Genome-wide identification, characterization of aquaporin gene family and understanding aquaporin transport system in hot pepper (Capsicum annuum L.)

Uppuluri, Lakshmi Sahitya; Motukuri, S.R. Krishna; Kumar, Dharmender

doi:10.1016/j.scienta.2021.110206

Cited by 4 publications

(2 citation statements)

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“…A phylogenetic analysis of morning glory, tomato, potato, tobacco, cotton, and citrus XIPs revealed that XIPs in morning glory are similar to those of plants belonging to Solanaceae (tomato, potato, and tobacco), which is closely related to the Convolvulaceae, to which morning glory belongs (Figure 4). A phylogenetic tree was generated by the neighbor-joining method derived from a CLUSTALW alignment of the AQP amino acid sequences of Japanese morning glory InXIPs from this study (indicated in blue) and InXIP1;1 (HM475296) [15], alongside tobacco NtXIPs [26], tomato SlXIPs [13], pepper CaXIPs [27], citrus CsXIPs [25], and flax LuXIPs [24]. Numbers on the phylogenetic tree indicate branch length.…”

Section: Aqps Have Been Highly Conserved Throughout Evolutionmentioning

confidence: 99%

“…PIPs are classified into PIP1 and PIP2 subgroups. PIP2s are characterized by a shorter N-terminus and a longer C-terminus than PIP1s [1]; these PIP characteristics were also A phylogenetic tree was generated by the neighbor-joining method derived from a CLUSTALW alignment of the AQP amino acid sequences of Japanese morning glory InXIPs from this study (indicated in blue) and InXIP1;1 (HM475296) [15], alongside tobacco NtXIPs [26], tomato SlXIPs [13], pepper CaXIPs [27], citrus CsXIPs [25], and flax LuXIPs [24]. Numbers on the phylogenetic tree indicate branch length.…”

Section: Prediction Of Aqp Transport Substrates In Morning Glorymentioning

confidence: 99%

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Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (Ipomoea nil)

Inden

Hoshino

Otagaki

et al. 2023

Plants

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The aquaporin (AQP) family, also called water channels or major intrinsic proteins, facilitate water transport. AQPs also transport low-molecular-weight solutes, including boric acid, glycerol, urea, and ammonia. Since plants are sessile, water homeostasis is crucial. Therefore, plants have developed diverse AQP variants at higher expression levels than animals. For example, 35 and 33 AQPs have been identified in Arabidopsis and rice, respectively. In the present study, we identified AQPs in morning glory (Ipomoea nil), which has been widely used as a model plant in research on flowering and floral morphology. The importance of AQPs in the opening of morning glory flowers has been reported. In the morning glory genome, 44 AQPs were identified, and their characteristics were analyzed. A phylogenetic analysis revealed five AQP subfamilies in morning glory: plasma membrane-intrinsic proteins (PIPs), tonoplast-intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs), and X-intrinsic proteins (XIPs). Further, transport substrates of morning glory AQPs were estimated based on their homology to the known AQPs in other plant species and their corresponding amino acid motifs that possess permeability pores. It was expected that PIPs are likely to transport water, carbon dioxide, and hydrogen peroxide; TIPs are likely transport water, hydrogen peroxide, ammonia, urea, and boric acid; NIPs are likely transport water, boric acid, ammonia, glycerol, and formamide; and XIPs are likely to transport water, hydrogen peroxide, and glycerol. Overall, these results suggest that AQPs are involved in water and nutrient transport in Japanese morning glory. An in silico gene expression analysis suggested the importance of AQPs in flower opening, water or nutrient uptakes from the soil to roots, and photosynthesis in morning glory. Our findings provide fundamental information that enables further study into the importance of AQPs in morning glory, including their roles in flower opening and other physiological events.

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