Genome-wide identification, characterization, and expression analysis related to autotoxicity of the GST gene family in Cucumis melo L.

Wang, Jingrong; Zhang, Zhengda; Wu, Jinghua; Han, Xiao; Wang‐Pruski, Gefu; Zhang, Zhizhong

doi:10.1016/j.plaphy.2020.06.046

Cited by 21 publications

(19 citation statements)

References 41 publications

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“…Both GST and GR are important enzymes that regulate cellular reduced glutathione/glutathione disulfide (GSH/GSSG) pool in plant defense mechanisms ( Chen et al, 2012 ). In our study, the expression levels of GSTs and GR were strongly increased at the early stage of BA treatment, which were in accordance with the results from cinnamic acid-treated melon ( Wang et al, 2020 ). Moreover, exogenous diallyl disulfide (a very important allelochemical in garlic) supply increases GSH content and GST activity that might improve the resistance of tomato roots ( Cheng et al, 2016 ).…”

Section: Discussionsupporting

confidence: 91%

“…In this study, a great number of DEGs associated with stress response were significantly regulated by BA stress. Glutathione S-transferases (GSTs) catalyze the conjugation of glutathione to a wide variety of cytotoxic substrates to reduce their toxicity ( Wang et al, 2020 ). BA stress sharply and significantly induced the expression levels of GST and glutathione reductase ( GR ) genes ( Figure 4A ).…”

Section: Resultsmentioning

confidence: 99%

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Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress

et al. 2021

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Autotoxicity is known as a critical factor in replanting problem that reduces land utilization and creates economic losses. Benzoic acid (BA) is identified as a major autotoxin in peach replant problem, and causes stunted seedling growth or even death. However, the physiological and molecular mechanisms of peach response to BA stress remain elusive. Here, we comprehensively studied the morphophysiological, transcriptional, and metabolic responses of peach plants to BA toxicity. Results showed that BA stress inhibited peach seedlings growth, decreased chlorophyll contents and fluorescence levels, as well as disturbed mineral metabolism. The contents of hydrogen peroxide, superoxide anion, and malondialdehyde, as well as the total antioxidant capacity, were significantly increased under BA stress. A total of 6,319 differentially expressed genes (DEGs) were identified after BA stress, of which the DEGs related to photosynthesis, redox, and ion metabolism were greatly changed; meanwhile, numerous stress-responsive genes (HSPs, GSTs, GR, and ABC transporters) and transcription factors (MYB, AP2/ERF, NAC, bHLH, and WRKY) were noticeably altered under BA stress. BA induced metabolic reprogramming, and 74 differentially accumulated metabolites, including amino acids and derivatives, fatty acids, organic acids, sugars, and sugar alcohols, were identified in BA-stressed roots. Furthermore, an integrated analysis of genes and metabolites indicated that most of the co-mapped KEGG pathways were enriched in amino acid and carbohydrate metabolism, which implied a disturbed carbon and nitrogen metabolism after BA stress. The findings would be insightful in elucidating the mechanisms of plant response to autotoxicity stress, and help guide crops in alleviating replant problem.

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress

et al. 2021

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“…GST are encoded by a large superfamily in plants, while the amount of family members vary with species. Previously, 53, 61, and 49 GST genes were identified in Arabidopsis [34], Citrus [35], and melon [36], respectively, while only 32 GST genes were identified from pumpkin [37]. In the present study, 130 FaGST genes were found in the cultivated strawberry genome, indicating the GST family was expanded in strawberry.…”

Section: Identification and Comprehensive Analysis Of The Entire Fagst Genesmentioning

confidence: 43%

Identification of Anthocyanins-Related Glutathione S-Transferase (GST) Genes in the Genome of Cultivated Strawberry (Fragaria × ananassa)

