Genome-wide identification and functional analysis of the glutathione S-transferase (GST) family in Pomacea canaliculata

Lin, Youfu; Xiao, Qin; Hao, Qianwen; Qian, Zijin; Li, Xuexia; Li, Peng; Li, Hong; Chen, Lian

doi:10.1016/j.ijbiomac.2021.11.038

Cited by 14 publications

(7 citation statements)

References 49 publications

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“…In the present study, we found that GSTs were significantly expressed at higher levels in the gills of Neptunea cumingii under low temperature conditions in the winter, and similar results were also obtained previously for Pomacea canaliculata. Lin et al [44] found that PcGST5 and PcGST11 were significantly upregulated in the hepatopancreas of Pomacea canaliculata after cold stress. The production of ROS significantly increased in Mytilus galloprovincialis after cold stress at 4 • C (p < 0.01), where the peak value was 8.4 times that in the control group [45].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons

Zhang,

Tian

et al. 2023

Fishes

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Neptunea cumingii is an economically important marine shellfish found in the Yellow and Bohai Seas areas of China. In this study, samples of Neptunea cumingii were collected in Zhangzidao and Yantai during spring, summer, autumn, and winter to clarify the gene expression patterns and regulatory mechanisms in their gills in different seasons. Transcriptome analysis was conducted using Neptunea cumingii gill tissues, and genes with significantly different expression levels were extracted for functional verification. The most genes with differences in expression (DEGs) were found in comparisons of the winter and summer samples. Gene enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes terms showed that these DEGs were mainly involved in immune and metabolic pathways, and they had significant effects on oxidative stress, body metabolism, and protein synthesis in Neptunea cumingii. Further screening of DEGs identified thirty-four genes related to temperature regulation, comprising thirteen genes with roles in innate immunity in shellfish, twelve genes related to oxidative stress, and nine genes related to protein synthesis and energy metabolism. Eleven DEGs were randomly selected for qPCR verification, and the results were consistent with the transcriptome analysis results. In summary, the transcriptome results differed significantly between seasons in the gill tissues of Neptunea cumingii. The expression levels of immune regulatory genes could be promoted in Neptunea cumingii during the high temperature season, whereas the expression of these genes may be inhibited in the low temperature season. The results obtained in this study provide insights into the molecular defense mechanisms that might allow Neptunea cumingii to adapt to climate change.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons

Zhang,

Tian

et al. 2023

Fishes

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“…The recent release of whole genomic data of four apple snail species, i.e., P. canaliculata, P. maculata, L. nyassanus , and M. cornuarietis , has enabled an integrated identification of the crucial stress adaptation-related gene families using a comparative genomics strategy [15]. Previously, the GST family, which plays a vital role in the regulation of detoxification and redox balance of ROS, was characterized in P. canaliculata , which revealed 30 GST genes belonging to seven classes [47]. Our study represents the most comprehensive study of the HSP superfamily in apple snails.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of the HSP gene superfamily in apple snails (Gastropoda: Ampullariidae) and expression analysis under temperature stress

Gao

et al. 2022

Preprint

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Heat shock proteins (HSPs) play important roles in the response to various stresses by being involved in cellular processes as molecular chaperones. Apple snails from the family Ampullariidae are large freshwater gastropods, while several species from the genus Pomacea have been introduced out of their native ranges and became invasive. The recent release of the genomes of four ampullariids (P. canaliculata, P. maculata, Lanistes nyassanus, and Marisa cornuarietis) has opened the opportunity for a comprehensive genome-wide analysis of the HSP superfamily. We identified that the number of HSP genes from P. canaliculata (PcaHSPs) was greater than that from the other three species. A total of 42 PcaHSPs were distributed on 12 chromosomes and were classified into the families of HSP90, HSP70, HSP60, HSP40, HSP20, and HSP10. The HSP70, HSP40 and HSP20 families have multiple members in the four species, but the other HSP families have one to three genes each. Each HSP family formed a monophyletic clade on the phylogenetic trees, except for the HSP40 family. We identified tandem duplication of paralogous genes in PcaHSP70 and PcaHSP20. The RNA-seq data show that the expression profiles of PcaHSPs in different tissues have similar patterns, except that several genes, mainly from PcaHSP20, revealed tissue-specific expression levels. Based on qPCR, we further identified that there were more HSP genes with stronger induction levels in response to hot stress than cold stress. Our findings will be helpful for future studies on stress response and adaptation focusing on HSPs in apple snails.

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“…Overlapping genes were manually removed. All candidate TRP genes were filtered by using the NCBI Conserved Domain Database (CDD, , accessed on 22 July 2022) [ 35 , 36 ] and Multiple EM for the Motif Elicitation (MEME) online tool ( , accessed on 22 July 2022) [ 37 ]. The MEME was set with the following parameters: any number of repetitions, a maximum of 10 motifs, and an optimum motif width of 6–50 residues [ 38 ], and MEME online was employed to analyze the motif structure of TRP proteins.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Phylogenetic Analysis of TRP Gene Family Members in Saurian

Lin

Dayananda

et al. 2022

Animals

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The transient receptor potential plays a critical role in the sensory nervous systems of vertebrates in response to various mechanisms and stimuli, such as environmental temperature. We studied the physiological adaptive evolution of the TRP gene in the saurian family and performed a comprehensive analysis to identify the evolution of the thermo-TRPs channels. All 251 putative TRPs were divided into 6 subfamilies, except TRPN, from the 8 saurian genomes. Multiple characteristics of these genes were analyzed. The results showed that the most conserved proteins of TRP box 1 were located in motif 1, and those of TRP box 2 were located in motif 10. The TRPA and TRPV in saurian tend to be one cluster, as a sister cluster with TRPC, and the TRPM is the root of group I. The TRPM, TRPV, and TRPP were clustered into two clades, and TRPP were organized into TRP PKD1-like and PKD2-like. Segmental duplications mainly occurred in the TRPM subfamily, and tandem duplications only occurred in the TRPV subfamily. There were 15 sites to be under positive selection for TRPA1 and TRPV2 genes. In summary, gene structure, chromosomal location, gene duplication, synteny analysis, and selective pressure at the molecular level provided some new evidence for genetic adaptation to the environment. This result provides a basis for identifying and classifying TRP genes and contributes to further elucidating their potential function in thermal sensors.

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Genome-wide identification and functional analysis of the glutathione S-transferase (GST) family in Pomacea canaliculata

Cited by 14 publications

References 49 publications

Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons

Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons

Genome-wide identification and characterization of the HSP gene superfamily in apple snails (Gastropoda: Ampullariidae) and expression analysis under temperature stress

Genome-Wide Identification and Phylogenetic Analysis of TRP Gene Family Members in Saurian

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