Genome-Wide Identification and Transcript Analysis Reveal Potential Roles of Oligopeptide Transporter Genes in Iron Deficiency Induced Cadmium Accumulation in Peanut

Wang, Chaohui; Wang, Xueqin; Li, Jinxiu; Guan, Junhua; Tan, Zengjing; Zhang, Zheng; Shi, Gangrong

doi:10.3389/fpls.2022.894848

Cited by 12 publications

(13 citation statements)

References 76 publications

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“…Not unexpectedly, we found that Silihong showed a higher capacity for uptake and translocation of Cd from roots to shoots than Fenghua 1 under Fe-deficient conditions ( Figure 8 ). This result is consistent with our previous studies [ 37 , 49 , 51 ]. Similarly, the expression of all AhNRAMP genes except AhNRAMP2.4 was higher in the roots of Silihong than that of Fenghua 1 in the treatments of Fe deficiency with Cd exposure ( Figure 7 ).…”

Section: Discussionsupporting

confidence: 94%

“…In the present study, seven AhNRAMP genes showed low expression in most of the 22 peanut tissues under normal conditions ( Figure 6 ). Similar results have been reported in other gene families in peanut [ 49 , 50 , 51 ]. These results support the speculation that reduced expression might be beneficial to retain duplicate genes and their functional redundancy.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, 15 NRAMP genes were identified from the peanut genome, which is the largest among reported plant species, including Arabidopsis (6) [ 11 ], rice (7) [ 42 ], soybean (13) [ 43 ], common bean (7) [ 44 ], potato ( Solanum tuberosum , 5) [ 45 ], tea plant ( Camellia sinensis , 11) [ 46 ], cacao ( Theobroma cacao , 5) [ 47 ], and Spirodela polyrhiza (3) [ 48 ]. A large number of genes in the peanut genome have been reported in the gene families of metal tolerance proteins [ 49 ], zinc/iron-regulated transporter-like proteins [ 50 ], and oligopeptide transporters [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis Reveals Roles of the NRAMP Gene Family in Iron/Cadmium Interactions in Peanut

Tan

Guan

et al. 2023

IJMS

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The natural resistance-associated macrophage protein (NRAMP) family plays crucial roles in metal uptake and transport in plants. However, little is known about their functions in peanut. To understand the roles of AhNRAMP genes in iron/cadmium interactions in peanut, genome-wide identification and bioinformatics analysis was performed. A total of 15 AhNRAMP genes were identified from the peanut genome, including seven gene pairs derived from whole-genome duplication and a segmental duplicated gene. AhNRAMP proteins were divided into two distinct subfamilies. Subfamily I contains eight acid proteins with a specific conserved motif 7, which were predicted to localize in the vacuole membrane, while subfamily II includes seven basic proteins sharing specific conserved motif 10, which were localized to the plasma membrane. Subfamily I genes contained four exons, while subfamily II had 13 exons. AhNRAMP proteins are perfectly modeled on the 5m94.1.A template, suggesting a role in metal transport. Most AhNRAMP genes are preferentially expressed in roots, stamens, or developing seeds. In roots, the expression of most AhNRAMPs is induced by iron deficiency and positively correlated with cadmium accumulation, indicating crucial roles in iron/cadmium interactions. The findings provide essential information to understand the functions of AhNRAMPs in the iron/cadmium interactions in peanuts.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis Reveals Roles of the NRAMP Gene Family in Iron/Cadmium Interactions in Peanut

Tan

Guan

et al. 2023

IJMS

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“…In Arabidopsis, 17 OPT members have been identified and phylogenetically divided into two subfamilies: OPT and YSL [46]. Many OPTs have been shown to be involved in the uptake and translocation of Fe and Cd [47]. OPT1 was also identified in the present study, suggesting that it plays an important role in metal homeostasis.…”

Section: Discussionsupporting

confidence: 56%

Transcriptome Analysis Reveals the Stress Tolerance Mechanisms of Cadmium in Zoysia japonica

