Genome-wide identification and expression analysis of calmodulin-binding transcription activator genes in banana under drought stress

Meer, L.J. van de; Mumtaz, Sadaf; Labbo, Abdullahi Muhammad; Khan, Muhammad Jadoon; Sadiq, Irfan

doi:10.1016/j.scienta.2018.09.022

Cited by 26 publications

(23 citation statements)

References 36 publications

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“…The major basic domains, CG-1, ANK, IQ and CaMB are common in all CAMTAs and a close look into the motif sequence of CaMB domain shows residues which are highly conserved across the species. Phylogeny of CAMTAs of four species traced the evolutionary relationship among the homologues as well as orthologues, which is consistent with the previous results [15,17,18,19,21,23,24]. Some homologues show more similarity and might have co-evolved (Figure 1).…”

Section: Discussionsupporting

confidence: 90%

“…CAMTAs were first detected in Nicotiana tabaccum while studying calmodulin-binding proteins [11,12,13]. After their emergence, all multicellular eukaryotes studied to date have been reported to be equipped with variable number of CAMTA genes such as Arabidopsis thaliana (6) [13], Lycopersicum esculantum (7) [14], Medicago truncatula (7) [15], Citrus (9) [16], Populus trichocarpa (7) [17], Nicotiana tabacum (13) [18], Musa acuminata (5) [19] and Phaseolus vulgaris (8) [20]. Glycine max possesses 15 CAMTA genes and all of them differentially express under various stress conditions [21].…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of GmCAMTA12 Enhanced Drought Tolerance in Arabidopsis and Soybean

Noman

Jameel

Qiang

et al. 2019

IJMS

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Fifteen transcription factors in the CAMTA (calmodulin binding transcription activator) family of soybean were reported to differentially regulate in multiple stresses; however, their functional analyses had not yet been attempted. To characterize their role in stresses, we first comprehensively analyzed the GmCAMTA family in silico and thereafter determined their expression pattern under drought. The bioinformatics analysis revealed multiple stress-related cis-regulatory elements including ABRE, SARE, G-box and W-box, 10 unique miRNA (microRNA) targets in GmCAMTA transcripts and 48 proteins in GmCAMTAs’ interaction network. We then cloned the 2769 bp CDS (coding sequence) of GmCAMTA12 in an expression vector and overexpressed in soybean and Arabidopsis through Agrobacterium-mediated transformation. The T3 (Transgenic generation 3) stably transformed homozygous lines of Arabidopsis exhibited enhanced tolerance to drought in soil as well as on MS (Murashige and Skoog) media containing mannitol. In their drought assay, the average survival rate of transgenic Arabidopsis lines OE5 and OE12 (Overexpression Line 5 and Line 12) was 83.66% and 87.87%, respectively, which was ~30% higher than that of wild type. In addition, the germination and root length assays as well as physiological indexes such as proline and malondialdehyde contents, catalase activity and leakage of electrolytes affirmed the better performance of OE lines. Similarly, GmCAMTA12 overexpression in soybean promoted drought-efficient hairy roots in OE chimeric plants as compare to that of VC (Vector control). In parallel, the improved growth performance of OE in Hoagland-PEG (polyethylene glycol) and on MS-mannitol was revealed by their phenotypic, physiological and molecular measures. Furthermore, with the overexpression of GmCAMTA12, the downstream genes including AtAnnexin5, AtCaMHSP, At2G433110 and AtWRKY14 were upregulated in Arabidopsis. Likewise, in soybean hairy roots, GmELO, GmNAB and GmPLA1-IId were significantly upregulated as a result of GmCAMTA12 overexpression and majority of these upregulated genes in both plants possess CAMTA binding CGCG/CGTG motif in their promoters. Taken together, we report that GmCAMTA12 plays substantial role in tolerance of soybean against drought stress and could prove to be a novel candidate for engineering soybean and other plants against drought stress. Some research gaps were also identified for future studies to extend our comprehension of Ca-CaM-CAMTA-mediated stress regulatory mechanisms.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Overexpression of GmCAMTA12 Enhanced Drought Tolerance in Arabidopsis and Soybean