Lin

Zhang

et al. 2020

IJMS

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Anthocyanins are responsible for the red color of strawberry, they are a subclass of flavonoids synthesized in cytosol and transferred to vacuole to form the visible color. Previous studies in model and ornamental plants indicated members of the glutathione S-transferase (GST) gene family were involved in vacuolar accumulation of anthocyanins. In the present study, a total of 130 FaGST genes were identified in the genome of cultivated strawberry (Fragaria × ananassa), which were unevenly distributed across the 28 chromosomes from the four subgenomes. Evolutionary analysis revealed the expansion of FaGST family was under stable selection and mainly drove by WGD/segmental duplication event. Classification and phylogenetic analysis indicated that all the FaGST genes were clarified into seven subclasses, among which FaGST1, FaGST37, and FaGST97 belonging to Phi class were closely related to FvRAP, an anthocyanin-related GST of wildwood strawberry, and this clade was clustered with other known anthocyanin-related GSTs. RNAseq-based expression analysis at different developmental stages of strawberry revealed that the expression of FaGST1, FaGST37, FaGST39, FaGST73, and FaGST97 was gradually increased during the fruit ripening, consistent with the anthocyanins accumulation. These expression patterns of those five FaGST genes were also significantly correlated with those of other anthocyanin biosynthetic genes such as FaCHI, FaCHS, and FaANS, as well as anthocyanin regulatory gene FaMYB10. These results indicated FaGST1, FaGST37, FaGST39, FaGST73, and FaGST97 may function in vacuolar anthocyanin accumulation in cultivated strawberry.

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“…Firstly, specific messenger RNAs WIN1 was largely induced after mechanical wounding due to cryo-treatment [49]; The accumulation of class I chitinases belonging to the GH19 family was highly consistent with WIN1 in plant defense against cold stress [50,51], which play important roles in the early developmental stage of somatic embryos [52]. GST, a soluble cytoplasmic enzyme, also played an important role in this resistant mechanism [53,54]. TIP1-1 was responsible for water loss due to being frozen at liquid nitrogen [55].…”

Section: Effects Of Cryo-treatment and Exogenous Hormones On Gene Expression During The Induction From Crsementioning

confidence: 99%

Cryo-Treatment Enhances the Embryogenicity of Mature Somatic Embryos via the lncRNA–miRNA–mRNA Network in White Spruce

Gao

Cui

Zhao

et al. 2022

IJMS

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In conifers, somatic embryogenesis is uniquely initiated from immature embryos in a narrow time window, which is considerably hindered by the difficulty to induce embryogenic tissue (ET) from other tissues, including mature somatic embryos. In this study, the embryogenic ability of newly induced ET and DNA methylation levels was detected, and whole-transcriptome sequencing analyses were carried out. The results showed that ultra-low temperature treatment significantly enhanced ET induction from mature somatic embryos, with the induction rate from 0.4% to 15.5%, but the underlying mechanisms remain unclear. The newly induced ET showed higher capability in generating mature embryos than the original ET. DNA methylation levels fluctuated during the ET induction process. Here, WGCNA analysis revealed that OPT4, TIP1-1, Chi I, GASA5, GST, LAX3, WRKY7, MYBS3, LRR-RLK, PBL7, and WIN1 genes are involved in stress response and auxin signal transduction. Through co-expression analysis, lncRNAs MSTRG.505746.1, MSTRG.1070680.1, and MSTRG.33602.1 might bind to pre-novel_miR_339 to promote the expression of WRKY7 genes for stress response; LAX3 could be protected by lncRNAs MSTRG.1070680.1 and MSTRG.33602.1 via serving as sponges for novel_miR_495 to initiate auxin signal transduction; lncRNAs MSTRG.505746.1, MSTRG.1070680.1, and MSTRG.33602.1 might serve as sponges for novel_miR_527 to enhance the expression of Chi I for early somatic embryo development. This study provides new insight into the area of stress-enhanced early somatic embryogenesis in conifers, which is also attributable to practical applications.

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Genome-wide identification, characterization, and expression analysis related to autotoxicity of the GST gene family in Cucumis melo L.

Cited by 21 publications

References 41 publications

Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress

Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress

Identification of Anthocyanins-Related Glutathione S-Transferase (GST) Genes in the Genome of Cultivated Strawberry (Fragaria × ananassa)

Cryo-Treatment Enhances the Embryogenicity of Mature Somatic Embryos via the lncRNA–miRNA–mRNA Network in White Spruce

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