Xu,

Li,

et al. 2023

Plants

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Cadmium (Cd) is a severe heavy metal pollutant globally. Zoysia japonica is an important perennial warm-season turf grass that potentially plays a role in phytoremediation in Cd-polluted soil areas; however, the molecular mechanisms underlying its Cd stress response are unknown. To further investigate the early gene response pattern in Z. japonica under Cd stress, plant leaves were harvested 0, 6, 12, and 24 h after Cd stress (400 μM CdCl2) treatment and used for a time-course RNA-sequencing analysis. Twelve cDNA libraries were constructed and sequenced, and high-quality data were obtained, whose mapped rates were all higher than 94%, and more than 601 million bp of sequence were generated. A total of 5321, 6526, and 4016 differentially expressed genes were identified 6, 12, and 24 h after Cd stress treatment, respectively. A total of 1660 genes were differentially expressed at the three time points, and their gene expression profiles over time were elucidated. Based on the analysis of these genes, the important mechanisms for the Cd stress response in Z. japonica were identified. Specific genes participating in glutathione metabolism, plant hormone signal and transduction, members of protein processing in the endoplasmic reticulum, transporter proteins, transcription factors, and carbohydrate metabolism pathways were further analyzed in detail. These genes may contribute to the improvement of Cd tolerance in Z. japonica. In addition, some candidate genes were highlighted for future studies on Cd stress resistance in Z. japonica and other plants. Our results illustrate the early gene expression response of Z. japonica leaves to Cd and provide some new understanding of the molecular mechanisms of Cd stress in Zosia and Gramineae species.

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“…ahy-miR167-3p targets AhYSL3.2, AhYSL3.4, and AhYSL3.7. all of these miRNAs downregulates their corresponding genes by mRNA cleavage (Wang et al, 2022). Our miRNAs prediction results also revealed their cleaving activity.…”

Section: Discussionsupporting

confidence: 53%

In-silico identification and characterization of O-methyltransferase gene family in peanut (Arachis hypogaea L.) reveals their putative roles in development and stress tolerance

et al. 2023

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Cultivated peanut (Arachis hypogaea) is a leading protein and oil-providing crop and food source in many countries. At the same time, it is affected by a number of biotic and abiotic stresses. O-methyltransferases (OMTs) play important roles in secondary metabolism, biotic and abiotic stress tolerance. However, the OMT genes have not been comprehensively analyzed in peanut. In this study, we performed a genome-wide investigation of A. hypogaea OMT genes (AhOMTs). Gene structure, motifs distribution, phylogenetic history, genome collinearity and duplication of AhOMTs were studied in detail. Promoter cis-elements, protein-protein interactions, and micro-RNAs targeting AhOMTs were also predicted. We also comprehensively studied their expression in different tissues and under different stresses. We identified 116 OMT genes in the genome of cultivated peanut. Phylogenetically, AhOMTs were divided into three groups. Tandem and segmental duplication events played a role in the evolution of AhOMTs, and purifying selection pressure drove the duplication process. AhOMT promoters were enriched in several key cis-elements involved in growth and development, hormones, light, and defense-related activities. Micro-RNAs from 12 different families targeted 35 AhOMTs. GO enrichment analysis indicated that AhOMTs are highly enriched in transferase and catalytic activities, cellular metabolic and biosynthesis processes. Transcriptome datasets revealed that AhOMTs possessed varying expression levels in different tissues and under hormones, water, and temperature stress. Expression profiling based on qRT-PCR results also supported the transcriptome results. This study provides the theoretical basis for further work on the biological roles of AhOMT genes for developmental and stress responses.

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Genome-Wide Identification and Transcript Analysis Reveal Potential Roles of Oligopeptide Transporter Genes in Iron Deficiency Induced Cadmium Accumulation in Peanut

Cited by 12 publications

References 76 publications

Genome-Wide Identification and Expression Analysis Reveals Roles of the NRAMP Gene Family in Iron/Cadmium Interactions in Peanut

Genome-Wide Identification and Expression Analysis Reveals Roles of the NRAMP Gene Family in Iron/Cadmium Interactions in Peanut

Transcriptome Analysis Reveals the Stress Tolerance Mechanisms of Cadmium in Zoysia japonica

In-silico identification and characterization of O-methyltransferase gene family in peanut (Arachis hypogaea L.) reveals their putative roles in development and stress tolerance

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