Noman

Jameel

Qiang

et al. 2019

IJMS

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“…The tobacco early ethylene-responsive gene (NtER1) was the very first identified CAMTA gene in tobacco which is known to be involved in senescence and death of plants. In addition, many eukaryotes have been identified to be equipped with CAMTA transporters including Arabidopsis thaliana [ 9 ], oryza sativa [ 10 ], Vitis vinifera [ 11 ], Brassica napus [ 12 ], Lycopersicum esculantum [ 13 ], Medicago truncatula [ 14 ], Citrus sinensis [ 15 ], Populus trichocarpa [ 16 ], Nicotiana tabacum [ 17 ], Musa acuminata [ 18 ], Phaseolus vulgaris [ 19 ], Zea mays [ 20 ], Solanum lycopersicum [ 21 ], Fragaria ananassa [ 22 ], and Glycine max [ 23 ]. The CAMTA-encoded proteins of plants are characterized with the presence of four functional domains known as IPT/TIG (transcription factor immunoglobulin), IQ motifs (calmodulin-binding), CG-1 (a DNA-binding domain specific to sequence), and ankyrin (ANK) repeats [ 24 – 27 ].…”

Section: Introductionmentioning

confidence: 99%

Calmodulin-binding transcription activator (CAMTA) genes family: Genome-wide survey and phylogenetic analysis in flax (Linum usitatissimum)

et al. 2020

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Flax (Linum usitatissimum) is a member of family linaceae with annual growth habit. It is included among those crops which were domesticated very early and has been used in development related studies as a model plant. In plants, Calmodulin-binding transcription activators (CAMTAs) comprise a unique set of Calmodulin-binding proteins. To elucidate the transport mechanism of secondary metabolites in flax, a genome-based study on these transporters was performed. The current investigation identified nine CAMTAs proteins, classified into three categories during phylogenetic analysis. Each group had significant evolutionary role as illustrated by the conservation of gene structures, protein domains and motif organizations over the distinctive phylogenetic classes. GO annotation suggested a link to sequence-specific DNA and protein binding, response to low temperature and transcription regulation by RNA polymerase II. The existence of different hormonal and stress responsive cis-regulatory elements in promotor region may directly correlate with the variation of their transcripts. MicroRNA target analysis revealed that various groups of miRNA families targeted the LuCAMTAs genes. Identification of CAMTA genes, miRNA studies and phylogenetic analysis may open avenues to uncover the underlying functional mechanism of this important family of genes in flax.

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“…Its activity contributes to the opening of gas spaces within parenchymatic tissues due to programmed cell death (PCD) and cell wall modifications (Nishiuchi et al 2012, Takahashi et al 2014. The formation of lysigenous aerenchyma has been studied extensively in the roots of various plant species, including barley (Settler and Waters 2003), rice (Colmer and Pedersen 2008), Zea mays (Rajhi et al 2011), Zea nicaraguensis (Mano and Omori 2013), wheat (Herzog et al 2016), and sugarcane (Grandis et al 2019. Therefore, lysigenous aerenchyma plays an important role in increasing waterlogging tolerance in dryland crops.…”

Section: Introductionmentioning

confidence: 99%

Lysigenous aerenchyma formation: responsiveness to waterlogging in oil palm roots

Nuanlaong¹,

Wuthisuthimathavee²,

Suraninpong³

2021

Biologia plant.

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ACC -1-aminocyclopropane-1-carboxylic acid; ACO -1-aminocyclopropane-1-carboxylic acid oxidase; ACO1 -1aminocyclopropane-1-carboxylate oxidase 1; ACS -1-aminocyclopropane-1-carboxylic acid synthase; ACS3 -1-aminocyclopropane -1-carboxylate synthase 3; CAMTA4 -calmodulin-binding transcription activator 4; CEL12 -endoglucanase 12; CML11 -calmodulin 11; CPI1 -cysteine proteinase inhibitor 1; ERF1 -ethylene-responsive transcription factor 1; ERF1B -ethylene-responsive transcription factor 1B; ERF91 -ethylene-responsive transcription factor 91; ERF113 -ethylene-responsive transcription factor 113; bHLH79transcription factor bHLH 79; bHLH94 -transcription factor bHLH 94; HSFA2C -heat shock protein factor A-2c; MYB1R1 -transcription factor MYB1R1; NAC29 -NAC transcription factor 29; PCD -programmed cell death; TCTP -translationally controlled tumor protein; XTH22 -xyloglucan endotransglucosylase/hydrolase protein 22; XTH23 -xyloglucan endotransglucosylase/hydro-lase protein 23; WRKY4 -transcription factor WRKY 4; TF -transcription factor.

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Genome-wide identification and expression analysis of calmodulin-binding transcription activator genes in banana under drought stress

Cited by 26 publications

References 36 publications

Overexpression of GmCAMTA12 Enhanced Drought Tolerance in Arabidopsis and Soybean

Overexpression of GmCAMTA12 Enhanced Drought Tolerance in Arabidopsis and Soybean

Calmodulin-binding transcription activator (CAMTA) genes family: Genome-wide survey and phylogenetic analysis in flax (Linum usitatissimum)

Lysigenous aerenchyma formation: responsiveness to waterlogging in oil palm roots

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