Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis

Czechowski, Tomasz; Stitt, Mark; Altmann, Thomas; Udvardi, Michael K.; Scheible, Wolf‐Rüdiger

doi:10.1104/pp.105.063743

Cited by 2,845 publications

(2,832 citation statements)

References 31 publications

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“…The dose-dependent effect of AsES (Fig. 3) is in agreement with observations made with other elicitors acting against B. cinerea on A. thaliana plants [31,35]. The length of the pretreatment period is not crucial for triggering resistance, but longer pretreatments lead to improved resistance (Fig.…”

Section: Ases Has the Potential As A Biocontrol Agent Against B Cinereasupporting

confidence: 88%

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The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana

et al. 2015

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AsES (Acremonium strictum Elicitor and Subtilisin) is a novel extracellular elicitor protein produced by the avirulent isolate SS71 of the opportunist strawberry fungal pathogen A. strictum. Here we describe the activity of AsES in the plant-pathogen system Arabidopsis thaliana-Botrytis cinerea. We show that AsES renders A. thaliana plants resistant to the necrotrophic pathogen B. cinerea, both locally and systemically and the defense response observed is dose-dependent. Systemic, but not local resistance is dependent on the length of exposure to AsES. The germination of the spores in vitro was not inhibited by AsES, implying that protection to B. cinerea is due to the induction of the plant defenses. These results were further supported by the findings that AsES differentially affects mutants impaired in the response to salicylic acid, jasmonic acid and ethylene, suggesting that AsES triggers the defense response through these three signaling pathways.

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Section: Ases Has the Potential As A Biocontrol Agent Against B Cinereasupporting

confidence: 88%

“…qRT-PCR was performed using Sensimix SYBR Green Kit (Bioline, www.bioline.com). Gene expression values were normalized using the gene At4g26410 previously described as a stable reference gene [31].…”

Section: Rna Extraction and Real Time Rt-pcrmentioning

confidence: 99%

The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana

et al. 2015

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“…Efficiency corrected transcript values of three biological replicates of all samples were used for determining the relative expression values. The level of each value was normalized to that of PP2A subunit gene (PDF2; At1g13320) [21]. The primer sets used in this study were previously described by Mabuchi et al [13].…”

Section: Methodsmentioning

confidence: 99%

MYB30 regulates root cell elongation under abscisic acid signaling

Sakaoka

Mabuchi

Morikami

et al. 2018

Communicative & Integrative Biology

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Reactive oxygen species (ROS) and plant hormones play important roles in regulating plant growth and stress responses as signaling molecules. Abscisic acid (ABA) is known as the key regulator of both abiotic and biotic stress responses. During stress responses, ABA is known to regulate ROS production, indicating that important crosstalk occurs between ROS and ABA signaling. We recently reported that MYB30, an MYB-type transcription factor, regulates root cell elongation under ROS signaling. In this study, we analyzed the molecular interaction between ROS and ABA signal during for root development, which is mediated through MYB30 transcriptional regulation. We showed that MYB30-regulated root cell elongation was mediated by ROS production under ABA signaling. Our findings will provide one piece of evidence of the complex cross talk between ROS and hormone signaling that regulates root development.

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“…The relative expression levels were calculated using the 2 −△△CT method. The AT4G33380 was used as an internal control (Czechowski et al 2005). All primers used are listed in Table S6.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

et al. 2018

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Small RNAs (sRNAs, including small interfering RNAs [siRNAs] and micro RNAs [miRNAs]) are key mediators of RNA silencing (or RNA interference), which play important roles in plant development and response to biotic and abiotic stimulation. Verticillium wilt is a plant vascular disease caused by the soil-borne fungal pathogens, such as Verticillium dahliae. We previously reported that V. dahliae infection increased two plant endogenous miRNAs that were exported to fungal cell to silence virulence genes. To investigate plant sRNAs in genome-wide response to V. dahliae infection, in this study, we constructed two sRNA libraries from Arabidopsis roots with and without V. dahliae infection, respectively. In total, 31 conserved miRNAs were found to be differentially expressed during the early stage of infection with V. dahliae using sRNA sequencing. Among these, the expression levels of miR160, miR164, miR166, miR167, miR390 and miR156h were confirmed by northern blot. Reverse transcription quantitative real time polymerase chain reaction results showed that the induction of miRNAs (miR160, miR164, miR166 and miR167) upon V. dahliae infection downregulated the expression of their targeted genes (ARF10, NAC1, PHV and ARF6), respectively. In addition, we identified specific phased siRNAs generated from distinct regions of two libraries. Profiling of these miRNAs and sRNAs lay the foundation for further understanding and utilising the host-induced gene silencing strategy to control plant vascular pathogens.

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Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis

Cited by 2,845 publications

References 31 publications

The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana

The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana

MYB30 regulates root cell elongation under abscisic acid signaling

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

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