was specifically expressed in root, and BjuA007483 was specifically expressed in leaf. In addition, 13 BjJAZ genes were transiently induced by P. brassicae at 12 h, and 7 BjJAZ genes were induced by salt stress from 12 to 24 h. These results provide valuable information for further studies on the role of BjJAZ genes in the regulation of plant growth and development and in the response to biotic and abiotic stress.

New Insights Into Structure and Function of TIFY Genes in Zea mays and Solanum lycopersicum: A Genome-Wide Comprehensive Analysis

“…Several TIFY members were predicted to be located in organelles, such as chloroplasts and mitochondria ( Figure 1 ). These differences in cellular components indicate that the TIFY gene family developed in an extensive regulatory system in plant cells to control various processes ( Bai et al, 2011 ; Cai et al, 2020 ). Previous studies indicated that the members of the TIFY gene family are involved in various mechanisms of plant responses to stress and the regulation of plant growth and development ( Hakata et al, 2012 ; Zhou et al, 2015 ; Sun et al, 2017 ; Liu et al, 2020 ; Wang et al, 2020 ).…”

“…Adverse conditions, such as biotic and abiotic stresses, as limiting factors, affect plant performance. Previous studies revealed that members of the TIFY family, as specific plant TFs, play critical roles in regulating plant responses to adverse environmental conditions ( Ebel et al, 2018 ; Chao et al, 2019 ; Cai et al, 2020 ). For instance, overexpression of apple JAZ2 could significantly improve the tolerance to P. syringae pv.…”

“…Regarding the results, most of the genes with a tendency to express in response to adverse conditions were from the JAZ subfamily, which may reveal the important roles of this subfamily in dealing with stimuli ( Figures 9 , 10 ). Cai et al (2020) recently stated that the JAZ genes of tuber mustard are induced by pathogen Plasmodiophora brassicae and salt stress. JAZ9 in rice can interact with the bHLH062 TF to control salt tolerance via affecting the ion transporter genes ( Wu et al, 2015 ).…”

Genome-wide identification and expression analysis of the JAZ gene family in turnip

“…Previous research identified 36, 38, 36, and 36 JAZ genes in B. rapa L. 40 , B. juncea var. tumida 41 , B. napus L. 42 , and B. oleracea var. capitata 30 , respectively.…”

“…Previous studies showed that no tandem duplication events of JAZ family genes were found in the B. rapa L. and B. juncea var. tumida 40 , 41 , whereas two pairs of tandem duplication genes were identified in Brassica oleracea var. capitata 30 .…”