Genome-wide identification and expression of SAUR gene family in peanut (Arachis hypogaea L.) and functional identification of AhSAUR3 in drought tolerance

Abstract

Background Small auxin-upregulated RNAs (SAURs) gene family plays important roles in plant growth, development, and stress responses. However, the function of few SAUR genes is known in the peanut (Arachis hypogaea L.), one of the world’s major food legume crops. This study aimed to perform a comprehensive identification of the SAUR gene family from the peanut genome. Results The genome-wide analysis revealed that a total of 162 SAUR genes were identified in the peanut genome. The phylogenetic analysis indicated that the SAUR proteins were classified into eight subfamilies. The SAUR gene family experienced a remarkable expansion after tetraploidization, which contributed to the tandem duplication events first occurring in subgenome A and then segmental duplication events occurring between A and B subgenomes. The expression profiles based on transcriptomic data showed that SAUR genes were dominantly expressed in the leaves, pistils, perianth, and peg tips, and were widely involved in tolerance against abiotic stresses. A total of 18 AhSAUR genes selected from different subfamilies randomly presented 4 major expression patterns according to their expression characteristics in response to indole-3-acetic acid. The members from the same subfamily showed a similar expression pattern. Furthermore, the functional analysis revealed that AhSAUR3 played a negative role in response to drought tolerance. Conclusions This study provided insights into the evolution and function of the SAUR gene family and may serve as a resource for further functional research on AhSAUR genes.

Genome-Wide Analysis of the SNARE Family in Cultivated Peanut (Arachis hypogaea L.) Reveals That Some Members Are Involved in Stress Responses

“…Specifically, 22 different transcriptome samples were obtained from Genbank BioProject PRJNA291488 [ 71 ]. Transcriptome assembly and expression value estimation (Fragments per kilobase million, FPKM) were performed using StringTie (v1.3.4) with default parameters, as reported by Liu et al [ 72 ].…”

“…Specific primers were designed using Beacon Designer 7.0 software ( Table S1 ). The housekeeping gene TUA5 was used as an internal reference with primers, as described by Liu et al [ 72 ]. RT-qPCR was performed using a HiScript III RT SuperMix kit (Vazyme, Nanjing, China), as described by Lu et al [ 32 ].…”

Genome-wide identification of the SAUR gene family and screening for SmSAURs involved in root development in Salvia miltiorrhiza

“…Numerous studies have demonstrated that the expression of SAURs is induced by exogenous auxin. Liu et al (2022) report that AhSAURs from different subfamilies exhibit distinct expression characteristics in response to IAA. Notably, the expression levels of most members in subfamily II and subfamily III are up-regulated and peak after 30 minutes of IAA treatment in A. hypogaea.…”

Genome-wide identification, characterization, and expression analysis of the small auxin-up RNA gene family during zygotic and somatic embryo maturation of the cacao tree (Theobroma cacao)

“…Interestingly, the chromosomal distributions of the SAUR gene family in the genomes of other higher plant species, such as coffea [19], melon [21], and wax gourd [23] also confirmed our finding. Previously, the SAUR family is being explored in various higher plant species, such as potato [12], tomato [12], watermelon [13], cotton [14], moso bamboo [15], poplar [16], grape [17], apple [18], coffea [19], Chinese white pear [20], melon [21], loquat [22], wax gourd [23], peanut [24], pineapple [25], foxtail millet [26], cucumber [27], and longan [28]. More specifically, 31 and 38 members of the SAUR families have been recorded in coffea and moso bamboo [15,19].…”

“…To gain an insight into their functions, previous studies have been performed to analyze the SAUR families in many important crop species, including potato (Solanum tuberosum) and tomato (Solanum lycopersicum) [12], watermelon (Citrullus lanatus) [13], cotton (Gossypium spp.) [14], moso bamboo (Phyllostachys edulis) [15], poplar (Populus trichocarpa) [16], grape (Vitis vinifera) [17], apple (Malus domestica) [18], coffea (Coffea canephora) [19], Chinese white pear (Pyrus bretschneideri) [20], melon (Cucumis melo) [21], loquat (Eriobotrya japonica) [22], wax gourd (Benincasa hispida) [23], peanut (Arachis hypogaea) [24], pineapple (Ananas comosus) [25], foxtail millet (Setaria italica) [26], cucumber (Cucumis sativus) [27], and longan (Dimocarpus longan) [28]. However, this important gene family in cacao has not been recorded, even though the assembly of cacao was released [29].…”