Genome-wide identification and functional analysis of the TIFY gene family in response to drought in cotton

Abstract

Jasmonates control many aspects of plant biological processes. They are important for regulating plant responses to various biotic and abiotic stresses, including drought, which is one of the most serious threats to sustainable agricultural production. However, little is known regarding how jasmonate ZIM-domain (JAZ) proteins mediate jasmonic acid signals to improve stress tolerance in cotton. This represents the first comprehensive comparative study of TIFY transcription factors in both diploid A, D and tetraploid AD cotton species. In this study, we identified 21 TIFY family members in the genome of Gossypium arboretum, 28 members from Gossypium raimondii and 50 TIFY genes in Gossypium hirsutum. The phylogenetic analyses indicated the TIFY gene family could be divided into the following four subfamilies: TIFY, PPD, ZML, and JAZ subfamilies. The cotton TIFY genes have expanded through tandem duplications and segmental duplications compared with other plant species. Gene expression profile revealed temporal and tissue specificities for TIFY genes under simulated drought conditions in Gossypium arboretum. The JAZ subfamily members were the most highly expressed genes, suggesting that they have a vital role in responses to drought stress. Over-expression of GaJAZ5 gene decreased water loss, stomatal openings, and the accumulation of H2O2 in Arabidopsis thaliana. Additionally, the results of drought tolerance assays suggested that this subfamily might be involved in increasing drought tolerance. Our study provides new data regarding the genome-wide analysis of TIFY gene families and their important roles in drought tolerance in cotton species. These data may form the basis of future studies regarding the relationship between drought and jasmonic acid.Electronic supplementary materialThe online version of this article (doi:10.1007/s00438-016-1248-2) contains supplementary material, which is available to authorized users.

Genome-Wide Identification of the Tify Gene Family and Their Expression Profiles in Response to Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)

“…Segmental duplications were found in the CsTIFY family, mainly in the CsZML and CsJAZ subfamilies. Our results agree with the duplication phenomenon identified within the TIFY family, supporting the notion that duplication events play an important role in the evolution and expansion of the TIFY family in plants [6][7][8]33]. CsTIFY members in the same subfamily generally possessed a similar gene structure and shared the same distribution of conserved domain patterns, indicating the strong correlation between phylogeny and intron/exon diversification and conserved domain patterns within CsTIFY.…”

Comprehensive Analysis of the TIFY Gene Family and Its Expression Profiles under Phytohormone Treatment and Abiotic Stresses in Roots of Populus trichocarpa

“…Furthermore, PtrJAZ5 was dramatically upregulated at early response time points (3 and 6 h) under MeJA treatment. GaJAZ5 was the homologous gene of PtrJAZ5 in Gossypium arboretum; previous research had shown that when GaJAZ5-overexpression lines were treated with exogenous MeJA, the lateral root and root hair were higher in number compared to untreated transgenic lines in cotton [51]. OsJAZ1 could regulate root development by interacting with OsMYC2 through JA signaling in Rice [52].…”

Circadian control of abscisic acid biosynthesis and signalling pathways revealed by genome‐wide analysis of LHY binding targets

“…ENT BINDING 2A, -B and -C, which mediate responses to drought and salinity (Liu et al, 1998), and JAZ proteins, which function as negative regulators of jasmonic acid responses and regulate responses to drought and extremes of temperatures (Chini et al, 2007;Zhao et al, 2016).…”