Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato

Abstract

Heat shock transcription factors (Hsfs) play vital roles in the regulation of tolerance to various stresses in living organisms. To dissect the mechanisms of the Hsfs in potato adaptation to abiotic stresses, genome and transcriptome analyses of Hsf gene family were investigated in Solanum tuberosum L. Twenty-seven StHsf members were identified by bioinformatics and phylogenetic analyses and were classified into A, B, and C groups according to their structural and phylogenetic features. StHsfs in the same class shared similar gene structures and conserved motifs. The chromosomal location analysis showed that 27 Hsfs were located in 10 of 12 chromosomes (except chromosome 1 and chromosome 5) and that 18 of these genes formed 9 paralogous pairs. Expression profiles of StHsfs in 12 different organs and tissues uncovered distinct spatial expression patterns of these genes and their potential roles in the process of growth and development. Promoter and quantitative real-time polymerase chain reaction (qRT-PCR) detections of StHsfs were conducted and demonstrated that these genes were all responsive to various stresses. StHsf004, StHsf007, StHsf009, StHsf014, and StHsf019 were constitutively expressed under non-stress conditions, and some specific Hsfs became the predominant Hsfs in response to different abiotic stresses, indicating their important and diverse regulatory roles in adverse conditions. A co-expression network between StHsfs and StHsf -co-expressed genes was generated based on the publicly-available potato transcriptomic databases and identified key candidate StHsfs for further functional studies.

Physiological and molecular responses of two Chinese cabbage genotypes to heat stress

“…4). A Similarresult was also found in the previous studies on Chinese cabbage (Song et al 2014) as well as on other plants in which class B and class C HSFs genes were induced by heat stress (Nover et al 2001, Tang et al 2016.…”

“…Among them, class A HSFs has been relatively well studied, and the results show that HSFA1a is a master regulator and HsfA2 is a major heat stress factor in plant heat stress responses . Up to now, the HSF family has been thoroughly identified in various plants such as Arabidopsis (Nover et al 2001), tomato , soybean , potato (Tang et al 2016), maize (Lin et al 2011), as well as Chinese cabbage (Song et al 2014) with a fully sequenced genome.…”

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“…Meanwhile, there are no clear data on the time and temperature ranges of thermal exposure inducing the HSP synthesis in potato plants [25][26][27]. So, it was revealed for the first time that the heat treatment of potato plants in vitro at 39 °C for 2 h induced maximum synthesis of HSPs.…”

The Potato Genome

“…As of March, 2017, the original publication of the DM sequence has been cited more than 500 times, providing a framework for studies of gene families (Charfeddine et al 2015;Gao et al 2016;Ma et al 2016;Schreiber et al 2014;Seo et al 2016;Tang et al 2016;Van Harsselaar et al 2017), a scaffold for alignment of transcriptomic data (Campbell et al 2014;Gong et al 2015;Goyer et al 2015;Liu et al 2015;Morris et al 2014;Tang et al 2014) or a reference genome against which to discover genomic variation (Hardigan et al 2016), to cite just a few. As sequencing platforms improve, the DM assembly will likely be supplanted by that of a more robust commercial potato line.…”