Genome-Wide Identification and Expression Analysis of ESPs and NSPs Involved in Glucosinolate Hydrolysis and Insect Attack Defense in Chinese Cabbage (Brassica rapa subsp. pekinensis)

Han, Danni; Tan, Jingru; Yue, Zhichen; Peng, Tao; Lei, Juanli; Zang, Yunxiang; Hu, Qizan; Wang, Huasen; Zhang, Shizhong; Li, Biyuan; Zhao, Yong

doi:10.3390/plants12051123

Cited by 2 publications

(1 citation statement)

References 47 publications

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“…Meanwhile, NRT1.5 (BraA05g023200.3C), NRT1.8 (BraA03g049670.3C), NPF3.1(BraA07g030700.3C), and NPF6.4 (BraA05g025490.3C) were highly expressed in L14. Our findings are in accordance with previous studies in B. rapa, Poncirus trifoliata, and Spirodela polyrhiza [39,44,45]. Hence, we assumed these genes could provide valuable allelic variations for the enhancement of nutrient use efficiency (NUE) in Chinese cabbage.…”

Section: Discussionsupporting

confidence: 92%

Genome-Wide Identification of the NRT1 Family Members and Their Expression under Low-Nitrate Conditions in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Zhang,

Ritonga,

Zhang

et al. 2023

Plants

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Nitrate transporters (NRTs) actively take up and transform nitrate (N) to form a large family with many members and distinct functions in plant growth and development. However, few studies have identified them in the context of low nitrate concentrations in Chinese cabbage (Brassica rapa L. ssp. Pekinensis), an important vegetable in China. This study focuses on the identification and analysis of the nitrate transporter 1 (NRT1) gene family as well as various aspects, including its phylogenic distribution, chromosomal position, gene structure, conserved motifs, and duplication pattern. Using bioinformatics methods, we identified and analyzed 84 BrNRT1 genes distributed on ten chromosomes. Furthermore, we conducted an analysis of the expression profile of the NRT1 gene in various tissues of Chinese cabbage exposed to varying nitrate concentrations. A phylogenetic analysis revealed that BrNRT1s members are distributed in six distinct groups. Based on an analysis of gene structure and conserved motifs, it can be inferred that BrNRT1 exhibits a generally conserved structural pattern. The promoters of BrNRT1 were discovered to contain moosefs (MFS) elements, suggesting their potential role in the regulation of NO3− transport across the cell membrane in Chinese cabbage. A transcriptome study and a subsequent RT-qPCR analysis revealed that the expression patterns of some BrNRT1 genes were distinct to specific tissues. This observation implies these genes may contribute to nitrate uptake and transport in various tissues or organs. The results offer fundamental insights into investigating the NRT1 gene family in Chinese cabbage. These results provide basic information for future research on the functional characterization of NRT1 genes in Chinese cabbage and the elucidation of the molecular mechanisms underlying low nitrogen tolerance in Chinese cabbage.

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Section: Discussionsupporting

confidence: 92%

Genome-Wide Identification of the NRT1 Family Members and Their Expression under Low-Nitrate Conditions in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Zhang,

Ritonga,

Zhang

et al. 2023

Plants

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Glucosinolates Mediated Regulation of Enzymatic Activity in Response to Oxidative Stress in Brassica spp.

Gantait,

Masih,

Addesso

et al. 2024

Plants

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Brassica crops are vital as they supply essential minerals, antioxidants, and bioactive substances like anthocyanins, glucosinolates, and carotenoids. However, biotic and abiotic elements that cause oxidative stress through heavy metals and other eco-toxicants pose a risk to Brassica plants. Increased generation of Reactive Oxygen Species (ROS) causes oxidative stress, which damages biomolecules and interferes with plant growth, productivity, and cellular equilibrium. Plants producing Brassica need an intricate enzyme defence mechanism to fend off oxidative stress. All the enzymes that have been addressed are found in mitochondria, peroxisomes, chloroplasts, and other cell components. They are in charge of removing ROS and preserving the cell’s redox balance. Additionally, Brassica plants use secondary metabolites called Glucosinolates (GLs), which have the capacity to regulate enzymatic activity and act as antioxidants. By breaking down compounds like sulforaphane, GLs boost antioxidant enzymes and provide protection against oxidative stress. To develop methods for improving agricultural crop stress tolerance and productivity in Brassica, it is necessary to comprehend the dynamic interaction between GL metabolism and enzymatic antioxidant systems. This highlights the possibility of maximizing antioxidant defences and raising the nutritional and commercial value of Brassica across the globe by utilizing genetic diversity and environmental interactions.

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Genome-Wide Identification and Expression Analysis of ESPs and NSPs Involved in Glucosinolate Hydrolysis and Insect Attack Defense in Chinese Cabbage (Brassica rapa subsp. pekinensis)

Cited by 2 publications

References 47 publications

Genome-Wide Identification of the NRT1 Family Members and Their Expression under Low-Nitrate Conditions in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Genome-Wide Identification of the NRT1 Family Members and Their Expression under Low-Nitrate Conditions in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Glucosinolates Mediated Regulation of Enzymatic Activity in Response to Oxidative Stress in Brassica spp.

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